In Vivo Comparative Evaluation of Biocompatibility and Biodegradation of Bovine and Porcine Collagen Membranes
Mechanical barriers prevent the invasion of the surrounding soft tissues within the bone defects. This concept is known as Guided Bone Regeneration (GBR). The knowledge about the local tissue reaction and the time of degradation of absorbable membranes favors the correct clinical indication. This st...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-12-01
|
| Series: | Membranes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0375/10/12/423 |
| _version_ | 1827699987707854848 |
|---|---|
| author | Abdu Mansur Dacache Neto Suelen Cristina Sartoretto Isabelle Martins Duarte Rodrigo Figueiredo de Brito Resende Adriana Terezinha Neves Novellino Alves Carlos Fernando de Almeida Barros Mourão Jose Calasans-Maia Pietro Montemezzi Gilson Coutinho Tristão Mônica Diuana Calasans-Maia |
| author_facet | Abdu Mansur Dacache Neto Suelen Cristina Sartoretto Isabelle Martins Duarte Rodrigo Figueiredo de Brito Resende Adriana Terezinha Neves Novellino Alves Carlos Fernando de Almeida Barros Mourão Jose Calasans-Maia Pietro Montemezzi Gilson Coutinho Tristão Mônica Diuana Calasans-Maia |
| author_sort | Abdu Mansur Dacache Neto |
| collection | DOAJ |
| description | Mechanical barriers prevent the invasion of the surrounding soft tissues within the bone defects. This concept is known as Guided Bone Regeneration (GBR). The knowledge about the local tissue reaction and the time of degradation of absorbable membranes favors the correct clinical indication. This study aimed to evaluate the biocompatibility and biodegradation of a bovine collagen membrane (Lyostypt<sup>®</sup>, São Gonçalo, Brazil) and compare it to a porcine collagen membrane (Bio-Gide<sup>®</sup>) implanted in the subcutaneous tissue of mice, following ISO 10993-6:2016. Thirty Balb-C mice were randomly divided into three experimental groups, LT (Lyostypt<sup>®</sup>), BG (Bio-Gide<sup>®</sup>), and Sham (without implantation), and subdivided according to the experimental periods (7, 21, and 63 days). The BG was considered non-irritant at seven days and slight and moderate irritant at 21 and 63 days, respectively. The LT presented a small irritant reaction at seven days, a mild reaction after 21, and a reduction in the inflammatory response at 63 days. The biodegradation of the LT occurred more rapidly compared to the BG after 63 days. This study concluded that both membranes were considered biocompatible since their tissue reactions were compatible with the physiological inflammatory process; however, the Bio-Gide<sup>®</sup> was less degraded during the experimental periods, favoring the guided bone regeneration process. |
| first_indexed | 2024-03-10T14:03:02Z |
| format | Article |
| id | doaj.art-865bf00c80f34826a0cb823b77657b42 |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-10T14:03:02Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-865bf00c80f34826a0cb823b77657b422023-11-21T00:54:37ZengMDPI AGMembranes2077-03752020-12-01101242310.3390/membranes10120423In Vivo Comparative Evaluation of Biocompatibility and Biodegradation of Bovine and Porcine Collagen MembranesAbdu Mansur Dacache Neto0Suelen Cristina Sartoretto1Isabelle Martins Duarte2Rodrigo Figueiredo de Brito Resende3Adriana Terezinha Neves Novellino Alves4Carlos Fernando de Almeida Barros Mourão5Jose Calasans-Maia6Pietro Montemezzi7Gilson Coutinho Tristão8Mônica Diuana Calasans-Maia9Graduate Program, Dentistry School, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilOral Surgery Department, Dentistry School, Universidade Veiga de Almeida, Rio de Janeiro 20271-020, RJ, BrazilPost-Graduation Program in Dentistry, Universidade Veiga de Almeida, Rio de Janeiro 20271-020, RJ, BrazilOral Surgery Department, Dentistry School, Universidade Iguaçu, Nova Iguaçu 26260-045, RJ, BrazilOral Diagnosis Department, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilPost-Graduation Program in Biotechnology, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilOrthodontics Department, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilPrivate Practice, 24128 Bergamo, ItalyPeriodontics Department, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilClinical Research Laboratory in Dentistry, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilMechanical