Exploring Key Biomarkers and Common Pathogenesis of Seven Digestive System Cancers and Their Correlation with COVID-19
Digestive system cancer and COVID-19 significantly affect the digestive system, but the mechanism of interaction between COVID-19 and the digestive system cancers has not been fully elucidated. We downloaded the gene expression of COVID-19 and seven digestive system cancers (oral, esophageal, gastri...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/7/349 |
| _version_ | 1797589744040804352 |
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| author | Zuming Xiong Yongjun Yang Wenxin Li Yirong Lin Wei Huang Sen Zhang |
| author_facet | Zuming Xiong Yongjun Yang Wenxin Li Yirong Lin Wei Huang Sen Zhang |
| author_sort | Zuming Xiong |
| collection | DOAJ |
| description | Digestive system cancer and COVID-19 significantly affect the digestive system, but the mechanism of interaction between COVID-19 and the digestive system cancers has not been fully elucidated. We downloaded the gene expression of COVID-19 and seven digestive system cancers (oral, esophageal, gastric, colorectal, hepatocellular, bile duct, pancreatic) from GEO and identified hub differentially expressed genes. Multiple verifications, diagnostic efficacy, prognostic analysis, functional enrichment and related transcription factors of hub genes were explored. We identified 23 common DEGs for subsequent analysis. CytoHubba identified nine hub genes (<i>CCNA2</i>, <i>CCNB1</i>, <i>CDKN3</i>, <i>ECT2</i>, <i>KIF14</i>, <i>KIF20A</i>, <i>KIF4A</i>, <i>NEK2</i>, <i>TTK</i>). TCGA and GEO data validated the expression and excellent diagnostic and prognostic ability of hub genes. Functional analysis revealed that the processes of cell division and the cell cycle were essential in COVID-19 and digestive system cancers. Furthermore, six related transcription factors (E2F1, E2F3, E2F4, MYC, TP53, YBX1) were involved in hub gene regulation. Via in vitro experiments, <i>CCNA2</i>, <i>CCNB1</i>, and <i>MYC</i> expression was verified in 25 colorectal cancer tissue pairs. Our study revealed the key biomarks and common pathogenesis of digestive system cancers and COVID-19. These may provide new ideas for further mechanistic research. |
| first_indexed | 2024-03-11T01:11:47Z |
| format | Article |
| id | doaj.art-8684a86adbe645f3b3ae3106158ac1af |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-11T01:11:47Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-8684a86adbe645f3b3ae3106158ac1af2023-11-18T18:50:59ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-06-014575515553310.3390/cimb45070349Exploring Key Biomarkers and Common Pathogenesis of Seven Digestive System Cancers and Their Correlation with COVID-19Zuming Xiong0Yongjun Yang1Wenxin Li2Yirong Lin3Wei Huang4Sen Zhang5Department of Colorectal and Anal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, ChinaDepartment of Colorectal and Anal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, ChinaDepartment of Colorectal and Anal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, ChinaDepartment of Colorectal and Anal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, ChinaDepartment of Colorectal and Anal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, ChinaDepartment of Colorectal and Anal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, ChinaDigestive system cancer and COVID-19 significantly affect the digestive system, but the mechanism of interaction between COVID-19 and the digestive system cancers has not been fully elucidated. We downloaded the gene expression of COVID-19 and seven digestive system cancers (oral, esophageal, gastric, colorectal, hepatocellular, bile duct, pancreatic) from GEO and identified hub differentially expressed genes. Multiple verifications, diagnostic efficacy, prognostic analysis, functional enrichment and related transcription factors of hub genes were explored. We identified 23 common DEGs for subsequent analysis. CytoHubba identified nine hub genes (<i>CCNA2</i>, <i>CCNB1</i>, <i>CDKN3</i>, <i>ECT2</i>, <i>KIF14</i>, <i>KIF20A</i>, <i>KIF4A</i>, <i>NEK2</i>, <i>TTK</i>). TCGA and GEO data validated the expression and excellent diagnostic and prognostic ability of hub genes. Functional analysis revealed that the processes of cell division and the cell cycle were essential in COVID-19 and digestive system cancers. Furthermore, six related transcription factors (E2F1, E2F3, E2F4, MYC, TP53, YBX1) were involved in hub gene regulation. Via in vitro experiments, <i>CCNA2</i>, <i>CCNB1</i>, and <i>MYC</i> expression was verified in 25 colorectal cancer tissue pairs. Our study revealed the key biomarks and common pathogenesis of digestive system cancers and COVID-19. These may provide new ideas for further mechanistic research.https://www.mdpi.com/1467-3045/45/7/349digestive system cancersCOVID-19common biomarkerspathogenesisdiagnosisprognosis |
| spellingShingle | Zuming Xiong Yongjun Yang Wenxin Li Yirong Lin Wei Huang Sen Zhang Exploring Key Biomarkers and Common Pathogenesis of Seven Digestive System Cancers and Their Correlation with COVID-19 Current Issues in Molecular Biology digestive system cancers COVID-19 common biomarkers pathogenesis diagnosis prognosis |
| title | Exploring Key Biomarkers and Common Pathogenesis of Seven Digestive System Cancers and Their Correlation with COVID-19 |
| title_full | Exploring Key Biomarkers and Common Pathogenesis of Seven Digestive System Cancers and Their Correlation with COVID-19 |
| title_fullStr | Exploring Key Biomarkers and Common Pathogenesis of Seven Digestive System Cancers and Their Correlation with COVID-19 |
| title_full_unstemmed | Exploring Key Biomarkers and Common Pathogenesis of Seven Digestive System Cancers and Their Correlation with COVID-19 |
| title_short | Exploring Key Biomarkers and Common Pathogenesis of Seven Digestive System Cancers and Their Correlation with COVID-19 |
| title_sort | exploring key biomarkers and common pathogenesis of seven digestive system cancers and their correlation with covid 19 |
| topic | digestive system cancers COVID-19 common biomarkers pathogenesis diagnosis prognosis |
| url | https://www.mdpi.com/1467-3045/45/7/349 |
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