Quantum dot bead-based immunochromatographic assay for the quantitative detection of triazophos
In this study, a quantum dot (QD) bead -based immunochromatographic assay (QB-ICA) was developed for the rapid, sensitive, and quantitative detection of triazophos. QBs with about 640 times brighter luminescence than the corresponding QDs were used as signal-amplified ICA probes. Under optimum condi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2019-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2019.1649638 |
| _version_ | 1819238552466096128 |
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| author | Qin Wu Peiman Wu Hong Duan Beibei Liu Yanna Shao Pan Li Cunzheng Zhang Yonghua Xiong |
| author_facet | Qin Wu Peiman Wu Hong Duan Beibei Liu Yanna Shao Pan Li Cunzheng Zhang Yonghua Xiong |
| author_sort | Qin Wu |
| collection | DOAJ |
| description | In this study, a quantum dot (QD) bead -based immunochromatographic assay (QB-ICA) was developed for the rapid, sensitive, and quantitative detection of triazophos. QBs with about 640 times brighter luminescence than the corresponding QDs were used as signal-amplified ICA probes. Under optimum conditions, QB-ICA showed a good dynamic linear detection for triazophos at 0.49–250 ng/mL with a half-maximal inhibitory concentration of 6.97 ng/mL. The average recoveries of intra- and inter assays for triazophos-spiked rice samples ranged from 80.91% to 107.00% with the coefficients of variation below 10%, indicating that QB-ICA had an acceptable accuracy for triazophos detection. The triazophos spiked samples were also detected through gas chromatography, and no significant difference (P > 0.05) was found between the two methods. In summary, the proposed QB-ICA system provided a novel platform for the rapid, sensitive, and quantitative detection of triazophos or other pesticides in food materials. |
| first_indexed | 2024-12-23T13:38:02Z |
| format | Article |
| id | doaj.art-8665bb3a3e0641d399a4046a2e2e6b13 |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-23T13:38:02Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-8665bb3a3e0641d399a4046a2e2e6b132022-12-21T17:44:58ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432019-01-0130195596710.1080/09540105.2019.16496381649638Quantum dot bead-based immunochromatographic assay for the quantitative detection of triazophosQin Wu0Peiman Wu1Hong Duan2Beibei Liu3Yanna Shao4Pan Li5Cunzheng Zhang6Yonghua Xiong7Nanchang UniversityThe Affiliated Hospital of Xuzhou Medical UniversityNanchang UniversityInstitute of Food Safety and Nutrition, Jiangsu Academy of Agricultural SciencesNanchang UniversityInstitute of Food Safety and Nutrition, Jiangsu Academy of Agricultural SciencesInstitute of Food Safety and Nutrition, Jiangsu Academy of Agricultural SciencesNanchang UniversityIn this study, a quantum dot (QD) bead -based immunochromatographic assay (QB-ICA) was developed for the rapid, sensitive, and quantitative detection of triazophos. QBs with about 640 times brighter luminescence than the corresponding QDs were used as signal-amplified ICA probes. Under optimum conditions, QB-ICA showed a good dynamic linear detection for triazophos at 0.49–250 ng/mL with a half-maximal inhibitory concentration of 6.97 ng/mL. The average recoveries of intra- and inter assays for triazophos-spiked rice samples ranged from 80.91% to 107.00% with the coefficients of variation below 10%, indicating that QB-ICA had an acceptable accuracy for triazophos detection. The triazophos spiked samples were also detected through gas chromatography, and no significant difference (P > 0.05) was found between the two methods. In summary, the proposed QB-ICA system provided a novel platform for the rapid, sensitive, and quantitative detection of triazophos or other pesticides in food materials.http://dx.doi.org/10.1080/09540105.2019.1649638quantum dot beadsimmunochromatographic assaytriazophosquantitative detection |
| spellingShingle | Qin Wu Peiman Wu Hong Duan Beibei Liu Yanna Shao Pan Li Cunzheng Zhang Yonghua Xiong Quantum dot bead-based immunochromatographic assay for the quantitative detection of triazophos Food and Agricultural Immunology quantum dot beads immunochromatographic assay triazophos quantitative detection |
| title | Quantum dot bead-based immunochromatographic assay for the quantitative detection of triazophos |
| title_full | Quantum dot bead-based immunochromatographic assay for the quantitative detection of triazophos |
| title_fullStr | Quantum dot bead-based immunochromatographic assay for the quantitative detection of triazophos |
| title_full_unstemmed | Quantum dot bead-based immunochromatographic assay for the quantitative detection of triazophos |
| title_short | Quantum dot bead-based immunochromatographic assay for the quantitative detection of triazophos |
| title_sort | quantum dot bead based immunochromatographic assay for the quantitative detection of triazophos |
| topic | quantum dot beads immunochromatographic assay triazophos quantitative detection |
| url | http://dx.doi.org/10.1080/09540105.2019.1649638 |
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