barriers prevent the invasion of the surrounding soft tissues within the bone defects. This concept is known as Guided Bone Regeneration (GBR). The knowledge about the local tissue reaction and the time of degradation of absorbable membranes favors the correct clinical indication. This study aimed to evaluate the biocompatibility and biodegradation of a bovine collagen membrane (Lyostypt<sup>®</sup>, São Gonçalo, Brazil) and compare it to a porcine collagen membrane (Bio-Gide<sup>®</sup>) implanted in the subcutaneous tissue of mice, following ISO 10993-6:2016. Thirty Balb-C mice were randomly divided into three experimental groups, LT (Lyostypt<sup>®</sup>), BG (Bio-Gide<sup>®</sup>), and Sham (without implantation), and subdivided according to the experimental periods (7, 21, and 63 days). The BG was considered non-irritant at seven days and slight and moderate irritant at 21 and 63 days, respectively. The LT presented a small irritant reaction at seven days, a mild reaction after 21, and a reduction in the inflammatory response at 63 days. The biodegradation of the LT occurred more rapidly compared to the BG after 63 days. This study concluded that both membranes were considered biocompatible since their tissue reactions were compatible with the physiological inflammatory process; however, the Bio-Gide<sup>®</sup> was less degraded during the experimental periods, favoring the guided bone regeneration process.https://www.mdpi.com/2077-0375/10/12/423biocompatibilitybiodegradationcollagen membraneguided bone regenerationsubcutaneousmice |
| spellingShingle | Abdu Mansur Dacache Neto Suelen Cristina Sartoretto Isabelle Martins Duarte Rodrigo Figueiredo de Brito Resende Adriana Terezinha Neves Novellino Alves Carlos Fernando de Almeida Barros Mourão Jose Calasans-Maia Pietro Montemezzi Gilson Coutinho Tristão Mônica Diuana Calasans-Maia In Vivo Comparative Evaluation of Biocompatibility and Biodegradation of Bovine and Porcine Collagen Membranes Membranes biocompatibility biodegradation collagen membrane guided bone regeneration subcutaneous mice |
| title | In Vivo Comparative Evaluation of Biocompatibility and Biodegradation of Bovine and Porcine Collagen Membranes |
| title_full | In Vivo Comparative Evaluation of Biocompatibility and Biodegradation of Bovine and Porcine Collagen Membranes |
| title_fullStr | In Vivo Comparative Evaluation of Biocompatibility and Biodegradation of Bovine and Porcine Collagen Membranes |
| title_full_unstemmed | In Vivo Comparative Evaluation of Biocompatibility and Biodegradation of Bovine and Porcine Collagen Membranes |
| title_short | In Vivo Comparative Evaluation of Biocompatibility and Biodegradation of Bovine and Porcine Collagen Membranes |
| title_sort | in vivo comparative evaluation of biocompatibility and biodegradation of bovine and porcine collagen membranes |
| topic | biocompatibility biodegradation collagen membrane guided bone regeneration subcutaneous mice |
| url | https://www.mdpi.com/2077-0375/10/12/423 |
| work_keys_str_mv | AT abdumansurdacacheneto invivocomparativeevaluationofbiocompatibilityandbiodegradationofbovineandporcinecollagenmembranes AT suelencristinasartoretto invivocomparativeevaluationofbiocompatibilityandbiodegradationofbovineandporcinecollagenmembranes AT isabellemartinsduarte invivocomparativeevaluationofbiocompatibilityandbiodegradationofbovineandporcinecollagenmembranes AT rodrigofigueiredodebritoresende invivocomparativeevaluationofbiocompatibilityandbiodegradationofbovineandporcinecollagenmembranes AT adrianaterezinhanevesnovellinoalves invivocomparativeevaluationofbiocompatibilityandbiodegradationofbovineandporcinecollagenmembranes AT carlosfernandodealmeidabarrosmourao invivocomparativeevaluationofbiocompatibilityandbiodegradationofbovineandporcinecollagenmembranes AT josecalasansmaia invivocomparativeevaluationofbiocompatibilityandbiodegradationofbovineandporcinecollagenmembranes AT pietromontemezzi invivocomparativeevaluationofbiocompatibilityandbiodegradationofbovineandporcinecollagenmembranes AT gilsoncoutinhotristao invivocomparativeevaluationofbiocompatibilityandbiodegradationofbovineandporcinecollagenmembranes AT monicadiuanacalasansmaia invivocomparativeevaluationofbiocompatibilityandbiodegradationofbovineandporcinecollagenmembranes |