Frequency of drug resistance and immune escape mutations in the hepatitis B virus genome detected in pregnant women in the Republic of Guinea
The aim of the work is to assess the prevalence of hepatitis B virus drug resistance mutations and immune escape mutations in pregnant women in the Republic of Guinea. Materials and methods. Blood plasma samples obtained from 480 pregnant women from different regions of the Republic of Guinea wit...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Central Research Institute for Epidemiology
2023-07-01
|
| Series: | Вопросы вирусологии |
| Subjects: | |
| Online Access: | https://virusjour.crie.ru/jour/article/viewFile/12430/779 |
| _version_ | 1797782283476795392 |
|---|---|
| author | T.A.L. Balde Yulia V. Ostankova S. Boumbaly Ekaterina V. Naidenova Elena B. Zueva Elena N. Serikova Diana E. Valutite Alexander N. Schemelev Vladimir S. Davydenko Elena V. Esaulenko Areg A. Totolian |
| author_facet | T.A.L. Balde Yulia V. Ostankova S. Boumbaly Ekaterina V. Naidenova Elena B. Zueva Elena N. Serikova Diana E. Valutite Alexander N. Schemelev Vladimir S. Davydenko Elena V. Esaulenko Areg A. Totolian |
| author_sort | T.A.L. Balde |
| collection | DOAJ |
| description | The aim of the work is to assess the prevalence of hepatitis B virus drug resistance mutations and immune escape mutations in pregnant women in the Republic of Guinea.
Materials and methods. Blood plasma samples obtained from 480 pregnant women from different regions of the Republic of Guinea with laboratory-confirmed viral hepatitis B were studied. Nucleotide sequences for genotype identification and mutation detection were obtained using nested-PCR followed by Sanger sequencing, based on overlapping pairs of primers spanning the complete genome of the virus.
Results and discussion. In the examined group, the viral genotype E was the most prevalent (92.92%) compared with subgenotypes A1 (1.67%), A3 (1.46%), D1 (0.63%), D2 (1.04%) and D3 (2.29%). Among the examined HBV-infected pregnant women, 188 (39.17%) had undetectable HBsAg. Drug resistance mutations were detected in 33 individuals, which amounted to 6.88%. The following mutations were found: S78T (27.27%), L80I (24.24%), S202I (15.15%), M204I/V (42.42%). The presence of polymorphic variants not described as drug resistant has also been shown in positions associated with the development of drug resistance to tenofovir, lamivudine, telbivudine and entecavir (L80F, S202I, M204R). When analyzing the MHR and the region of a determinant, mutations were detected in 318 (66.25%) of pregnant women. In 172 of them, which amounted to 54.09%, multiple mutations were found. The amino acid substitutions in 13 positions associated with HBsAg-negative hepatitis B and/or potentially affecting HBsAg antigenicity were identified.
Conclusion. The high prevalence of immune escape and drug resistance mutations potentially associated with false-negative result of HBsAg screening, prophylaxis failure, and virological failure of therapy that has been identified among treatment naive pregnant women imposes a serious problem. |
| first_indexed | 2024-03-13T00:08:47Z |
| format | Article |
| id | doaj.art-8667ba2aaf8c419f937071243bfff570 |
| institution | Directory Open Access Journal |
| issn | 0507-4088 2411-2097 |
| language | English |
| last_indexed | 2024-03-13T00:08:47Z |
| publishDate | 2023-07-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Вопросы вирусологии |
| spelling | doaj.art-8667ba2aaf8c419f937071243bfff5702023-07-12T19:31:14ZengCentral Research Institute for EpidemiologyВопросы вирусологии0507-40882411-20972023-07-0168322824110.36233/0507-4088-1751170Frequency of drug resistance and immune escape mutations in the hepatitis B virus genome detected in pregnant women in the Republic of GuineaT.A.L. Balde0https://orcid.org/0000-0002-3808-4380Yulia V. Ostankova1https://orcid.org/0000-0003-2270-8897S. Boumbaly2https://orcid.org/0000-0002-4506-6033Ekaterina V. Naidenova3https://orcid.org/0000-0001-6474-3696Elena B. Zueva4https://orcid.org/0000-0002-0579-110XElena N. Serikova5https://orcid.org/0000-0002-0547-3945Diana E. Valutite6https://orcid.org/0000-0002-0931-102XAlexander N. Schemelev7https://orcid.org/0000-0002-3139-3674Vladimir S. Davydenko8https://orcid.org/0000-0003-0078-9681Elena V. Esaulenko9https://orcid.org/0000-0003-3669-1993Areg A. Totolian10https://orcid.org/0000-0003-4571-8799Research Institute of Applied Biology of GuineaSaint-Petersburg Pasteur InstituteResearch Institute of Applied Biology of GuineaRussian Research Anti-Plague Institute “Microbe”Saint-Petersburg Pasteur InstituteSaint-Petersburg Pasteur InstituteSaint-Petersburg Pasteur InstituteSaint-Petersburg Pasteur InstituteSaint-Petersburg Pasteur InstituteSaint-Petersburg Pasteur InstituteSaint-Petersburg Pasteur InstituteThe aim of the work is to assess the prevalence of hepatitis B virus drug resistance mutations and immune escape mutations in pregnant women in the Republic of Guinea. Materials and methods. Blood plasma samples obtained from 480 pregnant women from different regions of the Republic of Guinea with laboratory-confirmed viral hepatitis