Population dynamics of two antilisterial cheese surface consortia revealed by temporal temperature gradient gel electrophoresis
<p>Abstract</p> <p>Background</p> <p>Surface contamination of smear cheese by <it>Listeria </it>spp. is of major concern for the industry. Complex smear ecosystems have been shown to harbor antilisterial potential but the microorganisms and mechanisms involv...
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| Format: | Article |
| Language: | English |
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BMC
2010-03-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/10/74 |
| _version_ | 1811286939169456128 |
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| author | Hasler Madlen Miescher Schwenninger Susanne Roth Emmanuelle Eugster-Meier Elisabeth Lacroix Christophe |
| author_facet | Hasler Madlen Miescher Schwenninger Susanne Roth Emmanuelle Eugster-Meier Elisabeth Lacroix Christophe |
| author_sort | Hasler Madlen |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Surface contamination of smear cheese by <it>Listeria </it>spp. is of major concern for the industry. Complex smear ecosystems have been shown to harbor antilisterial potential but the microorganisms and mechanisms involved in the inhibition mostly remain unclear, and are likely related to complex interactions than to production of single antimicrobial compounds. Bacterial biodiversity and population dynamics of complex smear ecosystems exhibiting antilisterial properties <it>in situ </it>were investigated by Temporal temperature gradient gel electrophoresis (TTGE), a culture independent technique, for two microbial consortia isolated from commercial Raclette type cheeses inoculated with defined commercial ripening cultures (F) or produced with an old-young smearing process (M).</p> <p>Results</p> <p>TTGE revealed nine bacterial species common to both F and M consortia, but consortium F exhibited a higher diversity than consortium M, with thirteen and ten species, respectively. Population dynamics were studied after application of the consortia on fresh-produced Raclette cheeses. TTGE analyses revealed a similar sequential development of the nine species common to both consortia. Beside common cheese surface bacteria (<it>Staphylococcus equorum, Corynebacterium </it>spp., <it>Brevibacterium linens, Microbacterium gubbeenense</it>, <it>Agrococcus casei</it>), the two consortia contained marine lactic acid bacteria (<it>Alkalibacterium kapii</it>, <it>Marinilactibacillus psychrotolerans</it>) that developed early in ripening (day 14 to 20), shortly after the growth of staphylococci (day 7). A decrease of <it>Listeria </it>counts was observed on cheese surface inoculated at day 7 with 0.1-1 × 10<sup>2 </sup>CFU cm<sup>-2</sup>, when cheeses were smeared with consortium F or M. <it>Listeria </it>counts went below the detection limit of the method between day 14 and 28 and no subsequent regrowth was detected over 60 to 80 ripening days. In contrast, <it>Listeria </it>grew to high counts (10<sup>5 </sup>CFU cm<sup>-2</sup>) on cheeses smeared with a defined surface culture.</p> <p>Conclusions</p> <p>This work reports the first population dynamics study of complex smear ecosystems exhibiting <it>in situ </it>antilisterial activity. TTGE revealed the presence of marine lactic acid bacteria that are likely related to the strong <it>Listeria </it>inhibition, as their early development in the smear occurred simultaneously with a decrease in <it>Listeria </it>cell count.</p> |
| first_indexed | 2024-04-13T03:08:51Z |
| format | Article |
| id | doaj.art-866fcdca9ac944648dca8fb96d700702 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-13T03:08:51Z |
| publishDate | 2010-03-01 |
| publisher | BMC |
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| series | BMC Microbiology |
| spelling | doaj.art-866fcdca9ac944648dca8fb96d7007022022-12-22T03:05:08ZengBMCBMC Microbiology1471-21802010-03-011017410.1186/1471-2180-10-74Population dynamics of two antilisterial cheese surface consortia revealed by temporal temperature gradient gel electrophoresisHasler MadlenMiescher Schwenninger SusanneRoth EmmanuelleEugster-Meier ElisabethLacroix Christophe<p>Abstract</p> <p>Background</p> <p>Surface contamination of smear cheese by <it>Listeria </it>spp. is of major concern for the industry. Complex smear ecosystems have been shown to harbor antilisterial potential but the microorganisms and mechanisms involved in the inhibition mostly remain unclear, and are likely related to complex interactions than to production of single antimicrobial compounds. Bacterial biodiversity and population dynamics of complex smear ecosystems exhibiting antilisterial properties <it>in situ </it>were investigated by Temporal temperature gradient gel electrophoresis (TTGE), a culture independent technique, for two microbial consortia isolated from commercial Raclette type cheeses inoculated with defined commercial ripening cultures (F) or produced with an old-young smearing process (M).</p> <p>Results</p> <p>TTGE revealed nine bacterial species common to both F and M consortia, but consortium F exhibited a higher diversity than consortium M, with thirteen and ten species, respectively. Population dynamics were studied after application of the consortia on fresh-produced Raclette cheeses. TTGE analyses revealed a similar sequential development of the nine species common to both consortia. Beside common cheese surface bacteria (<it>Staphylococcus equorum, Corynebacterium </it>spp., <it>Brevibacterium linens, Microbacterium gubbeenense</it>, <it>Agrococcus casei</it>), the two consortia contained marine lactic acid bacteria (<it>Alkalibacterium kapii</it>, <it>Marinilactibacillus psychrotolerans</it>) that developed early in ripening (day 14 to 20), shortly after the growth of staphylococci (day 7). A decrease of <it>Listeria </it>counts was observed on cheese surface inoculated at day 7 with 0.1-1 × 10<sup>2 </sup>CFU cm<sup>-2</sup>, when cheeses were smeared with consortium F or M. <it>Listeria </it>counts went below the detection limit of the method between day 14 and 28 and no subsequent regrowth was detected over 60 to 80 ripening days. In contrast, <it>Listeria </it>grew to high counts (10<sup>5 </sup>CFU cm<sup>-2</sup>) on cheeses smeared with a defined surface culture.</p> <p>Conclusions</p> <p>This work reports the first population dynamics study of complex smear ecosystems exhibiting <it>in situ </it>antilisterial activity. TTGE revealed the presence of marine lactic acid bacteria that are likely related to the strong <it>Listeria </it>inhibition, as their early development in the smear occurred simultaneously with a decrease in <it>Listeria </it>cell count.</p>http://www.biomedcentral.com/1471-2180/10/74 |
| spellingShingle | Hasler Madlen Miescher Schwenninger Susanne Roth Emmanuelle Eugster-Meier Elisabeth Lacroix Christophe Population dynamics of two antilisterial cheese surface consortia revealed by temporal temperature gradient gel electrophoresis BMC Microbiology |
| title | Population dynamics of two antilisterial cheese surface consortia revealed by temporal temperature gradient gel electrophoresis |
| title_full | Population dynamics of two antilisterial cheese surface consortia revealed by temporal temperature gradient gel electrophoresis |
| title_fullStr | Population dynamics of two antilisterial cheese surface consortia revealed by temporal temperature gradient gel electrophoresis |
| title_full_unstemmed | Population dynamics of two antilisterial cheese surface consortia revealed by temporal temperature gradient gel electrophoresis |
| title_short | Population dynamics of two antilisterial cheese surface consortia revealed by temporal temperature gradient gel electrophoresis |
| title_sort | population dynamics of two antilisterial cheese surface consortia revealed by temporal temperature gradient gel electrophoresis |
| url | http://www.biomedcentral.com/1471-2180/10/74 |
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