Functional identification of MdMYB5 involved in secondary cell wall formation in apple
Being the principal elements of secondary cell wall, cellulose and lignin both play a strengthening role in plant structures and stress resistance. However, little research has been done regarding the molecular mechanisms involved in the formation of cellulose and lignin in apple. In this study, in...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Maximum Academic Press
2021-01-01
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| Series: | Fruit Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/FruRes-2021-0006 |
| _version_ | 1797293173301575680 |
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| author | Keqin Chen Xiaoguang Tang Mengru Song Yunna Guo Lifu Liu Hao Xue Hongyan Dai Zhihong Zhang |
| author_facet | Keqin Chen Xiaoguang Tang Mengru Song Yunna Guo Lifu Liu Hao Xue Hongyan Dai Zhihong Zhang |
| author_sort | Keqin Chen |
| collection | DOAJ |
| description | Being the principal elements of secondary cell wall, cellulose and lignin both play a strengthening role in plant structures and stress resistance. However, little research has been done regarding the molecular mechanisms involved in the formation of cellulose and lignin in apple. In this study, in order to better understand the regulatory network in the formation of secondary cell wall, an R2R3 MYB transcriptional factor MdMYB5 was identified and explored. The subcellular localization experiments showed that MdMYB5 could function in the nucleus. Even though lignin and cellulose content, and the expression of their biosynthesis related genes decreased in the MdMYB5-RNAi apple, the ectopic overexpression of MdMYB5 promotes lignin and cellulose content in Arabidopsis, which contributes to the dwarf phenotype. At the same time, salt and osmotic stress affect MdMYB5-RNAi apple tissue cultures. Further transcriptional activation assays carried out demonstrated that MdMYB5 could be activated by MdMYB46 and MdSND1. In conclusion, MdMYB5 was required for the normal formation of secondary cell wall in apple and could be activated by the key regulatory factors MdMYB46 and MdSND1. |
| first_indexed | 2024-03-07T20:09:02Z |
| format | Article |
| id | doaj.art-868995358c864cd38dabde93624762d2 |
| institution | Directory Open Access Journal |
| issn | 2769-4615 |
| language | English |
| last_indexed | 2024-03-07T20:09:02Z |
| publishDate | 2021-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Fruit Research |
| spelling | doaj.art-868995358c864cd38dabde93624762d22024-02-28T03:19:23ZengMaximum Academic PressFruit Research2769-46152021-01-011111010.48130/FruRes-2021-0006FruRes-2021-0006Functional identification of MdMYB5 involved in secondary cell wall formation in appleKeqin Chen0Xiaoguang Tang1Mengru Song2Yunna Guo3Lifu Liu4Hao Xue5Hongyan Dai6Zhihong Zhang7Group of Molecular Biology of Fruit Trees, College of Horticulture, Shenyang Agricultural University, 120 Dongling Road, Shenyang, Liaoning 110866, ChinaGroup of Molecular Biology of Fruit Trees, College of Horticulture, Shenyang Agricultural University, 120 Dongling Road, Shenyang, Liaoning 110866, ChinaGroup of Fruit Germplasm Evaluation & Utilization, College of Horticulture, Shenyang Agricultural University, 120 Dongling Road, Shenyang, Liaoning 110866, ChinaGroup of Fruit Germplasm Evaluation & Utilization, College of Horticulture, Shenyang Agricultural University, 120 Dongling Road, Shenyang, Liaoning 110866, ChinaGroup of Fruit Germplasm Evaluation & Utilization, College of Horticulture, Shenyang Agricultural University, 120 Dongling Road, Shenyang, Liaoning 110866, ChinaGroup of Molecular Biology of Fruit Trees, College of Horticulture, Shenyang Agricultural University, 120 Dongling Road, Shenyang, Liaoning 110866, ChinaGroup of Fruit Germplasm Evaluation & Utilization, College of Horticulture, Shenyang Agricultural University, 120 Dongling Road, Shenyang, Liaoning 110866, ChinaGroup of Molecular Biology of Fruit Trees, College of Horticulture, Shenyang Agricultural University, 120 Dongling Road, Shenyang, Liaoning 110866, ChinaBeing the principal elements of secondary cell wall, cellulose and lignin both play a strengthening role in plant structures and stress resistance. However, little research has been done regarding the molecular mechanisms involved in the formation of cellulose and lignin in apple. In this study, in order to better understand the regulatory network in the formation of secondary cell wall, an R2R3 MYB transcriptional factor MdMYB5 was identified and explored. The subcellular localization experiments showed that MdMYB5 could function in the nucleus. Even though lignin and cellulose content, and the expression of their biosynthesis related genes decreased in the MdMYB5-RNAi apple, the ectopic overexpression of MdMYB5 promotes lignin and cellulose content in Arabidopsis, which contributes to the dwarf phenotype. At the same time, salt and osmotic stress affect MdMYB5-RNAi apple tissue cultures. Further transcriptional activation assays carried out demonstrated that MdMYB5 could be activated by MdMYB46 and MdSND1. In conclusion, MdMYB5 was required for the normal formation of secondary cell wall in apple and could be activated by the key regulatory factors MdMYB46 and MdSND1.https://www.maxapress.com/article/doi/10.48130/FruRes-2021-0006applemdmyb5celluloseligninresistance to salt and osmotic stress |
| spellingShingle | Keqin Chen Xiaoguang Tang Mengru Song Yunna Guo Lifu Liu Hao Xue Hongyan Dai Zhihong Zhang Functional identification of MdMYB5 involved in secondary cell wall formation in apple Fruit Research apple mdmyb5 cellulose lignin resistance to salt and osmotic stress |
| title | Functional identification of MdMYB5 involved in secondary cell wall formation in apple |
| title_full | Functional identification of MdMYB5 involved in secondary cell wall formation in apple |
| title_fullStr | Functional identification of MdMYB5 involved in secondary cell wall formation in apple |
| title_full_unstemmed | Functional identification of MdMYB5 involved in secondary cell wall formation in apple |
| title_short | Functional identification of MdMYB5 involved in secondary cell wall formation in apple |
| title_sort | functional identification of mdmyb5 involved in secondary cell wall formation in apple |
| topic | apple mdmyb5 cellulose lignin resistance to salt and osmotic stress |
| url | https://www.maxapress.com/article/doi/10.48130/FruRes-2021-0006 |
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