Reviewing the Prevalence of Human Papillomavirus (HPV) in Married Women Aged 18-60 Years with Normal Pap Smear and Referring to Gynecology Clinics in Hospitals Affiliated to Isfahan University of Medical Sciences, Iran
Background: Cervical cancer is the second leading cause of mortality due to cancer among women. Since 1970, Human papillomavirus (HPV) has been proposed as the main etiologic factor for cervical cancer. High-Risk HPV types have been the major cause of cervical cancer. Therefore, it seems necessary t...
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| Format: | Article |
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Isfahan University of Medical Sciences
2012-03-01
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| Series: | مجله دانشکده پزشکی اصفهان |
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| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/1115 |
| _version_ | 1797715922709905408 |
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| author | Tajossadat Allameh Sharareh Moghim Farinaz Farahbod |
| author_facet | Tajossadat Allameh Sharareh Moghim Farinaz Farahbod |
| author_sort | Tajossadat Allameh |
| collection | DOAJ |
| description | Background: Cervical cancer is the second leading cause of mortality due to cancer among women. Since 1970, Human papillomavirus (HPV) has been proposed as the main etiologic factor for cervical cancer. High-Risk HPV types have been the major cause of cervical cancer. Therefore, it seems necessary that the most common HPV types in each population should be determined separately so that they can be used for an effective screening program, disease management, and the vaccination of the target population. To do so, the prevalence of type 6, 11, 16 and 18 of HPV were studied in married women aged 18-60 years, with normal Pap smear, referring to gynecology clinics in hospitals affiliated to Isfahan University of Medical Sciences, Iran, during 2009-2010 using the PCR technique.
Methods: In this descriptive-analytical study, 180 married women aged 18-60 years with normal Pap smear, who referred to gynecology clinics in hospitals affiliated to Isfahan University of Medical Sciences, Iran, were selected through available sampling method. After cytological screening in Pap smear, a specimen was collected from all the normal samples using Cervex-Brush® Combi. DNA samples were extracted using phenol chloroform, and the extracted DNA samples were confirmed using PCO3 primers and PCO from Beta Globin gene. In the first stage of PCR, HPV was identified through primers GP5+ and GP6+, and the samples containing HPV underwent PCR again in order to determine their genotype using specific primers for types 6, 11, 16, and 18.
Findings: In the first stage of PCR among 180 studied specimens, HPV was found in 46 specimens (25.55%). Out of 46 positive specimens, 7 specimens (15.21%) belonged to type 16, 6 specimens belonged to type 10 (13.04%), 18 specimens belonged to types 11 or 6 (21.74%), and 23 specimens (50%) belonged to other types of this virus. Among 180 studied samples, at least one of the high-risk types was found in 13 specimens (7.22%).
Conclusion: Given to the high prevalence of this virus in the present study and other conducted studies, particularly types 16 and 18 which have been proved to be risk factors for premalignant and malignant cervical lesions, and also the good effect of Gardasil vaccine, using this vaccine can be an important step towards preventing cervical cancer in the studied women. |
| first_indexed | 2024-03-12T08:13:59Z |
| format | Article |
| id | doaj.art-86601e386aaa4d629bc5fc5447a5e7bb |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T08:13:59Z |
| publishDate | 2012-03-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-86601e386aaa4d629bc5fc5447a5e7bb2023-09-02T19:01:17ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2012-03-012916320482054950Reviewing the Prevalence of Human Papillomavirus (HPV) in Married Women Aged 18-60 Years with Normal Pap Smear and Referring to Gynecology Clinics in Hospitals Affiliated to Isfahan University of Medical Sciences, IranTajossadat Allameh0Sharareh Moghim1Farinaz Farahbod2Associate Professor, Department of Obstetrics and Gynecology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranResident, Department of Obstetrics and Gynecology, School of Medicine And Student Research Committee, Isfahan University of Medical