Traditional Chinese medicine herbal extracts of Cibotium barometz, Gentiana scabra, Dioscorea batatas, Cassia tora, and Taxillus chinensis inhibit SARS-CoV replication
Development of anti-severe acute respiratory syndrome associated coronavirus (SARS-CoV) agents is pivotal to prevent the reemergence of the life-threatening disease, SARS. In this study, more than 200 extracts from Chinese medicinal herbs were evaluated for anti-SARS-CoV activities using a cell-base...
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| Format: | Article |
| Language: | English |
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Elsevier
2011-10-01
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| Series: | Journal of Traditional and Complementary Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2225411016300554 |
| _version_ | 1818291757630095360 |
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| author | Chih-Chun Wen Lie-Fen Shyur Jia-Tsrong Jan Po-Huang Liang Chih-Jung Kuo Palanisamy Arulselvan Jin-Bin Wu Sheng-Chu Kuo Ning-Sun Yang |
| author_facet | Chih-Chun Wen Lie-Fen Shyur Jia-Tsrong Jan Po-Huang Liang Chih-Jung Kuo Palanisamy Arulselvan Jin-Bin Wu Sheng-Chu Kuo Ning-Sun Yang |
| author_sort | Chih-Chun Wen |
| collection | DOAJ |
| description | Development of anti-severe acute respiratory syndrome associated coronavirus (SARS-CoV) agents is pivotal to prevent the reemergence of the life-threatening disease, SARS. In this study, more than 200 extracts from Chinese medicinal herbs were evaluated for anti-SARS-CoV activities using a cell-based assay that measured SARS-CoV-induced cytopathogenic effect (CPE) in vitro on Vero E6 cells. Six herbal extracts, one each from Gentianae Radix (龍膽 lóng dǎn; the dried rhizome of Gentiana scabra), Dioscoreae Rhizoma (山藥 shān yào; the tuber of Dioscorea batatas), Cassiae Semen (決明子 jué míng zǐ; the dried seed of Cassia tora) and Loranthi Ramus (桑寄生 sāng jì shēng; the dried stem, with leaf of Taxillus chinensis) (designated as GSH, DBM, CTH and TCH, respectively), and two from Rhizoma Cibotii (狗脊 gǒu jǐ; the dried rhizome of Cibotium barometz) (designated as CBE and CBM), were found to be potent inhibitors of SARS-CoV at concentrations between 25 and 200 μg/ml. The concentrations of the six extracts needed to inhibit 50% of Vero E6 cell proliferation (CC50) and 50% of viral replication (EC50) were determined. The resulting selective index values (SI=CC50/EC50) of the most effective extracts CBE, GSH, DBM, CTH and TCH were>59.4,> 57.5,> 62.1,> 59.4, and>92.9, respectively. Among these extracts, CBM and DBM also showed significant inhibition of SARS-CoV 3CL protease activity with IC50 values of 39 μg/ml and 44 μg/ml, respectively. Our findings suggest that these six herbal extracts may have potential as candidates for future development of anti-SARS therapeutics. |
| first_indexed | 2024-12-13T02:49:08Z |
| format | Article |
| id | doaj.art-86699d6f32bf4ce18b5013a8a13e2564 |
| institution | Directory Open Access Journal |
| issn | 2225-4110 |
| language | English |
| last_indexed | 2024-12-13T02:49:08Z |
| publishDate | 2011-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional and Complementary Medicine |
| spelling | doaj.art-86699d6f32bf4ce18b5013a8a13e25642022-12-22T00:02:07ZengElsevierJournal of Traditional and Complementary Medicine2225-41102011-10-0111415010.1016/S2225-4110(16)30055-4Traditional Chinese medicine herbal extracts of Cibotium barometz, Gentiana scabra, Dioscorea batatas, Cassia tora, and Taxillus chinensis inhibit SARS-CoV replicationChih-Chun Wen0Lie-Fen Shyur1Jia-Tsrong Jan2Po-Huang Liang3Chih-Jung Kuo4Palanisamy Arulselvan5Jin-Bin Wu6Sheng-Chu Kuo7Ning-Sun Yang8Graduate Institute of Pharmaceutical Chemistry, China Medical University, Taichung 404, TaiwanAgricultural Biotechnology Research Center, Academia Sinica, Taipei 115, TaiwanGenomics Research Center, Academia Sinica, Taipei 115, TaiwanInstitute of Biological Chemistry, Academia Sinica, Taipei 115, TaiwanInstitute of Biological Chemistry, Academia