A new liquid phase microextraction method-based reverse micelle for analysis of dexketoprofen in human plasma by HPLC-DAD
Abstract A new liquid phase microextraction method was developed by used reverse micelle-based coacervates as microextraction agents for the separation of dexketoprofen (DKT) from human plasma before its determination by high-performance liquid chromatography with photodiode-array detection (HPLC-DA...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-12-01
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| Series: | Journal of Analytical Science and Technology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40543-020-00251-5 |
| _version_ | 1819075364951949312 |
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| author | Dönay Yuvali Erkan Yilmaz İbrahim Narin |
| author_facet | Dönay Yuvali Erkan Yilmaz İbrahim Narin |
| author_sort | Dönay Yuvali |
| collection | DOAJ |
| description | Abstract A new liquid phase microextraction method was developed by used reverse micelle-based coacervates as microextraction agents for the separation of dexketoprofen (DKT) from human plasma before its determination by high-performance liquid chromatography with photodiode-array detection (HPLC-DAD). The change in the concentration of dexketoprofen in the plasma of the male and female patients was successfully monitored by using this method. The proposed method involves the use of reverse micelles of decanoic acid (DA) are dispersed in tetrahydrofuran (THF) and aqueous system. After addition of the DA and THF to the aqueous sample phase, the formation of micelles of nano and molecular size was observed in an ultrasonic bath. The solution was centrifuged, and the DKT extracted into the DA phase was analyzed by HPLC-DAD. Some analytical parameters that important in the developed procedure were examined in detail. The limit of detection (LOD), the limit of quantification (LOQ), the intraday, and inter day relative standard deviation (RSD, %) of the developed method in the plasma sample were found to be 12.8 ng mL−1, 38.8 ng mL−1, 1.7 and 3.9%, respectively. Additional/recovery studies were performed in plasma samples with proposed method, and quantitative recoveries were obtained in the range of 97–100%. The developed microextraction method was applied to human plasma that taken from volunteer patients for the determination of DKT. Graphical abstract |
| first_indexed | 2024-12-21T18:24:15Z |
| format | Article |
| id | doaj.art-8668bd492f8744cd9c00c9c4ec099f61 |
| institution | Directory Open Access Journal |
| issn | 2093-3371 |
| language | English |
| last_indexed | 2024-12-21T18:24:15Z |
| publishDate | 2020-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Analytical Science and Technology |
| spelling | doaj.art-8668bd492f8744cd9c00c9c4ec099f612022-12-21T18:54:28ZengSpringerOpenJournal of Analytical Science and Technology2093-33712020-12-011111910.1186/s40543-020-00251-5A new liquid phase microextraction method-based reverse micelle for analysis of dexketoprofen in human plasma by HPLC-DADDönay Yuvali0Erkan Yilmaz1İbrahim Narin2Faculty of Pharmacy, Deparment of Analytical Chemistry, Erciyes UniversityFaculty of Pharmacy, Deparment of Analytical Chemistry, Erciyes UniversityFaculty of Pharmacy, Deparment of Analytical Chemistry, Erciyes UniversityAbstract A new liquid phase microextraction method was developed by used reverse micelle-based coacervates as microextraction agents for the separation of dexketoprofen (DKT) from human plasma before its determination by high-performance liquid chromatography with photodiode-array detection (HPLC-DAD). The change in the concentration of dexketoprofen in the plasma of the male and female patients was successfully monitored by using this method. The proposed method involves the use of reverse micelles of decanoic acid (DA) are dispersed in tetrahydrofuran (THF) and aqueous system. After addition of the DA and THF to the aqueous sample phase, the formation of micelles of nano and molecular size was observed in an ultrasonic bath. The solution was centrifuged, and the DKT extracted into the DA phase was analyzed by HPLC-DAD. Some analytical parameters that important in the developed procedure were examined in detail. The limit of detection (LOD), the limit of quantification (LOQ), the intraday, and inter day relative standard deviation (RSD, %) of the developed method in the plasma sample were found to be 12.8 ng mL−1, 38.8 ng mL−1, 1.7 and 3.9%, respectively. Additional/recovery studies were performed in plasma samples with proposed method, and quantitative recoveries were obtained in the range of 97–100%. The developed microextraction method was applied to human plasma that taken from volunteer patients for the determination of DKT. Graphical abstracthttps://doi.org/10.1186/s40543-020-00251-5DexketoprofenDeterminationHPLCReverse micelle-based coacervatesMicroextractionPlasma |
| spellingShingle | Dönay Yuvali Erkan Yilmaz İbrahim Narin A new liquid phase microextraction method-based reverse micelle for analysis of dexketoprofen in human plasma by HPLC-DAD Journal of Analytical Science and Technology Dexketoprofen Determination HPLC Reverse micelle-based coacervates Microextraction Plasma |
| title | A new liquid phase microextraction method-based reverse micelle for analysis of dexketoprofen in human plasma by HPLC-DAD |
| title_full | A new liquid phase microextraction method-based reverse micelle for analysis of dexketoprofen in human plasma by HPLC-DAD |
| title_fullStr | A new liquid phase microextraction method-based reverse micelle for analysis of dexketoprofen in human plasma by HPLC-DAD |
| title_full_unstemmed | A new liquid phase microextraction method-based reverse micelle for analysis of dexketoprofen in human plasma by HPLC-DAD |
| title_short | A new liquid phase microextraction method-based reverse micelle for analysis of dexketoprofen in human plasma by HPLC-DAD |
| title_sort | new liquid phase microextraction method based reverse micelle for analysis of dexketoprofen in human plasma by hplc dad |
| topic | Dexketoprofen Determination HPLC Reverse micelle-based coacervates Microextraction Plasma |
| url | https://doi.org/10.1186/s40543-020-00251-5 |
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