Tephrosia toxicaria (Sw.) Pers. extracts: Screening by examining aedicidal action under laboratory and field conditions along with its antioxidant, antileishmanial, and antimicrobial activities
An increase in the incidence of arboviral, microbial and parasitic infections, and to disorders related to oxidative stress has encouraged the development of adjuvant therapies based on natural formulations, such as those involving plant extracts. Thus, to expand the repertoire of the available ther...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9833590/?tool=EBI |
| _version_ | 1797952791710269440 |
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| author | Giulian César da Silva Sá Leidiane Barboza da Silva Pedro Vitor Vale Bezerra Melissa Alves Farias da Silva Cássio Lázaro Silva Inacio Weslley de Souza Paiva Virgínia Penéllope Macedo e Silva Laísa Vilar Cordeiro Johny Wysllas de Freitas Oliveira Marcelo Sousa Silva Edeltrudes de Oliveira Lima Francisco José Carvalho Moreira Hugo Alexandre de Oliveira Rocha Patricia Batista Barra Maria de Fátima Freire de Melo Ximenes Adriana Ferreira Uchôa |
| author_facet | Giulian César da Silva Sá Leidiane Barboza da Silva Pedro Vitor Vale Bezerra Melissa Alves Farias da Silva Cássio Lázaro Silva Inacio Weslley de Souza Paiva Virgínia Penéllope Macedo e Silva Laísa Vilar Cordeiro Johny Wysllas de Freitas Oliveira Marcelo Sousa Silva Edeltrudes de Oliveira Lima Francisco José Carvalho Moreira Hugo Alexandre de Oliveira Rocha Patricia Batista Barra Maria de Fátima Freire de Melo Ximenes Adriana Ferreira Uchôa |
| author_sort | Giulian César da Silva Sá |
| collection | DOAJ |
| description | An increase in the incidence of arboviral, microbial and parasitic infections, and to disorders related to oxidative stress has encouraged the development of adjuvant therapies based on natural formulations, such as those involving plant extracts. Thus, to expand the repertoire of the available therapeutic options, this study aimed to describe the versatility of Tephrosia toxicaria (Sw.) (Pers., 1807) extracts for the control of arbovirus vectors, as well as their antioxidant, antileishmanial, and antimicrobial potential. Among the aqueous and hydroethanolic extracts obtained, the hydroethanolic extract from roots (RHA) was identified as the most active larvicide extract demonstrating, respectively, the lowest lethal concentration (mg/mL) for 50%, 90% and 99% of Aedes aegypti (L., 1762) and Aedes albopictus (S., 1894) larvae, observed at 24 h (0.33, 0.84 and 1.80; 0.32, 0.70 and 1.32) and 48 h (0.17, 0.51 and 1.22; 0.26, 0.47 and 0.78) post-exposure. Field assays revealed that RHA (0.84 mg/mL) is a potential oviposition deterrent, reducing egg-laying by approximately 90%. RHA (0.1 mg/mL) also exhibited antioxidant activity for the following tests: total antioxidant capacity (286.86 mg AAE/g), iron (87.16%) and copper (25.64%) chelation, and superoxide scavenging (10%). In the cell culture assays, RHA (0.1 mg/mL) promoted regeneration of metabolic activity (92% cell viability) in cells exposed to oxidative stress. Furthermore, RHA displayed weak antileishmanial activity (IC50 = 3.53 mg/mL) against Leishmania amazonensis and not exhibit antimicrobial activity. The extraction favored the concentration of carbohydrates in RHA, in addition to lectins and protease inhibitors, with molecular masses estimated between 10 and 24 kDa. Cytotoxicity and phytotoxicity analyses of RHA suggested its biosecurity. Thus, RHA is a multivalent extract with insecticide and antioxidant properties at low and safe concentrations. However, others studies on its indirect toxic effects are ongoing to ensure the complete safety of RHA. |
| first_indexed | 2024-04-10T22:51:41Z |
| format | Article |
