Overexpression of <i>OsABCG48</i> Lowers Cadmium in Rice (<i>Oryza sativa</i> L.)
Cadmium pollution threatens food safety and security by causing health issues and reducing farmland availability. Engineering genetic changes in crop plants to lower Cd accumulation can be a cost-effective approach to address this problem. Previously, we reported that a rice line, 2B, which expresse...
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MDPI AG
2021-05-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/5/918 |
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| author | Xingzhe Cai Meng Wang Yucong Jiang Changhu Wang David W. Ow |
| author_facet | Xingzhe Cai Meng Wang Yucong Jiang Changhu Wang David W. Ow |
| author_sort | Xingzhe Cai |
| collection | DOAJ |
| description | Cadmium pollution threatens food safety and security by causing health issues and reducing farmland availability. Engineering genetic changes in crop plants to lower Cd accumulation can be a cost-effective approach to address this problem. Previously, we reported that a rice line, 2B, which expresses a truncated version of <i>OsO3L2</i> had reduced Cd accumulation throughout the plant, including in seed. However, downstream events caused by expression of this gene were not known. In this study, RNA-seq was used to identify differentially expressed genes between the wild type and 2B rice with or without Cd treatment, leading to the study of an <i>ABC</i> transporter gene, <i>OsABCG48</i> (ATP-Binding Cassette transporter G family member 48). Heterologous expression of <i>OsABCG48</i> conferred tolerance to Cd in <i>Schizosaccharomyces pombe</i>, <i>Arabidopsis</i> and rice. Moreover, overexpressing <i>OsABCG48</i> in rice lowered root Cd accumulation that was associated with more extensive lateral root development. These data suggest that <i>OsABCG48</i> might have applications for engineering low-Cd rice. |
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| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T11:38:41Z |
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| series | Agronomy |
| spelling | doaj.art-866d3d4948124a29b592c3d4768025f72023-11-21T18:40:22ZengMDPI AGAgronomy2073-43952021-05-0111591810.3390/agronomy11050918Overexpression of <i>OsABCG48</i> Lowers Cadmium in Rice (<i>Oryza sativa</i> L.)Xingzhe Cai0Meng Wang1Yucong Jiang2Changhu Wang3David W. Ow4Plant Gene Engineering Center, Chinese Academy of Sciences, Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Guangdong Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaPlant Gene Engineering Center, Chinese Academy of Sciences, Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Guangdong Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaPlant Gene Engineering Center, Chinese Academy of Sciences, Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Guangdong Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaPlant Gene Engineering Center, Chinese Academy of Sciences, Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Guangdong Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaPlant Gene Engineering Center, Chinese Academy of Sciences, Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Guangdong Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaCadmium pollution threatens food safety and security by causing health issues and reducing farmland availability. Engineering genetic changes in crop plants to lower Cd accumulation can be a cost-effective approach to address this problem. Previously, we reported that a rice line, 2B, which expresses a truncated version of <i>OsO3L2</i> had reduced Cd accumulation throughout the plant, including in seed. However, downstream events caused by expression of this gene were not known. In this study, RNA-seq was used to identify differentially expressed genes between the wild type and 2B rice with or without Cd treatment, leading to the study of an <i>ABC</i> transporter gene, <i>OsABCG48</i> (ATP-Binding Cassette transporter G family member 48). Heterologous expression of <i>OsABCG48</i> conferred tolerance to Cd in <i>Schizosaccharomyces pombe</i>, <i>Arabidopsis</i> and rice. Moreover, overexpressing <i>OsABCG48</i> in rice lowered root Cd accumulation that was associated with more extensive lateral root development. These data suggest that <i>OsABCG48</i> might have applications for engineering low-Cd rice.https://www.mdpi.com/2073-4395/11/5/918ABC transporterCd stressRNA-seqexpression profilingrice (<i>Oryza sativa</i>) |
| spellingShingle | Xingzhe Cai Meng Wang Yucong Jiang Changhu Wang David W. Ow Overexpression of <i>OsABCG48</i> Lowers Cadmium in Rice (<i>Oryza sativa</i> L.) Agronomy ABC transporter Cd stress RNA-seq expression profiling rice (<i>Oryza sativa</i>) |
| title | Overexpression of <i>OsABCG48</i> Lowers Cadmium in Rice (<i>Oryza sativa</i> L.) |
| title_full | Overexpression of <i>OsABCG48</i> Lowers Cadmium in Rice (<i>Oryza sativa</i> L.) |
| title_fullStr | Overexpression of <i>OsABCG48</i> Lowers Cadmium in Rice (<i>Oryza sativa</i> L.) |
| title_full_unstemmed | Overexpression of <i>OsABCG48</i> Lowers Cadmium in Rice (<i>Oryza sativa</i> L.) |
| title_short | Overexpression of <i>OsABCG48</i> Lowers Cadmium in Rice (<i>Oryza sativa</i> L.) |
| title_sort | overexpression of i osabcg48 i lowers cadmium in rice i oryza sativa i l |
| topic | ABC transporter Cd stress RNA-seq expression profiling rice (<i>Oryza sativa</i>) |
| url | https://www.mdpi.com/2073-4395/11/5/918 |
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