B were studied. Nucleotide sequences for genotype identification and mutation detection were obtained using nested-PCR followed by Sanger sequencing, based on overlapping pairs of primers spanning the complete genome of the virus. Results and discussion. In the examined group, the viral genotype E was the most prevalent (92.92%) compared with subgenotypes A1 (1.67%), A3 (1.46%), D1 (0.63%), D2 (1.04%) and D3 (2.29%). Among the examined HBV-infected pregnant women, 188 (39.17%) had undetectable HBsAg. Drug resistance mutations were detected in 33 individuals, which amounted to 6.88%. The following mutations were found: S78T (27.27%), L80I (24.24%), S202I (15.15%), M204I/V (42.42%). The presence of polymorphic variants not described as drug resistant has also been shown in positions associated with the development of drug resistance to tenofovir, lamivudine, telbivudine and entecavir (L80F, S202I, M204R). When analyzing the MHR and the region of a determinant, mutations were detected in 318 (66.25%) of pregnant women. In 172 of them, which amounted to 54.09%, multiple mutations were found. The amino acid substitutions in 13 positions associated with HBsAg-negative hepatitis B and/or potentially affecting HBsAg antigenicity were identified. Conclusion. The high prevalence of immune escape and drug resistance mutations potentially associated with false-negative result of HBsAg screening, prophylaxis failure, and virological failure of therapy that has been identified among treatment naive pregnant women imposes a serious problem.https://virusjour.crie.ru/jour/article/viewFile/12430/779viral hepatitis boccult hepatitis b infectionhepatitis в virusdrug resistance mutationsescape mutationsclinically significant mutationslaboratory diagnosticspregnant womenrepublic of guinea |
| spellingShingle | T.A.L. Balde Yulia V. Ostankova S. Boumbaly Ekaterina V. Naidenova Elena B. Zueva Elena N. Serikova Diana E. Valutite Alexander N. Schemelev Vladimir S. Davydenko Elena V. Esaulenko Areg A. Totolian Frequency of drug resistance and immune escape mutations in the hepatitis B virus genome detected in pregnant women in the Republic of Guinea Вопросы вирусологии viral hepatitis b occult hepatitis b infection hepatitis в virus drug resistance mutations escape mutations clinically significant mutations laboratory diagnostics pregnant women republic of guinea |
| title | Frequency of drug resistance and immune escape mutations in the hepatitis B virus genome detected in pregnant women in the Republic of Guinea |
| title_full | Frequency of drug resistance and immune escape mutations in the hepatitis B virus genome detected in pregnant women in the Republic of Guinea |
| title_fullStr | Frequency of drug resistance and immune escape mutations in the hepatitis B virus genome detected in pregnant women in the Republic of Guinea |
| title_full_unstemmed | Frequency of drug resistance and immune escape mutations in the hepatitis B virus genome detected in pregnant women in the Republic of Guinea |
| title_short | Frequency of drug resistance and immune escape mutations in the hepatitis B virus genome detected in pregnant women in the Republic of Guinea |
| title_sort | frequency of drug resistance and immune escape mutations in the hepatitis b virus genome detected in pregnant women in the republic of guinea |
| topic | viral hepatitis b occult hepatitis b infection hepatitis в virus drug resistance mutations escape mutations clinically significant mutations laboratory diagnostics pregnant women republic of guinea |
| url | https://virusjour.crie.ru/jour/article/viewFile/12430/779 |
| work_keys_str_mv | AT talbalde frequencyofdrugresistanceandimmuneescapemutationsinthehepatitisbvirusgenomedetectedinpregnantwomenintherepublicofguinea AT yuliavostankova frequencyofdrugresistanceandimmuneescapemutationsinthehepatitisbvirusgenomedetectedinpregnantwomenintherepublicofguinea AT sboumbaly frequencyofdrugresistanceandimmuneescapemutationsinthehepatitisbvirusgenomedetectedinpregnantwomenintherepublicofguinea AT ekaterinavnaidenova frequencyofdrugresistanceandimmuneescapemutationsinthehepatitisbvirusgenomedetectedinpregnantwomenintherepublicofguinea AT elenabzueva frequencyofdrugresistanceandimmuneescapemutationsinthehepatitisbvirusgenomedetectedinpregnantwomenintherepublicofguinea AT elenanserikova frequencyofdrugresistanceandimmuneescapemutationsinthehepatitisbvirusgenomedetectedinpregnantwomenintherepublicofguinea AT dianaevalutite frequencyofdrugresistanceandimmuneescapemutationsinthehepatitisbvirusgenomedetectedinpregnantwomenintherepublicofguinea AT alexandernschemelev frequencyofdrugresistanceandimmuneescapemutationsinthehepatitisbvirusgenomedetectedinpregnantwomenintherepublicofguinea AT vladimirsdavydenko frequencyofdrugresistanceandimmuneescapemutationsinthehepatitisbvirusgenomedetectedinpregnantwomenintherepublicofguinea AT elenavesaulenko frequencyofdrugresistanceandimmuneescapemutationsinthehepatitisbvirusgenomedetectedinpregnantwomenintherepublicofguinea AT aregatotolian frequencyofdrugresistanceandimmuneescapemutationsinthehepatitisbvirusgenomedetectedinpregnantwomenintherepublicofguinea |