| id | doaj.art-8685da1072a044c0a4e6cf7845d44be8 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-10T22:51:41Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8685da1072a044c0a4e6cf7845d44be82023-01-15T05:31:11ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01181Tephrosia toxicaria (Sw.) Pers. extracts: Screening by examining aedicidal action under laboratory and field conditions along with its antioxidant, antileishmanial, and antimicrobial activitiesGiulian César da Silva SáLeidiane Barboza da SilvaPedro Vitor Vale BezerraMelissa Alves Farias da SilvaCássio Lázaro Silva InacioWeslley de Souza PaivaVirgínia Penéllope Macedo e SilvaLaísa Vilar CordeiroJohny Wysllas de Freitas OliveiraMarcelo Sousa SilvaEdeltrudes de Oliveira LimaFrancisco José Carvalho MoreiraHugo Alexandre de Oliveira RochaPatricia Batista BarraMaria de Fátima Freire de Melo XimenesAdriana Ferreira UchôaAn increase in the incidence of arboviral, microbial and parasitic infections, and to disorders related to oxidative stress has encouraged the development of adjuvant therapies based on natural formulations, such as those involving plant extracts. Thus, to expand the repertoire of the available therapeutic options, this study aimed to describe the versatility of Tephrosia toxicaria (Sw.) (Pers., 1807) extracts for the control of arbovirus vectors, as well as their antioxidant, antileishmanial, and antimicrobial potential. Among the aqueous and hydroethanolic extracts obtained, the hydroethanolic extract from roots (RHA) was identified as the most active larvicide extract demonstrating, respectively, the lowest lethal concentration (mg/mL) for 50%, 90% and 99% of Aedes aegypti (L., 1762) and Aedes albopictus (S., 1894) larvae, observed at 24 h (0.33, 0.84 and 1.80; 0.32, 0.70 and 1.32) and 48 h (0.17, 0.51 and 1.22; 0.26, 0.47 and 0.78) post-exposure. Field assays revealed that RHA (0.84 mg/mL) is a potential oviposition deterrent, reducing egg-laying by approximately 90%. RHA (0.1 mg/mL) also exhibited antioxidant activity for the following tests: total antioxidant capacity (286.86 mg AAE/g), iron (87.16%) and copper (25.64%) chelation, and superoxide scavenging (10%). In the cell culture assays, RHA (0.1 mg/mL) promoted regeneration of metabolic activity (92% cell viability) in cells exposed to oxidative stress. Furthermore, RHA displayed weak antileishmanial activity (IC50 = 3.53 mg/mL) against Leishmania amazonensis and not exhibit antimicrobial activity. The extraction favored the concentration of carbohydrates in RHA, in addition to lectins and protease inhibitors, with molecular masses estimated between 10 and 24 kDa. Cytotoxicity and phytotoxicity analyses of RHA suggested its biosecurity. Thus, RHA is a multivalent extract with insecticide and antioxidant properties at low and safe concentrations. However, others studies on its indirect toxic effects are ongoing to ensure the complete safety of RHA.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9833590/?tool=EBI |
| spellingShingle | Giulian César da Silva Sá Leidiane Barboza da Silva Pedro Vitor Vale Bezerra Melissa Alves Farias da Silva Cássio Lázaro Silva Inacio Weslley de Souza Paiva Virgínia Penéllope Macedo e Silva Laísa Vilar Cordeiro Johny Wysllas de Freitas Oliveira Marcelo Sousa Silva Edeltrudes de Oliveira Lima Francisco José Carvalho Moreira Hugo Alexandre de Oliveira Rocha Patricia Batista Barra Maria de Fátima Freire de Melo Ximenes Adriana Ferreira Uchôa Tephrosia toxicaria (Sw.) Pers. extracts: Screening by examining aedicidal action under laboratory and field conditions along with its antioxidant, antileishmanial, and antimicrobial activities PLoS ONE |
| title | Tephrosia toxicaria (Sw.) Pers. extracts: Screening by examining aedicidal action under laboratory and field conditions along with its antioxidant, antileishmanial, and antimicrobial activities |
| title_full | Tephrosia toxicaria (Sw.) Pers. extracts: Screening by examining aedicidal action under laboratory and field conditions along with its antioxidant, antileishmanial, and antimicrobial activities |
| title_fullStr | Tephrosia toxicaria (Sw.) Pers. extracts: Screening by examining aedicidal action under laboratory and field conditions along with its antioxidant, antileishmanial, and antimicrobial activities |
| title_full_unstemmed | Tephrosia toxicaria (Sw.) Pers. extracts: Screening by examining aedicidal action under laboratory and field conditions along with its antioxidant, antileishmanial, and antimicrobial activities |
| title_short | Tephrosia toxicaria (Sw.) Pers. extracts: Screening by examining aedicidal action under laboratory and field conditions along with its antioxidant, antileishmanial, and antimicrobial activities |
| title_sort | tephrosia toxicaria sw pers extracts screening by examining aedicidal action under laboratory and field conditions along with its antioxidant antileishmanial and antimicrobial activities |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9833590/?tool=EBI |
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