Sciences, Isfahan, IranBackground: Cervical cancer is the second leading cause of mortality due to cancer among women. Since 1970, Human papillomavirus (HPV) has been proposed as the main etiologic factor for cervical cancer. High-Risk HPV types have been the major cause of cervical cancer. Therefore, it seems necessary that the most common HPV types in each population should be determined separately so that they can be used for an effective screening program, disease management, and the vaccination of the target population. To do so, the prevalence of type 6, 11, 16 and 18 of HPV were studied in married women aged 18-60 years, with normal Pap smear, referring to gynecology clinics in hospitals affiliated to Isfahan University of Medical Sciences, Iran, during 2009-2010 using the PCR technique. Methods: In this descriptive-analytical study, 180 married women aged 18-60 years with normal Pap smear, who referred to gynecology clinics in hospitals affiliated to Isfahan University of Medical Sciences, Iran, were selected through available sampling method. After cytological screening in Pap smear, a specimen was collected from all the normal samples using Cervex-Brush® Combi. DNA samples were extracted using phenol chloroform, and the extracted DNA samples were confirmed using PCO3 primers and PCO from Beta Globin gene. In the first stage of PCR, HPV was identified through primers GP5+ and GP6+, and the samples containing HPV underwent PCR again in order to determine their genotype using specific primers for types 6, 11, 16, and 18. Findings: In the first stage of PCR among 180 studied specimens, HPV was found in 46 specimens (25.55%). Out of 46 positive specimens, 7 specimens (15.21%) belonged to type 16, 6 specimens belonged to type 10 (13.04%), 18 specimens belonged to types 11 or 6 (21.74%), and 23 specimens (50%) belonged to other types of this virus. Among 180 studied samples, at least one of the high-risk types was found in 13 specimens (7.22%). Conclusion: Given to the high prevalence of this virus in the present study and other conducted studies, particularly types 16 and 18 which have been proved to be risk factors for premalignant and malignant cervical lesions, and also the good effect of Gardasil vaccine, using this vaccine can be an important step towards preventing cervical cancer in the studied women.http://jims.mui.ac.ir/index.php/jims/article/view/1115Normal Pap smearHuman papillomavirusPCRCervical cancer |
| spellingShingle | Tajossadat Allameh Sharareh Moghim Farinaz Farahbod Reviewing the Prevalence of Human Papillomavirus (HPV) in Married Women Aged 18-60 Years with Normal Pap Smear and Referring to Gynecology Clinics in Hospitals Affiliated to Isfahan University of Medical Sciences, Iran مجله دانشکده پزشکی اصفهان Normal Pap smear Human papillomavirus PCR Cervical cancer |
| title | Reviewing the Prevalence of Human Papillomavirus (HPV) in Married Women Aged 18-60 Years with Normal Pap Smear and Referring to Gynecology Clinics in Hospitals Affiliated to Isfahan University of Medical Sciences, Iran |
| title_full | Reviewing the Prevalence of Human Papillomavirus (HPV) in Married Women Aged 18-60 Years with Normal Pap Smear and Referring to Gynecology Clinics in Hospitals Affiliated to Isfahan University of Medical Sciences, Iran |
| title_fullStr | Reviewing the Prevalence of Human Papillomavirus (HPV) in Married Women Aged 18-60 Years with Normal Pap Smear and Referring to Gynecology Clinics in Hospitals Affiliated to Isfahan University of Medical Sciences, Iran |
| title_full_unstemmed | Reviewing the Prevalence of Human Papillomavirus (HPV) in Married Women Aged 18-60 Years with Normal Pap Smear and Referring to Gynecology Clinics in Hospitals Affiliated to Isfahan University of Medical Sciences, Iran |
| title_short | Reviewing the Prevalence of Human Papillomavirus (HPV) in Married Women Aged 18-60 Years with Normal Pap Smear and Referring to Gynecology Clinics in Hospitals Affiliated to Isfahan University of Medical Sciences, Iran |
| title_sort | reviewing the prevalence of human papillomavirus hpv in married women aged 18 60 years with normal pap smear and referring to gynecology clinics in hospitals affiliated to isfahan university of medical sciences iran |
| topic | Normal Pap smear Human papillomavirus PCR Cervical cancer |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/1115 |
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