GENOTYPING CHARACTERIZATIONS ADN ANTIBIOTIC RESISTANCE OF STREPTOCOCCUS THERMOPHILUS STRAINS ISOLATED FROM DIFFERENT YOGURT BRANDS
This paper reported antibiotic resistance and PFGE genetic typing of Streptococcus thermophilus stains isolated from different yogurt brands in Chinese market. In total, 42 strains isolated from 43 yogurt samples were identified as S. thermophilus stains by 16S rDNA sequence analysis. The invest...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
2020-06-01
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| Series: | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
| Subjects: | |
| Online Access: | http://pubs.ub.ro/?pg=revues&rev=cscc6&num=202002&vol=2&aid=5102 |
| _version_ | 1818806896350461952 |
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| author | Hua W. Zeng Fei L. Xu Yun C. Guo Xiu M. Liu |
| author_facet | Hua W. Zeng Fei L. Xu Yun C. Guo Xiu M. Liu |
| author_sort | Hua W. Zeng |
| collection | DOAJ |
| description | This paper reported antibiotic resistance and PFGE genetic
typing of Streptococcus thermophilus stains isolated from different yogurt
brands in Chinese market. In total, 42 strains isolated from 43 yogurt
samples were identified as S. thermophilus stains by 16S rDNA sequence
analysis. The investigation of antibiotic-resistance profiles revealed that
those isolates were resistant to erythromycin (69.05 %), clindamycin (73.81
%), chloramphenicol (61.90 %) and fosfomycin (19.05 %), and were
susceptible to other 13 kinds of antibiotics. The genetic typing of S.
thermophilus stains was examined by pulsed-field gel electrophoresis
(PFGE) of chromosomal DNA digested with SmaI. The enzyme restriction
profiles showed the 42 S. thermophilus isolates were divided into 14 PFGE
types, and further analysis showed the PFGE profile did not completely
match with antibiotic resistance profile. The antibiotic resistance and PFGE
pattern database generated in this study suggest that the safety evaluation of
S. thermophilus should be paid more attention and will provide basic for
information of food safe assessment of yogurt. |
| first_indexed | 2024-12-18T19:17:03Z |
| format | Article |
| id | doaj.art-8660f84737ec4a498d789b57bb4fb76d |
| institution | Directory Open Access Journal |
| issn | 1582-540X 1582-540X |
| language | English |
| last_indexed | 2024-12-18T19:17:03Z |
| publishDate | 2020-06-01 |
| publisher | Alma Mater Publishing House "Vasile Alecsandri" University of Bacau |
| record_format | Article |
| series | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
| spelling | doaj.art-8660f84737ec4a498d789b57bb4fb76d2022-12-21T20:56:07ZengAlma Mater Publishing House "Vasile Alecsandri" University of BacauScientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry1582-540X1582-540X2020-06-01212163173GENOTYPING CHARACTERIZATIONS ADN ANTIBIOTIC RESISTANCE OF STREPTOCOCCUS THERMOPHILUS STRAINS ISOLATED FROM DIFFERENT YOGURT BRANDSHua W. Zeng0Fei L. Xu1 Yun C. Guo2 Xiu M. Liu3Huaibei Normal University, College of Life Sciences, 100 Dongshan Road, Huaibei, China Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 10003, China Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 10003, China Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 10003, China This paper reported antibiotic resistance and PFGE genetic typing of Streptococcus thermophilus stains isolated from different yogurt brands in Chinese market. In total, 42 strains isolated from 43 yogurt samples were identified as S. thermophilus stains by 16S rDNA sequence analysis. The investigation of antibiotic-resistance profiles revealed that those isolates were resistant to erythromycin (69.05 %), clindamycin (73.81 %), chloramphenicol (61.90 %) and fosfomycin (19.05 %), and were susceptible to other 13 kinds of antibiotics. The genetic typing of S. thermophilus stains was examined by pulsed-field gel electrophoresis (PFGE) of chromosomal DNA digested with SmaI. The enzyme restriction profiles showed the 42 S. thermophilus isolates were divided into 14 PFGE types, and further analysis showed the PFGE profile did not completely match with antibiotic resistance profile. The antibiotic resistance and PFGE pattern database generated in this study suggest that the safety evaluation of S. thermophilus should be paid more attention and will provide basic for information of food safe assessment of yogurt.http://pubs.ub.ro/?pg=revues&rev=cscc6&num=202002&vol=2&aid=5102antibiotic resistancegenotypinglactic acid bacteriayogurt16srdna |
| spellingShingle | Hua W. Zeng Fei L. Xu Yun C. Guo Xiu M. Liu GENOTYPING CHARACTERIZATIONS ADN ANTIBIOTIC RESISTANCE OF STREPTOCOCCUS THERMOPHILUS STRAINS ISOLATED FROM DIFFERENT YOGURT BRANDS Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry antibiotic resistance genotyping lactic acid bacteria yogurt 16srdna |
| title | GENOTYPING CHARACTERIZATIONS ADN ANTIBIOTIC RESISTANCE OF STREPTOCOCCUS THERMOPHILUS STRAINS ISOLATED FROM DIFFERENT YOGURT BRANDS |
| title_full | GENOTYPING CHARACTERIZATIONS ADN ANTIBIOTIC RESISTANCE OF STREPTOCOCCUS THERMOPHILUS STRAINS ISOLATED FROM DIFFERENT YOGURT BRANDS |
| title_fullStr | GENOTYPING CHARACTERIZATIONS ADN ANTIBIOTIC RESISTANCE OF STREPTOCOCCUS THERMOPHILUS STRAINS ISOLATED FROM DIFFERENT YOGURT BRANDS |
| title_full_unstemmed | GENOTYPING CHARACTERIZATIONS ADN ANTIBIOTIC RESISTANCE OF STREPTOCOCCUS THERMOPHILUS STRAINS ISOLATED FROM DIFFERENT YOGURT BRANDS |
| title_short | GENOTYPING CHARACTERIZATIONS ADN ANTIBIOTIC RESISTANCE OF STREPTOCOCCUS THERMOPHILUS STRAINS ISOLATED FROM DIFFERENT YOGURT BRANDS |
| title_sort | genotyping characterizations adn antibiotic resistance of streptococcus thermophilus strains isolated from different yogurt brands |
| topic | antibiotic resistance genotyping lactic acid bacteria yogurt 16srdna |
| url | http://pubs.ub.ro/?pg=revues&rev=cscc6&num=202002&vol=2&aid=5102 |
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