Localised delivery of quercetin by thermo-sensitive PLGA-PEG-PLGA hydrogels for the treatment of brachial plexus avulsion
Accumulating evidence indicates that oxidative stress and inflammation are implicated in brachial plexus avulsion (BPA). Quercetin has anti-inflammatory, anti-oxidant, anti-apoptotic, and neuroprotective properties. This study investigated the therapeutic efficacy of a temperature-sensitive poly(D,L...
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| 格式: | 文件 |
| 语言: | English |
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Taylor & Francis Group
2020-01-01
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| 丛编: | Artificial Cells, Nanomedicine, and Biotechnology |
| 主题: | |
| 在线阅读: | https://www.tandfonline.com/doi/10.1080/21691401.2020.1770265 |
| _version_ | 1827181397810348032 |
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| author | Chao Huang Chuan Fu Zhi-Ping Qi Wen-Lai Guo Di You Rui Li Zhe Zhu |
| author_facet | Chao Huang Chuan Fu Zhi-Ping Qi Wen-Lai Guo Di You Rui Li Zhe Zhu |
| author_sort | Chao Huang |
| collection | DOAJ |
| description | Accumulating evidence indicates that oxidative stress and inflammation are implicated in brachial plexus avulsion (BPA). Quercetin has anti-inflammatory, anti-oxidant, anti-apoptotic, and neuroprotective properties. This study investigated the therapeutic efficacy of a temperature-sensitive poly(D,L-lactide-co-glycolide)-poly(ethylene-glycol)-poly(D,L-lactide-co-glycolide) (PLGA-PEG-PLGA) hydrogel sustained-release system of quercetin in BPA. In situ injections of the hydrogel loaded with different concentrations of quercetin were conducted in a rat model of BPA. Significantly reduced reactive oxygen species and interleukin-6 levels in the injured spinal cord 24 h post-surgery, increased number of anterior horn motor and functional neurons in the spinal cord 6 weeks post-surgery, thickened biceps muscle fibres and enlarged endplate area with clear structure, reduced demyelinated peripheral nerves, and significantly increased Terzis grooming test scores were found in the groups with 50 or 100 mg/mL quercetin-loaded hydrogels compared with the control and blank hydrogel groups. In conclusion, the temperature-sensitive quercetin loaded PLGA-PEG-PLGA hydrogel sustained-release system can alleviate oxidative damage and inflammation in the spinal cord, increase neuron survival rate, and promote nerve regeneration and motor function recovery in rats with early BPA. The findings suggest that this drug-loaded hydrogel has potential applications in the clinical treatment of BPA. |
| first_indexed | 2024-03-13T01:40:53Z |
| format | Article |
| id | doaj.art-86649f9fec13448aacaf8087c55fee15 |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2025-03-21T05:46:10Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-86649f9fec13448aacaf8087c55fee152024-07-23T18:44:31ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2020-01-014811010102110.1080/21691401.2020.1770265Localised delivery of quercetin by thermo-sensitive PLGA-PEG-PLGA hydrogels for the treatment of brachial plexus avulsionChao Huang0Chuan Fu1Zhi-Ping Qi2Wen-Lai Guo3Di You4Rui Li5Zhe Zhu6Department of Hand Surgery, The Second Hospital of Jilin University, Changchun, Jilin, P. R. ChinaDepartment of Orthopedic Surgery, The Second Hospital of Jilin University, Changchun, Jilin, P. R. ChinaDepartment of Orthopedic Surgery, The Second Hospital of Jilin University, Changchun, Jilin, P. R. ChinaDepartment of Hand Surgery, The Second Hospital of Jilin University, Changchun, Jilin, P. R. ChinaDepartment of Anesthesiology, The Sino-Japanese Friendship Hospital of Jilin University, Changchun, Jilin, P. R. ChinaDepartment of Hand Surgery, The Second Hospital of Jilin University, Changchun, Jilin, P. R. ChinaDepartment of Hand Surgery, The Second Hospital of Jilin University, Changchun, Jilin, P. R. ChinaAccumulating evidence indicates that oxidative stress and inflammation are implicated in brachial plexus avulsion (BPA). Quercetin has anti-inflammatory, anti-oxidant, anti-apoptotic, and neuroprotective properties. This study investigated the therapeutic efficacy of a temperature-sensitive poly(D,L-lactide-co-glycolide)-poly(ethylene-glycol)-poly(D,L-lactide-co-glycolide) (PLGA-PEG-PLGA) hydrogel sustained-release system of quercetin in BPA. In situ injections of the hydrogel loaded with different concentrations of quercetin were conducted in a rat model of BPA. Significantly reduced reactive oxygen species and interleukin-6 levels in the injured spinal cord 24 h post-surgery, increased number of anterior horn motor and functional neurons in the spinal cord 6 weeks post-surgery, thickened biceps muscle fibres and enlarged endplate area with clear structure, reduced demyelinated peripheral nerves, and significantly increased Terzis grooming test scores were found in the groups with 50 or 100 mg/mL quercetin-loaded hydrogels compared with the control and blank hydrogel groups. In conclusion, the temperature-sensitive quercetin loaded PLGA-PEG-PLGA hydrogel sustained-release system can alleviate oxidative damage and inflammation in the spinal cord, increase neuron survival rate, and promote nerve regeneration and motor function recovery in rats with early BPA. The findings suggest that this drug-loaded hydrogel has potential applications in the clinical treatment of BPA.https://www.tandfonline.com/doi/10.1080/21691401.2020.1770265PLGA-PEG-PLGAbrachial plexus avulsionquercetinnerve regenerationoxidative damageinflammatory response |
| spellingShingle | Chao Huang Chuan Fu Zhi-Ping Qi Wen-Lai Guo Di You Rui Li Zhe Zhu Localised delivery of quercetin by thermo-sensitive PLGA-PEG-PLGA hydrogels for the treatment of brachial plexus avulsion Artificial Cells, Nanomedicine, and Biotechnology PLGA-PEG-PLGA brachial plexus avulsion quercetin nerve regeneration oxidative damage inflammatory response |
| title | Localised delivery of quercetin by thermo-sensitive PLGA-PEG-PLGA hydrogels for the treatment of brachial plexus avulsion |
| title_full | Localised delivery of quercetin by thermo-sensitive PLGA-PEG-PLGA hydrogels for the treatment of brachial plexus avulsion |
| title_fullStr | Localised delivery of quercetin by thermo-sensitive PLGA-PEG-PLGA hydrogels for the treatment of brachial plexus avulsion |
| title_full_unstemmed | Localised delivery of quercetin by thermo-sensitive PLGA-PEG-PLGA hydrogels for the treatment of brachial plexus avulsion |
| title_short | Localised delivery of quercetin by thermo-sensitive PLGA-PEG-PLGA hydrogels for the treatment of brachial plexus avulsion |
| title_sort | localised delivery of quercetin by thermo sensitive plga peg plga hydrogels for the treatment of brachial plexus avulsion |
| topic | PLGA-PEG-PLGA brachial plexus avulsion quercetin nerve regeneration oxidative damage inflammatory response |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2020.1770265 |
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