Sinica, Taipei 115, TaiwanAgricultural Biotechnology Research Center, Academia Sinica, Taipei 115, TaiwanGraduate Institute of Pharmaceutical Chemistry, China Medical University, Taichung 404, TaiwanGraduate Institute of Pharmaceutical Chemistry, China Medical University, Taichung 404, TaiwanAgricultural Biotechnology Research Center, Academia Sinica, Taipei 115, TaiwanDevelopment of anti-severe acute respiratory syndrome associated coronavirus (SARS-CoV) agents is pivotal to prevent the reemergence of the life-threatening disease, SARS. In this study, more than 200 extracts from Chinese medicinal herbs were evaluated for anti-SARS-CoV activities using a cell-based assay that measured SARS-CoV-induced cytopathogenic effect (CPE) in vitro on Vero E6 cells. Six herbal extracts, one each from Gentianae Radix (龍膽 lóng dǎn; the dried rhizome of Gentiana scabra), Dioscoreae Rhizoma (山藥 shān yào; the tuber of Dioscorea batatas), Cassiae Semen (決明子 jué míng zǐ; the dried seed of Cassia tora) and Loranthi Ramus (桑寄生 sāng jì shēng; the dried stem, with leaf of Taxillus chinensis) (designated as GSH, DBM, CTH and TCH, respectively), and two from Rhizoma Cibotii (狗脊 gǒu jǐ; the dried rhizome of Cibotium barometz) (designated as CBE and CBM), were found to be potent inhibitors of SARS-CoV at concentrations between 25 and 200 μg/ml. The concentrations of the six extracts needed to inhibit 50% of Vero E6 cell proliferation (CC50) and 50% of viral replication (EC50) were determined. The resulting selective index values (SI=CC50/EC50) of the most effective extracts CBE, GSH, DBM, CTH and TCH were>59.4,> 57.5,> 62.1,> 59.4, and>92.9, respectively. Among these extracts, CBM and DBM also showed significant inhibition of SARS-CoV 3CL protease activity with IC50 values of 39 μg/ml and 44 μg/ml, respectively. Our findings suggest that these six herbal extracts may have potential as candidates for future development of anti-SARS therapeutics.http://www.sciencedirect.com/science/article/pii/S2225411016300554Severe acute respiratory syndrome (SARS)Traditional Chinese medicine (TCM)Cytopathogenic effect (CPE)SARS 3CL proteaseCibotium barometz |
| spellingShingle | Chih-Chun Wen Lie-Fen Shyur Jia-Tsrong Jan Po-Huang Liang Chih-Jung Kuo Palanisamy Arulselvan Jin-Bin Wu Sheng-Chu Kuo Ning-Sun Yang Traditional Chinese medicine herbal extracts of Cibotium barometz, Gentiana scabra, Dioscorea batatas, Cassia tora, and Taxillus chinensis inhibit SARS-CoV replication Journal of Traditional and Complementary Medicine Severe acute respiratory syndrome (SARS) Traditional Chinese medicine (TCM) Cytopathogenic effect (CPE) SARS 3CL protease Cibotium barometz |
| title | Traditional Chinese medicine herbal extracts of Cibotium barometz, Gentiana scabra, Dioscorea batatas, Cassia tora, and Taxillus chinensis inhibit SARS-CoV replication |
| title_full | Traditional Chinese medicine herbal extracts of Cibotium barometz, Gentiana scabra, Dioscorea batatas, Cassia tora, and Taxillus chinensis inhibit SARS-CoV replication |
| title_fullStr | Traditional Chinese medicine herbal extracts of Cibotium barometz, Gentiana scabra, Dioscorea batatas, Cassia tora, and Taxillus chinensis inhibit SARS-CoV replication |
| title_full_unstemmed | Traditional Chinese medicine herbal extracts of Cibotium barometz, Gentiana scabra, Dioscorea batatas, Cassia tora, and Taxillus chinensis inhibit SARS-CoV replication |
| title_short | Traditional Chinese medicine herbal extracts of Cibotium barometz, Gentiana scabra, Dioscorea batatas, Cassia tora, and Taxillus chinensis inhibit SARS-CoV replication |
| title_sort | traditional chinese medicine herbal extracts of cibotium barometz gentiana scabra dioscorea batatas cassia tora and taxillus chinensis inhibit sars cov replication |
| topic | Severe acute respiratory syndrome (SARS) Traditional Chinese medicine (TCM) Cytopathogenic effect (CPE) SARS 3CL protease Cibotium barometz |
| url | http://www.sciencedirect.com/science/article/pii/S2225411016300554 |
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