Interstitial Cells of Cajal and P<sub>2</sub>X<sub>3</sub> Receptors at Ureteropelvic Junction Obstruction and Their Relationship with Pain Response
<b>Introduction</b>: Etiopathogenesis and the symptomatology of ureteropelvic junction obstruction (UPJO) in the pediatric population has not yet been definitely clarified, suggesting a multifactorial nature of the condition. The aim was to analyze the association between the number of I...
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MDPI AG
2024-04-01
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| author | Dominika Borselle Maciej Kaczorowski Bartosz Gogolok Dariusz Patkowski Marcin Polok Agnieszka Hałoń Wojciech Apoznański |
| author_facet | Dominika Borselle Maciej Kaczorowski Bartosz Gogolok Dariusz Patkowski Marcin Polok Agnieszka Hałoń Wojciech Apoznański |
| author_sort | Dominika Borselle |
| collection | DOAJ |
| description | <b>Introduction</b>: Etiopathogenesis and the symptomatology of ureteropelvic junction obstruction (UPJO) in the pediatric population has not yet been definitely clarified, suggesting a multifactorial nature of the condition. The aim was to analyze the association between the number of Interstitial Cells of Cajal (ICCs), as well as P<sub>2</sub>X<sub>3</sub> receptors in ureteropelvic junction (UPJ) and the pain response in pediatric patients with hydronephrosis. <b>Methods:</b> 50 patients with congenital hydronephrosis underwent open or laparoscopic pyeloplasty at one of two departments of pediatric surgery and urology in Poland. Patients were divided into two groups according to the pain symptoms before surgery. A total of 50 samples of UPJ were obtained intraoperatively and underwent histopathological and immunohistochemical (IHC) analysis. Quantitative assessment of ICCs was based on the number of CD117(+) cells of adequate morphology in the subepithelial layer and the muscularis propria. Expression of P<sub>2</sub>X<sub>3</sub> receptors was evaluated as the intensity of IHC staining. <b>Results:</b> Patients with hydronephrosis and accompanying pain were on average 60 months older (77 vs. 17 months) than children with asymptomatic hydronephrosis (<i>p</i> = 0.017). Symptomatic children revealed higher numbers of ICCs in both the subepithelial layer and in the lamina muscularis propria. In particular, symptomatic patients aged 2 years or more exhibited significantly higher numbers of ICCs in the subepithelial layer. Significant differences in the distribution of ICCs between the subepithelial layer and the lamina muscularis propria were observed in both groups. Expression of P<sub>2</sub>X<sub>3</sub> receptors was limited to the urothelium and the muscle layer and correlated between these structures. There was no relationship between pain response and the expression of P<sub>2</sub>X<sub>3</sub> receptors. <b>Conclusions:</b> ICCs and P<sub>2</sub>X<sub>3</sub> receptors may participate in the pathogenesis of UPJO and in the modulation of pain response to a dilatation of the pyelocaliceal system. Explanation of the role of ICCs and P<sub>2</sub>X<sub>3</sub> receptors in propagation of ureteral peristaltic wave and the modulation of pain stimuli requires further studies. |
| first_indexed | 2024-04-24T10:41:20Z |
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| spelling | doaj.art-86741221a7b849a590ce3b8cef248ee12024-04-12T13:21:34ZengMDPI AGJournal of Clinical Medicine2077-03832024-04-01137210910.3390/jcm13072109Interstitial Cells of Cajal and P<sub>2</sub>X<sub>3</sub> Receptors at Ureteropelvic Junction Obstruction and Their Relationship with Pain ResponseDominika Borselle0Maciej Kaczorowski1Bartosz Gogolok2Dariusz Patkowski3Marcin Polok4Agnieszka Hałoń5Wojciech Apoznański6Department of Pediatric Surgery and Urology, Wroclaw Medical University and Hospital, Borowska 213, 50-556 Wroclaw, PolandDepartment of Clinical and Experimental Pathology, Wroclaw Medical University and Hospital, Borowska 213, 50-556 Wroclaw, PolandDepartment of Pediatric Surgery and Urology, Wroclaw Medical University and Hospital, Borowska 213, 50-556 Wroclaw, PolandDepartment of Pediatric Surgery and Urology, Wroclaw Medical University and Hospital, Borowska 213, 50-556 Wroclaw, PolandDepartment of Pediatric Surgery and Urology, Collegium Medicum—University of Zielona Gora, 65-417 Zielona Gora, PolandDepartment of Clinical and Experimental