A Mammalian enhancer trap resource for discovering and manipulating neuronal cell types
There is a continuing need for driver strains to enable cell-type-specific manipulation in the nervous system. Each cell type expresses a unique set of genes, and recapitulating expression of marker genes by BAC transgenesis or knock-in has generated useful transgenic mouse lines. However, since gen...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2016-03-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/13503 |
| _version_ | 1818018642057494528 |
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| author | Yasuyuki Shima Ken Sugino Chris Martin Hempel Masami Shima Praveen Taneja James B Bullis Sonam Mehta Carlos Lois Sacha B Nelson |
| author_facet | Yasuyuki Shima Ken Sugino Chris Martin Hempel Masami Shima Praveen Taneja James B Bullis Sonam Mehta Carlos Lois Sacha B Nelson |
| author_sort | Yasuyuki Shima |
| collection | DOAJ |
| description | There is a continuing need for driver strains to enable cell-type-specific manipulation in the nervous system. Each cell type expresses a unique set of genes, and recapitulating expression of marker genes by BAC transgenesis or knock-in has generated useful transgenic mouse lines. However, since genes are often expressed in many cell types, many of these lines have relatively broad expression patterns. We report an alternative transgenic approach capturing distal enhancers for more focused expression. We identified an enhancer trap probe often producing restricted reporter expression and developed efficient enhancer trap screening with the PiggyBac transposon. We established more than 200 lines and found many lines that label small subsets of neurons in brain substructures, including known and novel cell types. Images and other information about each line are available online (enhancertrap.bio.brandeis.edu). |
| first_indexed | 2024-04-14T07:42:15Z |
| format | Article |
| id | doaj.art-86642de6635c47af823cd4184764c190 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:42:15Z |
| publishDate | 2016-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-86642de6635c47af823cd4184764c1902022-12-22T02:05:27ZengeLife Sciences Publications LtdeLife2050-084X2016-03-01510.7554/eLife.13503A Mammalian enhancer trap resource for discovering and manipulating neuronal cell typesYasuyuki Shima0Ken Sugino1Chris Martin Hempel2Masami Shima3Praveen Taneja4James B Bullis5Sonam Mehta6Carlos Lois7Sacha B Nelson8https://orcid.org/0000-0002-0108-8599Department of Biology and National Center for Behavioral Genomics, Brandeis University, Waltham, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesDepartment of Biology and National Center for Behavioral Genomics, Brandeis University, Waltham, United StatesDepartment of Biology and National Center for Behavioral Genomics, Brandeis University, Waltham, United StatesDepartment of Biology and National Center for Behavioral Genomics, Brandeis University, Waltham, United StatesDepartment of Biology and National Center for Behavioral Genomics, Brandeis University, Waltham, United StatesDepartment of Biology and National Center for Behavioral Genomics, Brandeis University, Waltham, United StatesDivision of Biology and Biological Engineering, Beckman Institute, California Institute of Technology, Pasadena, United StatesDepartment of Biology and National Center for Behavioral Genomics, Brandeis University, Waltham, United StatesThere is a continuing need for driver strains to enable cell-type-specific manipulation in the nervous system. Each cell type expresses a unique set of genes, and recapitulating expression of marker genes by BAC transgenesis or knock-in has generated useful transgenic mouse lines. However, since genes are often expressed in many cell types, many of these lines have relatively broad expression patterns. We report an alternative transgenic approach capturing distal enhancers for more focused expression. We identified an enhancer trap probe often producing restricted reporter expression and developed efficient enhancer trap screening with the PiggyBac transposon. We established more than 200 lines and found many lines that label small subsets of neurons in brain substructures, including known and novel cell types. Images and other information about each line are available online (enhancertrap.bio.brandeis.edu).https://elifesciences.org/articles/13503enhancer trapcell typetransgenic animals |
| spellingShingle | Yasuyuki Shima Ken Sugino Chris Martin Hempel Masami Shima Praveen Taneja James B Bullis Sonam Mehta Carlos Lois Sacha B Nelson A Mammalian enhancer trap resource for discovering and manipulating neuronal cell types eLife enhancer trap cell type transgenic animals |
| title | A Mammalian enhancer trap resource for discovering and manipulating neuronal cell types |
| title_full | A Mammalian enhancer trap resource for discovering and manipulating neuronal cell types |
| title_fullStr | A Mammalian enhancer trap resource for discovering and manipulating neuronal cell types |
| title_full_unstemmed | A Mammalian enhancer trap resource for discovering and manipulating neuronal cell types |
| title_short | A Mammalian enhancer trap resource for discovering and manipulating neuronal cell types |
| title_sort | mammalian enhancer trap resource for discovering and manipulating neuronal cell types |
| topic | enhancer trap cell type transgenic animals |
| url | https://elifesciences.org/articles/13503 |
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