Performance of chromID® CARBA-SMART medium and carbapenemase inhibition method for the detection of carbapenemases among Gram negative bacilli
Background:Early detection of carbapenemase enzymes among Gram negative bacilli (GNB) is mandatory to prevent their spread. Objective: The goal of this study was to evaluate the performance of chromID® CARBA-SMART medium and carbapenemase inhibition method (CIM) for detection of carbapenemases in GN...
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| Format: | Article |
| Language: | English |
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Zagazig University, Faculty of Medicine
2022-05-01
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| Series: | Microbes and Infectious Diseases |
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| Online Access: | https://mid.journals.ekb.eg/article_220418_ac7c6bc90222f13992a1cf5436b8ec24.pdf |
| _version_ | 1817969273931300864 |
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| author | Aliaa Ghandour Wegdan Mohamad Rania Bakry Asmaa Ahmed Nahla Elsherbiny |
| author_facet | Aliaa Ghandour Wegdan Mohamad Rania Bakry Asmaa Ahmed Nahla Elsherbiny |
| author_sort | Aliaa Ghandour |
| collection | DOAJ |
| description | Background:Early detection of carbapenemase enzymes among Gram negative bacilli (GNB) is mandatory to prevent their spread. Objective: The goal of this study was to evaluate the performance of chromID® CARBA-SMART medium and carbapenemase inhibition method (CIM) for detection of carbapenemases in GNB. Methodology: A total of 142 GNB isolates were collected and tested using Vitek-2® system for identification and antimicrobial susceptibility testing The carbapenem resistant (CR) GNB were tested for carbapenemase production by phenotypic methods; chromID® CARBA-SMART medium and CIM. Carbapenemase genes (𝑏𝑙𝑎NDM-1, blaSIM-1,𝑏𝑙𝑎VIM-2, 𝑏𝑙𝑎KPC-1, blaGIM-1, blaSPM-1 and 𝑏𝑙𝑎OXA-48) were detected by PCR. Results: By minimal inhibitory concentration (MIC), 111 (78.17 %) of the 142 isolates were shown to be carbapenem resistant. Sensitivity and specificity of CIM and ChromID® CARBA-SMART medium were (100% and 66.7% respectively) for CIM and (86.7% and 100% respectively) for the medium. Resistance to carbapenem was associated with high percentages of resistance to many antibiotic classes. Carbapenemase genes were detected in 82% (91/111) of CR GNB with 𝑏𝑙𝑎NDM-1 (58.6%) and 𝑏𝑙𝑎OXA-48 (55.9 %) having the highest prevalence, followed by 𝑏𝑙𝑎KPC-1 (36 %) then 𝑏𝑙𝑎VIM-2 (10.8%) and lastly blaSPM-1 (3.6 %) and blaSIM-1 (1.8 %). The blaGIM-1 gene was not detected in any isolate. Conclusion: Carbapenemase inhibition method was found to be a very sensitive, easy, and cheap test for carbapenemase detection but needs the addition of ChromID® CARBA-SMART medium to improve the specificity of the test. This study has a high prevalence of carbapenemases among isolates with potential of rapid spread necessitating need for phenotypic tests. |
| first_indexed | 2024-04-13T20:19:11Z |
| format | Article |
| id | doaj.art-866835ed9d5e487989274e2d4dc626d6 |
| institution | Directory Open Access Journal |
| issn | 2682-4132 2682-4140 |
| language | English |
| last_indexed | 2024-04-13T20:19:11Z |
| publishDate | 2022-05-01 |
| publisher | Zagazig University, Faculty of Medicine |
| record_format | Article |
| series | Microbes and Infectious Diseases |
