Global Diversity of Desert Hypolithic Cyanobacteria
Global patterns in diversity were estimated for cyanobacteria-dominated hypolithic communities that colonize ventral surfaces of quartz stones and are common in desert environments. A total of 64 hypolithic communities were recovered from deserts on every continent plus a tropical moisture sufficien...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00867/full |
| _version_ | 1819017903911993344 |
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| author | Donnabella C. Lacap-Bugler Kevin K. Lee Stephen Archer Len N. Gillman Maggie C.Y. Lau Sebastian Leuzinger Charles K. Lee Teruya Maki Christopher P. McKay John K. Perrott Asunción de los Rios-Murillo Kimberley A. Warren-Rhodes David W. Hopkins Stephen B. Pointing Stephen B. Pointing |
| author_facet | Donnabella C. Lacap-Bugler Kevin K. Lee Stephen Archer Len N. Gillman Maggie C.Y. Lau Sebastian Leuzinger Charles K. Lee Teruya Maki Christopher P. McKay John K. Perrott Asunción de los Rios-Murillo Kimberley A. Warren-Rhodes David W. Hopkins Stephen B. Pointing Stephen B. Pointing |
| author_sort | Donnabella C. Lacap-Bugler |
| collection | DOAJ |
| description | Global patterns in diversity were estimated for cyanobacteria-dominated hypolithic communities that colonize ventral surfaces of quartz stones and are common in desert environments. A total of 64 hypolithic communities were recovered from deserts on every continent plus a tropical moisture sufficient location. Community diversity was estimated using a combined t-RFLP fingerprinting and high throughput sequencing approach. The t-RFLP analysis revealed desert communities were different from the single non-desert location. A striking pattern also emerged where Antarctic desert communities were clearly distinct from all other deserts. Some overlap in community similarity occurred for hot, cold and tundra deserts. A further observation was that the producer-consumer ratio displayed a significant negative correlation with growing season, such that shorter growing seasons supported communities with greater abundance of producers, and this pattern was independent of macroclimate. High-throughput sequencing of 16S rRNA and nifH genes from four representative samples validated the t-RFLP study and revealed patterns of taxonomic and putative diazotrophic diversity for desert communities from the Taklimakan Desert, Tibetan Plateau, Canadian Arctic and Antarctic. All communities were dominated by cyanobacteria and among these 21 taxa were potentially endemic to any given desert location. Some others occurred in all but the most extreme hot and polar deserts suggesting they were relatively less well adapted to environmental stress. The t-RFLP and sequencing data revealed the two most abundant cyanobacterial taxa were Phormidium in Antarctic and Tibetan deserts and Chroococcidiopsis in hot and cold deserts. The Arctic tundra displayed a more heterogenous cyanobacterial assemblage and this was attributed to the maritime-influenced sampling location. The most abundant heterotrophic taxa were ubiquitous among samples and belonged to the Acidobacteria, Actinobacteria, Bacteroidetes, and Proteobacteria. Sequencing using nitrogenase gene-specific primers revealed all putative diazotrophs were Proteobacteria of the orders Burkholderiales, Rhizobiales, and Rhodospirillales. We envisage cyanobacterial carbon input to the system is accompanied by nitrogen fixation largely from non-cyanobacterial taxa. Overall the results indicate desert hypoliths worldwide are dominated by cyanobacteria and that growing season is a useful predictor of their abundance. Differences in cyanobacterial taxa encountered may reflect their adaptation to different moisture availability regimes in polar and non-polar deserts. |
| first_indexed | 2024-12-21T03:10:56Z |
| format | Article |
| id | doaj.art-86849e4baf87424d98c827db171be8f8 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-21T03:10:56Z |
| publishDate | 2017-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-86849e4baf87424d98c827db171be8f82022-12-21T19:17:58ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-05-01810.3389/fmicb.2017.00867248744Global Diversity of Desert Hypolithic CyanobacteriaDonnabella C. Lacap-Bugler0Kevin K. Lee1Stephen Archer2Len N. Gillman3Maggie C.Y. Lau4Sebastian Leuzinger5Charles K. Lee6Teruya Maki7Christopher P. McKay8John K. Perrott9Asunción de los Rios-Murillo10Kimberley A. Warren-Rhodes11David W. Hopkins12Stephen B. Pointing13Stephen B. Pointing14Institute for Applied Ecology New Zealand, School of Science, Auckland University of TechnologyAuckland, New ZealandInstitute for Applied Ecology New Zealand, School of Science, Auckland University of TechnologyAuckland, New ZealandInstitute for Applied Ecology New Zealand, School of Science, Auckland University of TechnologyAuckland, New ZealandInstitute