GENOTYPING CHARACTERIZATIONS ADN ANTIBIOTIC RESISTANCE OF STREPTOCOCCUS THERMOPHILUS STRAINS ISOLATED FROM DIFFERENT YOGURT BRANDS
This paper reported antibiotic resistance and PFGE genetic typing of Streptococcus thermophilus stains isolated from different yogurt brands in Chinese market. In total, 42 strains isolated from 43 yogurt samples were identified as S. thermophilus stains by 16S rDNA sequence analysis. The invest...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
2020-06-01
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| Series: | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
| Subjects: | |
| Online Access: | http://pubs.ub.ro/?pg=revues&rev=cscc6&num=202002&vol=2&aid=5102 |
| Summary: | This paper reported antibiotic resistance and PFGE genetic
typing of Streptococcus thermophilus stains isolated from different yogurt
brands in Chinese market. In total, 42 strains isolated from 43 yogurt
samples were identified as S. thermophilus stains by 16S rDNA sequence
analysis. The investigation of antibiotic-resistance profiles revealed that
those isolates were resistant to erythromycin (69.05 %), clindamycin (73.81
%), chloramphenicol (61.90 %) and fosfomycin (19.05 %), and were
susceptible to other 13 kinds of antibiotics. The genetic typing of S.
thermophilus stains was examined by pulsed-field gel electrophoresis
(PFGE) of chromosomal DNA digested with SmaI. The enzyme restriction
profiles showed the 42 S. thermophilus isolates were divided into 14 PFGE
types, and further analysis showed the PFGE profile did not completely
match with antibiotic resistance profile. The antibiotic resistance and PFGE
pattern database generated in this study suggest that the safety evaluation of
S. thermophilus should be paid more attention and will provide basic for
information of food safe assessment of yogurt. |
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| ISSN: | 1582-540X 1582-540X |