3D-printed high-density polyethylene scaffolds with bioactive and antibacterial layer-by-layer modification for auricle reconstruction
High-density polyethylene (HDPE) is a promising material for the development of scaffold implants for auricle reconstruction. However, preparing a personalized HDPE auricle implant with favorable bioactive and antibacterial functions to promote skin tissue ingrowth is challenging. Herein, we present...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006422001594 |
| _version_ | 1828338426198360064 |
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| author | Junfeiyang Yin Jing Zhong Jiejie Wang Yilin Wang Ting Li Ling Wang Yang Yang Zhifang Zhen Yanbing Li Hongwu Zhang Shizhen Zhong Yaobin Wu Wenhua Huang |
| author_facet | Junfeiyang Yin Jing Zhong Jiejie Wang Yilin Wang Ting Li Ling Wang Yang Yang Zhifang Zhen Yanbing Li Hongwu Zhang Shizhen Zhong Yaobin Wu Wenhua Huang |
| author_sort | Junfeiyang Yin |
| collection | DOAJ |
| description | High-density polyethylene (HDPE) is a promising material for the development of scaffold implants for auricle reconstruction. However, preparing a personalized HDPE auricle implant with favorable bioactive and antibacterial functions to promote skin tissue ingrowth is challenging. Herein, we present 3D-printed HDPE auricle scaffolds with satisfactory pore size and connectivity. The layer-by-layer (LBL) approach was applied to achieve the improved bioactive and antibacterial properties of these 3D printed scaffolds. The HDPE auricle scaffolds were fabricated using an extrusion 3D printing approach, and the individualized macrostructure and porous microstructure were both adjusted by the 3D printing parameters. The polydopamine (pDA) coating method was used to construct a multilayer ε-polylysine (EPL) and fibrin (FIB) modification on the surface of the 3D HDPE scaffold via the LBL self-assembly approach, which provides the bioactive and antibacterial properties. The results of the in vivo experiments using an animal model showed that LBL-coated HDPE auricular scaffolds were able to significantly enhance skin tissue ingrowth and ameliorate the inflammatory response caused by local stress. The results of this study suggest that the combination of the 3D printing technique and surface modification provides a promising strategy for developing personalized implants with biofunctional coatings, which show great potential as a scaffold implant for auricle reconstruction applications. |
| first_indexed | 2024-04-13T22:28:38Z |
| format | Article |
| id | doaj.art-86687d87c8d24acc965e5372015a8a46 |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-04-13T22:28:38Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-86687d87c8d24acc965e5372015a8a462022-12-22T02:27:00ZengElsevierMaterials Today Bio2590-00642022-12-01161003613D-printed high-density polyethylene scaffolds with bioactive and antibacterial layer-by-layer modification for auricle reconstructionJunfeiyang Yin0Jing Zhong1Jiejie Wang2Yilin Wang3Ting Li4Ling Wang5Yang Yang6Zhifang Zhen7Yanbing Li8Hongwu Zhang9Shizhen Zhong10Yaobin Wu11Wenhua Huang12Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, ChinaGuangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China; Dermatology Hospital, Southern Medical University, Guangzhou, 510091, ChinaGuangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, ChinaGuangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, ChinaGuangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, ChinaBiomaterials Research Center, School of Biomedical Engineering, Southern Medical University, Guangzhou, 510515, ChinaGuangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, ChinaGuangdong Medical Innovation Platform for Translation of 3D Printing Application, The Third Affiliated Hospital of Southern Medical University, Guangzhou, 510000, ChinaGuangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, ChinaGuangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, ChinaGuangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China; The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, 030699, China; Corresponding author. Southern Medical University, Guangzhou, 510515, China.Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China; Corresponding author. Southern Medical University, Guangzhou, 510515, China.Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China; Guangdong Medical Innovation Platform for Translation of 3D Printing Application, The Third Affiliated Hospital of Southern Medical University, Guangzhou, 510000, China; The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, 030699, China; Corresponding author. Southern Medical University, Guangzhou, 510515, China.High-density polyethylene (HDPE) is a promising material for the development of scaffold implants for auricle reconstruction. However, preparing a personalized HDPE auricle implant with favorable bioactive and antibacterial functions to promote skin tissue ingrowth is challenging. Herein, we present 3D-printed HDPE auricle scaffolds with satisfactory pore size and connectivity. The layer-by-layer (LBL) approach was applied to achieve the improved bioactive and antibacterial properties of these 3D printed scaffolds. The HDPE auricle scaffolds were fabricated using an extrusion 3D printing approach, and the individualized macrostructure and porous microstructure were both adjusted by the 3D printing parameters. The polydopamine (pDA) coating method was used to construct a multilayer ε-polylysine (EPL) and fibrin (FIB) modification on the surface of the 3D HDPE scaffold via the LBL self-assembly approach, which provides the bioactive and antibacterial properties. The results of the in vivo experiments using an animal model showed that LBL-coated HDPE auricular scaffolds were able to significantly enhance skin tissue ingrowth and ameliorate the inflammatory response caused by local stress. The results of this study suggest that the combination of the 3D printing technique and surface modification provides a promising strategy for developing personalized implants with biofunctional coatings, which show great potential as a scaffold implant for auricle reconstruction applications.http://www.sciencedirect.com/science/article/pii/S25900064220015943D-printed high-density polyethyleneLayer-by-layer approachBioactive coatingAntibacterial coatingAuricle reconstruction |
| spellingShingle | Junfeiyang Yin Jing Zhong Jiejie Wang Yilin Wang Ting Li Ling Wang Yang Yang Zhifang Zhen Yanbing Li Hongwu Zhang Shizhen Zhong Yaobin Wu Wenhua Huang 3D-printed high-density polyethylene scaffolds with bioactive and antibacterial layer-by-layer modification for auricle reconstruction Materials Today Bio 3D-printed high-density polyethylene Layer-by-layer approach Bioactive coating Antibacterial coating Auricle reconstruction |
| title | 3D-printed high-density polyethylene scaffolds with bioactive and antibacterial layer-by-layer modification for auricle reconstruction |
| title_full | 3D-printed high-density polyethylene scaffolds with bioactive and antibacterial layer-by-layer modification for auricle reconstruction |
| title_fullStr | 3D-printed high-density polyethylene scaffolds with bioactive and antibacterial layer-by-layer modification for auricle reconstruction |
| title_full_unstemmed | 3D-printed high-density polyethylene scaffolds with bioactive and antibacterial layer-by-layer modification for auricle reconstruction |
| title_short | 3D-printed high-density polyethylene scaffolds with bioactive and antibacterial layer-by-layer modification for auricle reconstruction |
| title_sort | 3d printed high density polyethylene scaffolds with bioactive and antibacterial layer by layer modification for auricle reconstruction |
| topic | 3D-printed high-density polyethylene Layer-by-layer approach Bioactive coating Antibacterial coating Auricle reconstruction |
| url | http://www.sciencedirect.com/science/article/pii/S2590006422001594 |
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