for Applied Ecology New Zealand, School of Science, Auckland University of TechnologyAuckland, New ZealandDepartment of Geosciences, Princeton University, PrincetonNJ, USAInstitute for Applied Ecology New Zealand, School of Science, Auckland University of TechnologyAuckland, New ZealandInternational Centre for Terrestrial Antarctic Research, School of Science, University of WaikatoHamilton, New ZealandInstitute of Nature and Environmental Technology, Kanazawa UniversityKanazawa, JapanNational Aeronautics and Space Administration Ames Research Center, Moffett FieldCA, USAInstitute for Applied Ecology New Zealand, School of Science, Auckland University of TechnologyAuckland, New ZealandDepartamento de Biogeoquímica y Ecología Microbiana, Museo Nacional de Ciencias NaturalesMadrid, SpainInstitute of Nature and Environmental Technology, Kanazawa UniversityKanazawa, JapanThe Royal Agricultural UniversityCirencester, UKInstitute for Applied Ecology New Zealand, School of Science, Auckland University of TechnologyAuckland, New ZealandInstitute of Nature and Environmental Technology, Kanazawa UniversityKanazawa, JapanGlobal patterns in diversity were estimated for cyanobacteria-dominated hypolithic communities that colonize ventral surfaces of quartz stones and are common in desert environments. A total of 64 hypolithic communities were recovered from deserts on every continent plus a tropical moisture sufficient location. Community diversity was estimated using a combined t-RFLP fingerprinting and high throughput sequencing approach. The t-RFLP analysis revealed desert communities were different from the single non-desert location. A striking pattern also emerged where Antarctic desert communities were clearly distinct from all other deserts. Some overlap in community similarity occurred for hot, cold and tundra deserts. A further observation was that the producer-consumer ratio displayed a significant negative correlation with growing season, such that shorter growing seasons supported communities with greater abundance of producers, and this pattern was independent of macroclimate. High-throughput sequencing of 16S rRNA and nifH genes from four representative samples validated the t-RFLP study and revealed patterns of taxonomic and putative diazotrophic diversity for desert communities from the Taklimakan Desert, Tibetan Plateau, Canadian Arctic and Antarctic. All communities were dominated by cyanobacteria and among these 21 taxa were potentially endemic to any given desert location. Some others occurred in all but the most extreme hot and polar deserts suggesting they were relatively less well adapted to environmental stress. The t-RFLP and sequencing data revealed the two most abundant cyanobacterial taxa were Phormidium in Antarctic and Tibetan deserts and Chroococcidiopsis in hot and cold deserts. The Arctic tundra displayed a more heterogenous cyanobacterial assemblage and this was attributed to the maritime-influenced sampling location. The most abundant heterotrophic taxa were ubiquitous among samples and belonged to the Acidobacteria, Actinobacteria, Bacteroidetes, and Proteobacteria. Sequencing using nitrogenase gene-specific primers revealed all putative diazotrophs were Proteobacteria of the orders Burkholderiales, Rhizobiales, and Rhodospirillales. We envisage cyanobacterial carbon input to the system is accompanied by nitrogen fixation largely from non-cyanobacterial taxa. Overall the results indicate desert hypoliths worldwide are dominated by cyanobacteria and that growing season is a useful predictor of their abundance. Differences in cyanobacterial taxa encountered may reflect their adaptation to different moisture availability regimes in polar and non-polar deserts.http://journal.frontiersin.org/article/10.3389/fmicb.2017.00867/fullbiogeographycyanobacteriadesertdrylandhypolith |
| spellingShingle | Donnabella C. Lacap-Bugler Kevin K. Lee Stephen Archer Len N. Gillman Maggie C.Y. Lau Sebastian Leuzinger Charles K. Lee Teruya Maki Christopher P. McKay John K. Perrott Asunción de los Rios-Murillo Kimberley A. Warren-Rhodes David W. Hopkins Stephen B. Pointing Stephen B. Pointing Global Diversity of Desert Hypolithic Cyanobacteria Frontiers in Microbiology biogeography cyanobacteria desert dryland hypolith |
| title | Global Diversity of Desert Hypolithic Cyanobacteria |
| title_full | Global Diversity of Desert Hypolithic Cyanobacteria |
| title_fullStr | Global Diversity of Desert Hypolithic Cyanobacteria |
| title_full_unstemmed | Global Diversity of Desert Hypolithic Cyanobacteria |
| title_short | Global Diversity of Desert Hypolithic Cyanobacteria |
| title_sort | global diversity of desert hypolithic cyanobacteria |
| topic | biogeography cyanobacteria desert dryland hypolith |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00867/full |
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