| spelling | doaj.art-866835ed9d5e487989274e2d4dc626d62022-12-22T02:31:35ZengZagazig University, Faculty of MedicineMicrobes and Infectious Diseases2682-41322682-41402022-05-013236637710.21608/mid.2022.118857.1242220418Performance of chromID® CARBA-SMART medium and carbapenemase inhibition method for the detection of carbapenemases among Gram negative bacilliAliaa Ghandour0Wegdan Mohamad1Rania Bakry2Asmaa Ahmed3Nahla Elsherbiny4Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, EgyptDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut-Egypt.Department of Oncological Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt.Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, EgyptDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut-Egypt.Background:Early detection of carbapenemase enzymes among Gram negative bacilli (GNB) is mandatory to prevent their spread. Objective: The goal of this study was to evaluate the performance of chromID® CARBA-SMART medium and carbapenemase inhibition method (CIM) for detection of carbapenemases in GNB. Methodology: A total of 142 GNB isolates were collected and tested using Vitek-2® system for identification and antimicrobial susceptibility testing The carbapenem resistant (CR) GNB were tested for carbapenemase production by phenotypic methods; chromID® CARBA-SMART medium and CIM. Carbapenemase genes (𝑏𝑙𝑎NDM-1, blaSIM-1,𝑏𝑙𝑎VIM-2, 𝑏𝑙𝑎KPC-1, blaGIM-1, blaSPM-1 and 𝑏𝑙𝑎OXA-48) were detected by PCR. Results: By minimal inhibitory concentration (MIC), 111 (78.17 %) of the 142 isolates were shown to be carbapenem resistant. Sensitivity and specificity of CIM and ChromID® CARBA-SMART medium were (100% and 66.7% respectively) for CIM and (86.7% and 100% respectively) for the medium. Resistance to carbapenem was associated with high percentages of resistance to many antibiotic classes. Carbapenemase genes were detected in 82% (91/111) of CR GNB with 𝑏𝑙𝑎NDM-1 (58.6%) and 𝑏𝑙𝑎OXA-48 (55.9 %) having the highest prevalence, followed by 𝑏𝑙𝑎KPC-1 (36 %) then 𝑏𝑙𝑎VIM-2 (10.8%) and lastly blaSPM-1 (3.6 %) and blaSIM-1 (1.8 %). The blaGIM-1 gene was not detected in any isolate. Conclusion: Carbapenemase inhibition method was found to be a very sensitive, easy, and cheap test for carbapenemase detection but needs the addition of ChromID® CARBA-SMART medium to improve the specificity of the test. This study has a high prevalence of carbapenemases among isolates with potential of rapid spread necessitating need for phenotypic tests.https://mid.journals.ekb.eg/article_220418_ac7c6bc90222f13992a1cf5436b8ec24.pdfcarbapenemaseinhibitiongenes |
| spellingShingle | Aliaa Ghandour Wegdan Mohamad Rania Bakry Asmaa Ahmed Nahla Elsherbiny Performance of chromID® CARBA-SMART medium and carbapenemase inhibition method for the detection of carbapenemases among Gram negative bacilli Microbes and Infectious Diseases carbapenemase inhibition genes |
| title | Performance of chromID® CARBA-SMART medium and carbapenemase inhibition method for the detection of carbapenemases among Gram negative bacilli |
| title_full | Performance of chromID® CARBA-SMART medium and carbapenemase inhibition method for the detection of carbapenemases among Gram negative bacilli |
| title_fullStr | Performance of chromID® CARBA-SMART medium and carbapenemase inhibition method for the detection of carbapenemases among Gram negative bacilli |
| title_full_unstemmed | Performance of chromID® CARBA-SMART medium and carbapenemase inhibition method for the detection of carbapenemases among Gram negative bacilli |
| title_short | Performance of chromID® CARBA-SMART medium and carbapenemase inhibition method for the detection of carbapenemases among Gram negative bacilli |
| title_sort | performance of chromid r carba smart medium and carbapenemase inhibition method for the detection of carbapenemases among gram negative bacilli |
| topic | carbapenemase inhibition genes |
| url | https://mid.journals.ekb.eg/article_220418_ac7c6bc90222f13992a1cf5436b8ec24.pdf |
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