Pathology, Wroclaw Medical University and Hospital, Borowska 213, 50-556 Wroclaw, PolandDepartment of Pediatric Surgery and Urology, Wroclaw Medical University and Hospital, Borowska 213, 50-556 Wroclaw, Poland<b>Introduction</b>: Etiopathogenesis and the symptomatology of ureteropelvic junction obstruction (UPJO) in the pediatric population has not yet been definitely clarified, suggesting a multifactorial nature of the condition. The aim was to analyze the association between the number of Interstitial Cells of Cajal (ICCs), as well as P<sub>2</sub>X<sub>3</sub> receptors in ureteropelvic junction (UPJ) and the pain response in pediatric patients with hydronephrosis. <b>Methods:</b> 50 patients with congenital hydronephrosis underwent open or laparoscopic pyeloplasty at one of two departments of pediatric surgery and urology in Poland. Patients were divided into two groups according to the pain symptoms before surgery. A total of 50 samples of UPJ were obtained intraoperatively and underwent histopathological and immunohistochemical (IHC) analysis. Quantitative assessment of ICCs was based on the number of CD117(+) cells of adequate morphology in the subepithelial layer and the muscularis propria. Expression of P<sub>2</sub>X<sub>3</sub> receptors was evaluated as the intensity of IHC staining. <b>Results:</b> Patients with hydronephrosis and accompanying pain were on average 60 months older (77 vs. 17 months) than children with asymptomatic hydronephrosis (<i>p</i> = 0.017). Symptomatic children revealed higher numbers of ICCs in both the subepithelial layer and in the lamina muscularis propria. In particular, symptomatic patients aged 2 years or more exhibited significantly higher numbers of ICCs in the subepithelial layer. Significant differences in the distribution of ICCs between the subepithelial layer and the lamina muscularis propria were observed in both groups. Expression of P<sub>2</sub>X<sub>3</sub> receptors was limited to the urothelium and the muscle layer and correlated between these structures. There was no relationship between pain response and the expression of P<sub>2</sub>X<sub>3</sub> receptors. <b>Conclusions:</b> ICCs and P<sub>2</sub>X<sub>3</sub> receptors may participate in the pathogenesis of UPJO and in the modulation of pain response to a dilatation of the pyelocaliceal system. Explanation of the role of ICCs and P<sub>2</sub>X<sub>3</sub> receptors in propagation of ureteral peristaltic wave and the modulation of pain stimuli requires further studies.https://www.mdpi.com/2077-0383/13/7/2109hydronephrosispurinergic signalinginterstitial cells of Cajalpyeloplastyureteropelvic junction obstructioncongenital hydronephrosis |
| spellingShingle | Dominika Borselle Maciej Kaczorowski Bartosz Gogolok Dariusz Patkowski Marcin Polok Agnieszka Hałoń Wojciech Apoznański Interstitial Cells of Cajal and P<sub>2</sub>X<sub>3</sub> Receptors at Ureteropelvic Junction Obstruction and Their Relationship with Pain Response Journal of Clinical Medicine hydronephrosis purinergic signaling interstitial cells of Cajal pyeloplasty ureteropelvic junction obstruction congenital hydronephrosis |
| title | Interstitial Cells of Cajal and P<sub>2</sub>X<sub>3</sub> Receptors at Ureteropelvic Junction Obstruction and Their Relationship with Pain Response |
| title_full | Interstitial Cells of Cajal and P<sub>2</sub>X<sub>3</sub> Receptors at Ureteropelvic Junction Obstruction and Their Relationship with Pain Response |
| title_fullStr | Interstitial Cells of Cajal and P<sub>2</sub>X<sub>3</sub> Receptors at Ureteropelvic Junction Obstruction and Their Relationship with Pain Response |
| title_full_unstemmed | Interstitial Cells of Cajal and P<sub>2</sub>X<sub>3</sub> Receptors at Ureteropelvic Junction Obstruction and Their Relationship with Pain Response |
| title_short | Interstitial Cells of Cajal and P<sub>2</sub>X<sub>3</sub> Receptors at Ureteropelvic Junction Obstruction and Their Relationship with Pain Response |
| title_sort | interstitial cells of cajal and p sub 2 sub x sub 3 sub receptors at ureteropelvic junction obstruction and their relationship with pain response |
| topic | hydronephrosis purinergic signaling interstitial cells of Cajal pyeloplasty ureteropelvic junction obstruction congenital hydronephrosis |
| url | https://www.mdpi.com/2077-0383/13/7/2109 |
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