Detection of Telomerase Activity in Breast Tissue of Breast Cancer Patients and Healthy Individuals by the TRAP Assay
Abstract Background and Objective: Breast cancer is the most common cancer in women. Telomerase enzyme is one of the major factors causing the development and proliferation of immortal cells such as cancer cell. The aim of this study was to evaluate telomerase activity in breast tissues of breast c...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Golestan University of Medical Sciences
2014-04-01
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| Series: | Medical Laboratory Journal |
| Subjects: | |
| Online Access: | http://mlj.goums.ac.ir/article-1-416-en.html |
| _version_ | 1818280036016324608 |
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| author | J Asadi N Zarghami |
| author_facet | J Asadi N Zarghami |
| author_sort | J Asadi |
| collection | DOAJ |
| description | Abstract
Background and Objective: Breast cancer is the most common cancer in women. Telomerase enzyme is one of the major factors causing the development and proliferation of immortal cells such as cancer cell. The aim of this study was to evaluate telomerase activity in breast tissues of breast cancer patients and healthy people.
Material and Methods: In this descriptive study, the samples from 32 patients with malignant tumors and from 24 with benign tumors or healthy individuals were obtained .To assess the relative activity of telomerase in the samples, TRAP assay (PCR-ELISA) was used.
Results: The frequency of telomerase activity was 93.75% in patients and 8% in healthy people.
Conclusion: The results indicate that the relative activity of telomerase in tumor tissues measured by TRAP assay could be a suitable biomarker for identifying the breast cancer tissue.
Keywords: TRAP Assay breast cancer telomerase |
| first_indexed | 2024-12-12T23:42:50Z |
| format | Article |
| id | doaj.art-8664137873944b4d88261783f1a43af0 |
| institution | Directory Open Access Journal |
| issn | 2538-4449 |
| language | English |
| last_indexed | 2024-12-12T23:42:50Z |
| publishDate | 2014-04-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | Medical Laboratory Journal |
| spelling | doaj.art-8664137873944b4d88261783f1a43af02022-12-22T00:07:03ZengGolestan University of Medical SciencesMedical Laboratory Journal2538-44492014-04-01819396Detection of Telomerase Activity in Breast Tissue of Breast Cancer Patients and Healthy Individuals by the TRAP AssayJ Asadi0N Zarghami1 Abstract Background and Objective: Breast cancer is the most common cancer in women. Telomerase enzyme is one of the major factors causing the development and proliferation of immortal cells such as cancer cell. The aim of this study was to evaluate telomerase activity in breast tissues of breast cancer patients and healthy people. Material and Methods: In this descriptive study, the samples from 32 patients with malignant tumors and from 24 with benign tumors or healthy individuals were obtained .To assess the relative activity of telomerase in the samples, TRAP assay (PCR-ELISA) was used. Results: The frequency of telomerase activity was 93.75% in patients and 8% in healthy people. Conclusion: The results indicate that the relative activity of telomerase in tumor tissues measured by TRAP assay could be a suitable biomarker for identifying the breast cancer tissue. Keywords: TRAP Assay breast cancer telomerasehttp://mlj.goums.ac.ir/article-1-416-en.htmltrap assaybreast cancertelomerase |
| spellingShingle | J Asadi N Zarghami Detection of Telomerase Activity in Breast Tissue of Breast Cancer Patients and Healthy Individuals by the TRAP Assay Medical Laboratory Journal trap assay breast cancer telomerase |
| title | Detection of Telomerase Activity in Breast Tissue of Breast Cancer Patients and Healthy Individuals by the TRAP Assay |
| title_full | Detection of Telomerase Activity in Breast Tissue of Breast Cancer Patients and Healthy Individuals by the TRAP Assay |
| title_fullStr | Detection of Telomerase Activity in Breast Tissue of Breast Cancer Patients and Healthy Individuals by the TRAP Assay |
| title_full_unstemmed | Detection of Telomerase Activity in Breast Tissue of Breast Cancer Patients and Healthy Individuals by the TRAP Assay |
| title_short | Detection of Telomerase Activity in Breast Tissue of Breast Cancer Patients and Healthy Individuals by the TRAP Assay |
| title_sort | detection of telomerase activity in breast tissue of breast cancer patients and healthy individuals by the trap assay |
| topic | trap assay breast cancer telomerase |
| url | http://mlj.goums.ac.ir/article-1-416-en.html |
| work_keys_str_mv | AT jasadi detectionoftelomeraseactivityinbreasttissueofbreastcancerpatientsandhealthyindividualsbythetrapassay AT nzarghami detectionoftelomeraseactivityinbreasttissueofbreastcancerpatientsandhealthyindividualsbythetrapassay |