Evaluation of Antibacterial and Antifungal Properties of Low Molecular Weight Chitosan Extracted from <i>Hermetia illucens</i> Relative to Crab Chitosan
This study shows the research on the depolymerisation of insect and crab chitosans using novel enzymes. Enzyme preparations containing recombinant chitinase Chi 418 from <i>Trichoderma harzianum</i>, chitinase Chi 403, and chitosanase Chi 402 from <i>Myceliophthora thermophila</...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/2/577 |
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| author | Adelya Khayrova Sergey Lopatin Balzhima Shagdarova Olga Sinitsyna Arkady Sinitsyn Valery Varlamov |
| author_facet | Adelya Khayrova Sergey Lopatin Balzhima Shagdarova Olga Sinitsyna Arkady Sinitsyn Valery Varlamov |
| author_sort | Adelya Khayrova |
| collection | DOAJ |
| description | This study shows the research on the depolymerisation of insect and crab chitosans using novel enzymes. Enzyme preparations containing recombinant chitinase Chi 418 from <i>Trichoderma harzianum</i>, chitinase Chi 403, and chitosanase Chi 402 from <i>Myceliophthora thermophila</i>, all belonging to the family GH18 of glycosyl hydrolases, were used to depolymerise a biopolymer, resulting in a range of chitosans with average molecular weights (M<sub>w</sub>) of 6–21 kDa. The depolymerised chitosans obtained from crustaceans and insects were studied, and their antibacterial and antifungal properties were evaluated. The results proved the significance of the chitosan’s origin, showing the potential of <i>Hermetia illucens</i> as a new source of low molecular weight chitosan with an improved biological activity. |
| first_indexed | 2024-03-10T00:49:04Z |
| format | Article |
| id | doaj.art-86681e644ac6400590173c815e1662fd |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T00:49:04Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-86681e644ac6400590173c815e1662fd2023-11-23T14:53:59ZengMDPI AGMolecules1420-30492022-01-0127257710.3390/molecules27020577Evaluation of Antibacterial and Antifungal Properties of Low Molecular Weight Chitosan Extracted from <i>Hermetia illucens</i> Relative to Crab ChitosanAdelya Khayrova0Sergey Lopatin1Balzhima Shagdarova2Olga Sinitsyna3Arkady Sinitsyn4Valery Varlamov5Institute of Bioengineering, Research Centre of Biotechnology, Russian Academy of Sciences, 119071 Moscow, RussiaInstitute of Bioengineering, Research Centre of Biotechnology, Russian Academy of Sciences, 119071 Moscow, RussiaInstitute of Bioengineering, Research Centre of Biotechnology, Russian Academy of Sciences, 119071 Moscow, RussiaDepartment of Chemistry, Moscow State University, 119991 Moscow, RussiaDepartment of Chemistry, Moscow State University, 119991 Moscow, RussiaInstitute of Bioengineering, Research Centre of Biotechnology, Russian Academy of Sciences, 119071 Moscow, RussiaThis study shows the research on the depolymerisation of insect and crab chitosans using novel enzymes. Enzyme preparations containing recombinant chitinase Chi 418 from <i>Trichoderma harzianum</i>, chitinase Chi 403, and chitosanase Chi 402 from <i>Myceliophthora thermophila</i>, all belonging to the family GH18 of glycosyl hydrolases, were used to depolymerise a biopolymer, resulting in a range of chitosans with average molecular weights (M<sub>w</sub>) of 6–21 kDa. The depolymerised chitosans obtained from crustaceans and insects were studied, and their antibacterial and antifungal properties were evaluated. The results proved the significance of the chitosan’s origin, showing the potential of <i>Hermetia illucens</i> as a new source of low molecular weight chitosan with an improved biological activity.https://www.mdpi.com/1420-3049/27/2/577chitosaninsectdepolymerisationantibacterial activityantifungal activity<i>Hermetia illucens</i> |
| spellingShingle | Adelya Khayrova Sergey Lopatin Balzhima Shagdarova Olga Sinitsyna Arkady Sinitsyn Valery Varlamov Evaluation of Antibacterial and Antifungal Properties of Low Molecular Weight Chitosan Extracted from <i>Hermetia illucens</i> Relative to Crab Chitosan Molecules chitosan insect depolymerisation antibacterial activity antifungal activity <i>Hermetia illucens</i> |
| title | Evaluation of Antibacterial and Antifungal Properties of Low Molecular Weight Chitosan Extracted from <i>Hermetia illucens</i> Relative to Crab Chitosan |
| title_full | Evaluation of Antibacterial and Antifungal Properties of Low Molecular Weight Chitosan Extracted from <i>Hermetia illucens</i> Relative to Crab Chitosan |
| title_fullStr | Evaluation of Antibacterial and Antifungal Properties of Low Molecular Weight Chitosan Extracted from <i>Hermetia illucens</i> Relative to Crab Chitosan |
| title_full_unstemmed | Evaluation of Antibacterial and Antifungal Properties of Low Molecular Weight Chitosan Extracted from <i>Hermetia illucens</i> Relative to Crab Chitosan |
| title_short | Evaluation of Antibacterial and Antifungal Properties of Low Molecular Weight Chitosan Extracted from <i>Hermetia illucens</i> Relative to Crab Chitosan |
| title_sort | evaluation of antibacterial and antifungal properties of low molecular weight chitosan extracted from i hermetia illucens i relative to crab chitosan |
| topic | chitosan insect depolymerisation antibacterial activity antifungal activity <i>Hermetia illucens</i> |
| url | https://www.mdpi.com/1420-3049/27/2/577 |
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