Ethanol- and PARP-Mediated Regulation of Ribosome-Associated Long Non-Coding RNA (lncRNA) in Pyramidal Neurons
Although, by definition, long noncoding RNAs (lncRNAs) are not translated, they are sometimes associated with ribosomes. In fact, some estimates suggest the existence of more than 50 K lncRNA molecules that could encode for small peptides. We examined the effects of an ethanol and Poly-ADP Ribose Po...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Non-Coding RNA |
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| Online Access: | https://www.mdpi.com/2311-553X/9/6/72 |
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| author | Hooriyah S. Rizavi Hannah E. Gavin Harish R. Krishnan David P. Gavin Rajiv P. Sharma |
| author_facet | Hooriyah S. Rizavi Hannah E. Gavin Harish R. Krishnan David P. Gavin Rajiv P. Sharma |
| author_sort | Hooriyah S. Rizavi |
| collection | DOAJ |
| description | Although, by definition, long noncoding RNAs (lncRNAs) are not translated, they are sometimes associated with ribosomes. In fact, some estimates suggest the existence of more than 50 K lncRNA molecules that could encode for small peptides. We examined the effects of an ethanol and Poly-ADP Ribose Polymerase (PARP) inhibitor (ABT-888) on ribosome-bound lncRNAs. Mice were administered via intraperitoneal injection (i.p.) either normal saline (CTL) or ethanol (EtOH) twice a day for four consecutive days. On the fourth day, a sub-group of mice administered with ethanol also received ABT-888 (EtOH+ABT). Ribosome-bound lncRNAs in CaMKIIα-expressing pyramidal neurons were measured using the Translating Ribosome Affinity Purification (TRAP) technique. Our findings show that EtOH altered the attachment of 107 lncRNA transcripts, while EtOH+ABT altered 60 lncRNAs. Among these 60 lncRNAs, 49 were altered by both conditions, while EtOH+ABT uniquely altered the attachment of 11 lncRNA transcripts that EtOH alone did not affect. To validate these results, we selected eight lncRNAs (Mir124-2hg, 5430416N02Rik, Snhg17, Snhg12, Snhg1, Mir9-3hg, Gas5, and 1110038B12Rik) for qRT-PCR analysis. The current study demonstrates that ethanol-induced changes in lncRNA attachment to ribosomes can be mitigated by the addition of the PARP inhibitor ABT-888. |
| first_indexed | 2024-03-08T20:29:05Z |
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| institution | Directory Open Access Journal |
| issn | 2311-553X |
| language | English |
| last_indexed | 2024-03-08T20:29:05Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Non-Coding RNA |
| spelling | doaj.art-8672879db4d34a2d95e840a918ecc5c32023-12-22T14:29:25ZengMDPI AGNon-Coding RNA2311-553X2023-11-01967210.3390/ncrna9060072Ethanol- and PARP-Mediated Regulation of Ribosome-Associated Long Non-Coding RNA (lncRNA) in Pyramidal NeuronsHooriyah S. Rizavi0Hannah E. Gavin1Harish R. Krishnan2David P. Gavin3Rajiv P. Sharma4Department of Psychiatry, University of Illinois at Chicago, Chicago, IL 60612, USADepartment of Psychiatry, University of Illinois at Chicago, Chicago, IL 60612, USACenter for Alcohol Research in Epigenetics, Department of Psychiatry, University of Illinois at Chicago, Chicago, IL 60612, USADepartment of Psychiatry, University of Illinois at Chicago, Chicago, IL 60612, USADepartment of Psychiatry, University of Illinois at Chicago, Chicago, IL 60612, USAAlthough, by definition, long noncoding RNAs (lncRNAs) are not translated, they are sometimes associated with ribosomes. In fact, some estimates suggest the existence of more than 50 K lncRNA molecules that could encode for small peptides. We examined the effects of an ethanol and Poly-ADP Ribose Polymerase (PARP) inhibitor (ABT-888) on ribosome-bound lncRNAs. Mice were administered via intraperitoneal injection (i.p.) either normal saline (CTL) or ethanol (EtOH) twice a day for four consecutive days. On the fourth day, a sub-group of mice administered with ethanol also received ABT-888 (EtOH+ABT). Ribosome-bound lncRNAs in CaMKIIα-expressing pyramidal neurons were measured using the Translating Ribosome Affinity Purification (TRAP) technique. Our findings show that EtOH altered the attachment of 107 lncRNA transcripts, while EtOH+ABT altered 60 lncRNAs. Among these 60 lncRNAs, 49 were altered by both conditions, while EtOH+ABT uniquely altered the attachment of 11 lncRNA transcripts that EtOH alone did not affect. To validate these results, we selected eight lncRNAs (Mir124-2hg, 5430416N02Rik, Snhg17, Snhg12, Snhg1, Mir9-3hg, Gas5, and 1110038B12Rik) for qRT-PCR analysis. The current study demonstrates that ethanol-induced changes in lncRNA attachment to ribosomes can be mitigated by the addition of the PARP inhibitor ABT-888.https://www.mdpi.com/2311-553X/9/6/72ribosome-bound lncRNAalcohol use disorderTRAPPARP inhibitorprefrontal cortexABT-888 |
| spellingShingle | Hooriyah S. Rizavi Hannah E. Gavin Harish R. Krishnan David P. Gavin Rajiv P. Sharma Ethanol- and PARP-Mediated Regulation of Ribosome-Associated Long Non-Coding RNA (lncRNA) in Pyramidal Neurons Non-Coding RNA ribosome-bound lncRNA alcohol use disorder TRAP PARP inhibitor prefrontal cortex ABT-888 |
| title | Ethanol- and PARP-Mediated Regulation of Ribosome-Associated Long Non-Coding RNA (lncRNA) in Pyramidal Neurons |
| title_full | Ethanol- and PARP-Mediated Regulation of Ribosome-Associated Long Non-Coding RNA (lncRNA) in Pyramidal Neurons |
| title_fullStr | Ethanol- and PARP-Mediated Regulation of Ribosome-Associated Long Non-Coding RNA (lncRNA) in Pyramidal Neurons |
| title_full_unstemmed | Ethanol- and PARP-Mediated Regulation of Ribosome-Associated Long Non-Coding RNA (lncRNA) in Pyramidal Neurons |
| title_short | Ethanol- and PARP-Mediated Regulation of Ribosome-Associated Long Non-Coding RNA (lncRNA) in Pyramidal Neurons |
| title_sort | ethanol and parp mediated regulation of ribosome associated long non coding rna lncrna in pyramidal neurons |
| topic | ribosome-bound lncRNA alcohol use disorder TRAP PARP inhibitor prefrontal cortex ABT-888 |
| url | https://www.mdpi.com/2311-553X/9/6/72 |
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