Identification of Plasmodium falciparum HSP70-2 as a resident of the Plasmodium export compartment
The malarial parasite remodels the host erythrocyte following invasion. Well-known examples are adhesive proteins inserted into the host erythrocyte membrane, which function as virulence factors. The modification of the host erythrocyte may be mediated by a specialized domain of the endoplasmic reti...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-06-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844020308811 |
| _version_ | 1818885885395992576 |
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| author | Gladys T. Cortés Mark F. Wiser Claudio J. Gómez-Alegría |
| author_facet | Gladys T. Cortés Mark F. Wiser Claudio J. Gómez-Alegría |
| author_sort | Gladys T. Cortés |
| collection | DOAJ |
| description | The malarial parasite remodels the host erythrocyte following invasion. Well-known examples are adhesive proteins inserted into the host erythrocyte membrane, which function as virulence factors. The modification of the host erythrocyte may be mediated by a specialized domain of the endoplasmic reticulum, or Plasmodium export compartment (PEC). Previously, monoclonal antibodies recognizing the PEC were generated and one of these monoclonal antibodies recognize a 68 kDa parasite protein. In this study, the 68 kDa protein was affinity purified and analyzed by peptide mapping using mass spectrometry. The results demonstrate that the 68 kDa protein is the P. falciparum homolog of the endoplasmic reticulum resident HSP70 called PfHSP70-2. This finding is consistent with the PEC being a domain of the endoplasmic reticulum and suggests a role for PfHSP70-2 in the export of Plasmodium proteins into the host erythrocyte. |
| first_indexed | 2024-12-19T16:12:33Z |
| format | Article |
| id | doaj.art-8669d43fbb7f42deb8c925e0bdd428b4 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-19T16:12:33Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-8669d43fbb7f42deb8c925e0bdd428b42022-12-21T20:14:43ZengElsevierHeliyon2405-84402020-06-0166e04037Identification of Plasmodium falciparum HSP70-2 as a resident of the Plasmodium export compartmentGladys T. Cortés0Mark F. Wiser1Claudio J. Gómez-Alegría2Departamento de Salud Pública, Facultad de Medicina, Laboratorio de Equipos Comunes, Universidad Nacional de Colombia, Calle 45 No. 30-03, Edificio 471, Bogotá, Colombia; Corresponding author.Department of Tropical Medicine, Tulane University School of Public Health and Tropical Medicine, New Orleans, LA, USADepartamento de Farmacia, Facultad de Ciencias, Universidad Nacional de Colombia, Grupo UNIMOL, ColombiaThe malarial parasite remodels the host erythrocyte following invasion. Well-known examples are adhesive proteins inserted into the host erythrocyte membrane, which function as virulence factors. The modification of the host erythrocyte may be mediated by a specialized domain of the endoplasmic reticulum, or Plasmodium export compartment (PEC). Previously, monoclonal antibodies recognizing the PEC were generated and one of these monoclonal antibodies recognize a 68 kDa parasite protein. In this study, the 68 kDa protein was affinity purified and analyzed by peptide mapping using mass spectrometry. The results demonstrate that the 68 kDa protein is the P. falciparum homolog of the endoplasmic reticulum resident HSP70 called PfHSP70-2. This finding is consistent with the PEC being a domain of the endoplasmic reticulum and suggests a role for PfHSP70-2 in the export of Plasmodium proteins into the host erythrocyte.http://www.sciencedirect.com/science/article/pii/S2405844020308811BiochemistryBiological sciencesCell biologyMedical microbiologyMicrobiologyMolecular biology |
| spellingShingle | Gladys T. Cortés Mark F. Wiser Claudio J. Gómez-Alegría Identification of Plasmodium falciparum HSP70-2 as a resident of the Plasmodium export compartment Heliyon Biochemistry Biological sciences Cell biology Medical microbiology Microbiology Molecular biology |
| title | Identification of Plasmodium falciparum HSP70-2 as a resident of the Plasmodium export compartment |
| title_full | Identification of Plasmodium falciparum HSP70-2 as a resident of the Plasmodium export compartment |
| title_fullStr | Identification of Plasmodium falciparum HSP70-2 as a resident of the Plasmodium export compartment |
| title_full_unstemmed | Identification of Plasmodium falciparum HSP70-2 as a resident of the Plasmodium export compartment |
| title_short | Identification of Plasmodium falciparum HSP70-2 as a resident of the Plasmodium export compartment |
| title_sort | identification of plasmodium falciparum hsp70 2 as a resident of the plasmodium export compartment |
| topic | Biochemistry Biological sciences Cell biology Medical microbiology Microbiology Molecular biology |
| url | http://www.sciencedirect.com/science/article/pii/S2405844020308811 |
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