Hydrogen Peroxide- and Nitric Oxide-mediated Disease Control of Bacterial Wilt in Tomato Plants
Reactive oxygen species (ROS) generation in tomato plants by Ralstonia solanacearum infection and the role of hydrogen peroxide (H₂O₂) and nitric oxide in tomato bacterial wilt control were demonstrated. During disease development of tomato bacterial wilt, accumulation of superoxide anion (O₂−) and...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2013-12-01
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| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174819/ |
| _version_ | 1811202569990569984 |
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| author | Jeum Kyu Hong Su Ran Kang Yeon Hwa Kim Dong June Yoon Do Hoon Kim Hyeon Ji Kim Chang Hyun Sung Han Sol Kang Chang Won Choi Seong Hwan Kim Young Shik Kim |
| author_facet | Jeum Kyu Hong Su Ran Kang Yeon Hwa Kim Dong June Yoon Do Hoon Kim Hyeon Ji Kim Chang Hyun Sung Han Sol Kang Chang Won Choi Seong Hwan Kim Young Shik Kim |
| author_sort | Jeum Kyu Hong |
| collection | DOAJ |
| description | Reactive oxygen species (ROS) generation in tomato plants by Ralstonia solanacearum infection and the role of hydrogen peroxide (H₂O₂) and nitric oxide in tomato bacterial wilt control were demonstrated. During disease development of tomato bacterial wilt, accumulation of superoxide anion (O₂−) and H₂O₂ was observed and lipid peroxidation also occurred in the tomato leaf tissues. High doses of H₂O₂and sodium nitroprusside (SNP) nitric oxide donor showed phytotoxicity to detached tomato leaves 1 day after petiole feeding showing reduced fresh weight. Both H₂O₂and SNP have in vitro antibacterial activities against R. solanacearum in a dose-dependent manner, as well as plant protection in detached tomato leaves against bacterial wilt by 10⁶ and 10⁷ cfu/ml of R. solanacearum. H₂O₂- and SNP-mediated protection was also evaluated in pots using soil-drench treatment with the bacterial inoculation, and relative ‘area under the disease progressive curve (AUDPC)’ was calculated to compare disease protection by H₂O₂ and/or SNP with untreated control. Neither H₂O₂ nor SNP protect the tomato seedlings from the bacterial wilt, but H₂O₂+ SNP mixture significantly decreased disease severity with reduced relative AUDPC. These results suggest that H₂O₂ and SNP could be used together to control bacterial wilt in tomato plants as bactericidal agents. |
| first_indexed | 2024-04-12T02:40:52Z |
| format | Article |
| id | doaj.art-8669e891ba074a3a9c7a5deb72ab1d42 |
| institution | Directory Open Access Journal |
| issn | 1598-2254 |
| language | English |
| last_indexed | 2024-04-12T02:40:52Z |
| publishDate | 2013-12-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-8669e891ba074a3a9c7a5deb72ab1d422022-12-22T03:51:18ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542013-12-0129438639610.5423/PPJ.OA.04.2013.0043PPJ.OA.04.2013.0043Hydrogen Peroxide- and Nitric Oxide-mediated Disease Control of Bacterial Wilt in Tomato PlantsJeum Kyu Hong0Su Ran Kang1Yeon Hwa Kim2Dong June Yoon3Do Hoon Kim4Hyeon Ji Kim5Chang Hyun Sung6Han Sol Kang7Chang Won Choi8Seong Hwan Kim9Young Shik Kim10Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech), Jinju 660-758, KoreaDepartment of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech), Jinju 660-758, KoreaDepartment of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech), Jinju 660-758, KoreaDepartment of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech), Jinju 660-758, KoreaDepartment of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech), Jinju 660-758, KoreaDepartment of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech), Jinju 660-758, KoreaDepartment of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech), Jinju 660-758, KoreaDepartment of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech), Jinju 660-758, KoreaDepartment of Biology and Medical Science, Paichai University, Daejeon 302-735, KoreaDepartment of Microbiology and Institute of Basic Sciences, Dankook University, Cheonan 330-714, KoreaDepartment of Plant Science and Food Biotechnology, Sangmyung University, Cheonan 330-720, KoreaReactive oxygen species (ROS) generation in tomato plants by Ralstonia solanacearum infection and the role of hydrogen peroxide (H₂O₂) and nitric oxide in tomato bacterial wilt control were demonstrated. During disease development of tomato bacterial wilt, accumulation of superoxide anion (O₂−) and H₂O₂ was observed and lipid peroxidation also occurred in the tomato leaf tissues. High doses of H₂O₂and sodium nitroprusside (SNP) nitric oxide donor showed phytotoxicity to detached tomato leaves 1 day after petiole feeding showing reduced fresh weight. Both H₂O₂and SNP have in vitro antibacterial activities against R. solanacearum in a dose-dependent manner, as well as plant protection in detached tomato leaves against bacterial wilt by 10⁶ and 10⁷ cfu/ml of R. solanacearum. H₂O₂- and SNP-mediated protection was also evaluated in pots using soil-drench treatment with the bacterial inoculation, and relative ‘area under the disease progressive curve (AUDPC)’ was calculated to compare disease protection by H₂O₂ and/or SNP with untreated control. Neither H₂O₂ nor SNP protect the tomato seedlings from the bacterial wilt, but H₂O₂+ SNP mixture significantly decreased disease severity with reduced relative AUDPC. These results suggest that H₂O₂ and SNP could be used together to control bacterial wilt in tomato plants as bactericidal agents.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174819/antibacterial agentshydrogen peroxidenitric oxideRalstonia solanacearumtomato bacterial wilt |
| spellingShingle | Jeum Kyu Hong Su Ran Kang Yeon Hwa Kim Dong June Yoon Do Hoon Kim Hyeon Ji Kim Chang Hyun Sung Han Sol Kang Chang Won Choi Seong Hwan Kim Young Shik Kim Hydrogen Peroxide- and Nitric Oxide-mediated Disease Control of Bacterial Wilt in Tomato Plants The Plant Pathology Journal antibacterial agents hydrogen peroxide nitric oxide Ralstonia solanacearum tomato bacterial wilt |
| title | Hydrogen Peroxide- and Nitric Oxide-mediated Disease Control of Bacterial Wilt in Tomato Plants |
| title_full | Hydrogen Peroxide- and Nitric Oxide-mediated Disease Control of Bacterial Wilt in Tomato Plants |
| title_fullStr | Hydrogen Peroxide- and Nitric Oxide-mediated Disease Control of Bacterial Wilt in Tomato Plants |
| title_full_unstemmed | Hydrogen Peroxide- and Nitric Oxide-mediated Disease Control of Bacterial Wilt in Tomato Plants |
| title_short | Hydrogen Peroxide- and Nitric Oxide-mediated Disease Control of Bacterial Wilt in Tomato Plants |
| title_sort | hydrogen peroxide and nitric oxide mediated disease control of bacterial wilt in tomato plants |
| topic | antibacterial agents hydrogen peroxide nitric oxide Ralstonia solanacearum tomato bacterial wilt |
| url | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174819/ |
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