Plasma-Treated <i>Flammulina velutipes</i>-Derived Extract Showed Anticancer Potential in Human Breast Cancer Cells
Natural products with medicinal properties are among alternative therapies of interest due to their high body tolerance. We aimed to determine whether nonthermal gas plasma could enhance the medicinal value of <i>Flammulina velutipes</i> mushrooms. Generated gas plasma was characterized...
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MDPI AG
2020-11-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/23/8395 |
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| author | Sarmistha Mitra Pradeep Bhartiya Neha Kaushik Linh Nhat Nguyen Rizwan Wahab Sander Bekeschus Eun Ha Choi Nagendra Kumar Kaushik |
| author_facet | Sarmistha Mitra Pradeep Bhartiya Neha Kaushik Linh Nhat Nguyen Rizwan Wahab Sander Bekeschus Eun Ha Choi Nagendra Kumar Kaushik |
| author_sort | Sarmistha Mitra |
| collection | DOAJ |
| description | Natural products with medicinal properties are among alternative therapies of interest due to their high body tolerance. We aimed to determine whether nonthermal gas plasma could enhance the medicinal value of <i>Flammulina velutipes</i> mushrooms. Generated gas plasma was characterized by its emission spectrum in ambient air, pH, temperature, and H<sub>2</sub>O<sub>2</sub> and NO<sub>x</sub> concentrations after exposure for various periods. Phenolic and flavonoid contents in the extracts were measured using antioxidant assays and Fourier transform infrared and ultraviolet-visible spectroscopy. We analyzed the effects of the plasma-treated mushroom-derived extracts against breast carcinoma using the MCF7 and MDA-MB231 cell lines. The extracts significantly and concentration dependently inhibited the growth of breast cancer cells without inducing toxicity in normal MCF10A cells, and induced apoptosis via oxidative stress, evidenced by DNA damage (γ-H2AX foci formation), and increased the population of MCF7 breast cancer cells arrested in the G2/M phase of the cell cycle. The extracts also induced mitochondrion-mediated apoptosis of MCF7 cells through cytochrome c release and caspase cleavage activity. The plasma improved the biological activity of mushrooms by increasing their phenolic compounds that prevented the growth of breast cancer cells in vitro. |
| first_indexed | 2024-03-10T14:34:06Z |
| format | Article |
| id | doaj.art-867916ed770b4e47bd3a265b08d94f7e |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T14:34:06Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-867916ed770b4e47bd3a265b08d94f7e2023-11-20T22:20:33ZengMDPI AGApplied Sciences2076-34172020-11-011023839510.3390/app10238395Plasma-Treated <i>Flammulina velutipes</i>-Derived Extract Showed Anticancer Potential in Human Breast Cancer CellsSarmistha Mitra0Pradeep Bhartiya1Neha Kaushik2Linh Nhat Nguyen3Rizwan Wahab4Sander Bekeschus5Eun Ha Choi6Nagendra Kumar Kaushik7Plasma Bioscience Research Center/Applied Plasma Medicine Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, KoreaPlasma Bioscience Research Center/Applied Plasma Medicine Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, KoreaCollege of Engineering, Department of Biotechnology, University of Suwon, Hwaseong 18323, KoreaPlasma Bioscience Research Center/Applied Plasma Medicine Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, KoreaCollege of Science, Department of Zoology, King Saud University, Riyadh 11451, Saudi ArabiaCentre for Innovation Competence (ZIK) <i>Plasmatis</i>, Leibniz Institute for Plasma Science and Technology (INP Greifswald), 17489 Greifswald, GermanyPlasma Bioscience Research Center/Applied Plasma Medicine Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, KoreaPlasma Bioscience Research Center/Applied Plasma Medicine Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, KoreaNatural products with medicinal properties are among alternative therapies of interest due to their high body tolerance. We aimed to determine whether nonthermal gas plasma could enhance the medicinal value of <i>Flammulina velutipes</i> mushrooms. Generated gas plasma was characterized by its emission spectrum in ambient air, pH, temperature, and H<sub>2</sub>O<sub>2</sub> and NO<sub>x</sub> concentrations after exposure for various periods. Phenolic and flavonoid contents in the extracts were measured using antioxidant assays and Fourier transform infrared and ultraviolet-visible spectroscopy. We analyzed the effects of the plasma-treated mushroom-derived extracts against breast carcinoma using the MCF7 and MDA-MB231 cell lines. The extracts significantly and concentration dependently inhibited the growth of breast cancer cells without inducing toxicity in normal MCF10A cells, and induced apoptosis via oxidative stress, evidenced by DNA damage (γ-H2AX foci formation), and increased the population of MCF7 breast cancer cells arrested in the G2/M phase of the cell cycle. The extracts also induced mitochondrion-mediated apoptosis of MCF7 cells through cytochrome c release and caspase cleavage activity. The plasma improved the biological activity of mushrooms by increasing their phenolic compounds that prevented the growth of breast cancer cells in vitro.https://www.mdpi.com/2076-3417/10/23/8395gas plasmamushroomanticancercell death |
| spellingShingle | Sarmistha Mitra Pradeep Bhartiya Neha Kaushik Linh Nhat Nguyen Rizwan Wahab Sander Bekeschus Eun Ha Choi Nagendra Kumar Kaushik Plasma-Treated <i>Flammulina velutipes</i>-Derived Extract Showed Anticancer Potential in Human Breast Cancer Cells Applied Sciences gas plasma mushroom anticancer cell death |
| title | Plasma-Treated <i>Flammulina velutipes</i>-Derived Extract Showed Anticancer Potential in Human Breast Cancer Cells |
| title_full | Plasma-Treated <i>Flammulina velutipes</i>-Derived Extract Showed Anticancer Potential in Human Breast Cancer Cells |
| title_fullStr | Plasma-Treated <i>Flammulina velutipes</i>-Derived Extract Showed Anticancer Potential in Human Breast Cancer Cells |
| title_full_unstemmed | Plasma-Treated <i>Flammulina velutipes</i>-Derived Extract Showed Anticancer Potential in Human Breast Cancer Cells |
| title_short | Plasma-Treated <i>Flammulina velutipes</i>-Derived Extract Showed Anticancer Potential in Human Breast Cancer Cells |
| title_sort | plasma treated i flammulina velutipes i derived extract showed anticancer potential in human breast cancer cells |
| topic | gas plasma mushroom anticancer cell death |
| url | https://www.mdpi.com/2076-3417/10/23/8395 |
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