Strategy for enhancing Cr(VI)-contaminated soil remediation and safe utilization by microbial-humic acid-vermiculite-alginate immobilized biocomposite
Bioreduction is an efficient approach to in-situ remediate Cr(VI)-contaminated soil, but further strengthening methods are still urgently needed. Herein, a novel immobilized biocomposite (B-HA-VE-SA) was successfully synthesized by embedding a efficient strain Bacillus sp. CRB-7 with humic acid (HA)...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322007965 |
| _version_ | 1798004512196132864 |
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| author | Minghui Wu Qiqi Wang Can Wang Qilu Zeng Jianpeng Li Han Wu Bin Wu Heng Xu Zhongping Qiu |
| author_facet | Minghui Wu Qiqi Wang Can Wang Qilu Zeng Jianpeng Li Han Wu Bin Wu Heng Xu Zhongping Qiu |
| author_sort | Minghui Wu |
| collection | DOAJ |
| description | Bioreduction is an efficient approach to in-situ remediate Cr(VI)-contaminated soil, but further strengthening methods are still urgently needed. Herein, a novel immobilized biocomposite (B-HA-VE-SA) was successfully synthesized by embedding a efficient strain Bacillus sp. CRB-7 with humic acid (HA) combined vermiculite (VE) and sodium alginate (SA). The performance and enhancement mechanism of the immobilized biocomposite on remediating Cr(VI)-contaminated soil were also investigated by analyzing the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response. Genomic annotation demonstrated that CRB-7 contains multiple genes contributed to Cr(VI) tolerance, Cr(VI) reduction and other metals resistance. Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process. Furthermore, the synergies of CRB-7 and immobilizers (HA, VE and SA) significantly improved soil microecology (soil enzyme activities, microbial quantity and diversity), and engendered the evolution of microbial community composition and functional pathways. Consequently, pot experiments (Brassica napus L.) verified the plant-growth-promoting (12.00–18.00% and 43.82–69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects (19.47–91.09% and 29.11–89.80% lower in root and aerial parts) of free and immobilized CRB-7. Taken together, these findings highlighted the superiority of B-HA-VE-SA in simultaneous remediation, microecological improvement and safe utilization of Cr(VI)-contaminated soil. |
| first_indexed | 2024-04-11T12:25:53Z |
| format | Article |
| id | doaj.art-868d1aaf749c4b86a5ac81b1b9ef2177 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-11T12:25:53Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-868d1aaf749c4b86a5ac81b1b9ef21772022-12-22T04:23:57ZengElsevierEcotoxicology and Environmental Safety0147-65132022-09-01243113956Strategy for enhancing Cr(VI)-contaminated soil remediation and safe utilization by microbial-humic acid-vermiculite-alginate immobilized biocompositeMinghui Wu0Qiqi Wang1Can Wang2Qilu Zeng3Jianpeng Li4Han Wu5Bin Wu6Heng Xu7Zhongping Qiu8School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, Sichuan, PR ChinaSchool of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, Sichuan, PR ChinaSchool of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, Sichuan, PR ChinaSchool of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, Sichuan, PR ChinaSchool of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, Sichuan, PR ChinaSchool of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, Sichuan, PR ChinaCollege of Ecology and Environment, Chengdu University of Technology, 1# Dongsanlu, Erxianqiao, Chengdu 610059, Sichuan, PR ChinaKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, Sichuan, PR China; Correspondence to: No. 111 Second Ring Road, Chengdu, Sichuan 610031, PR China.School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, Sichuan, PR China; Correspondence to: No. 111 Second Ring Road, Chengdu, Sichuan 610031, PR China.Bioreduction is an efficient approach to in-situ remediate Cr(VI)-contaminated soil, but further strengthening methods are still urgently needed. Herein, a novel immobilized biocomposite (B-HA-VE-SA) was successfully synthesized by embedding a efficient strain Bacillus sp. CRB-7 with humic acid (HA) combined vermiculite (VE) and sodium alginate (SA). The performance and enhancement mechanism of the immobilized biocomposite on remediating Cr(VI)-contaminated soil were also investigated by analyzing the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response. Genomic annotation demonstrated that CRB-7 contains multiple genes contributed to Cr(VI) tolerance, Cr(VI) reduction and other metals resistance. Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr(VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process. Furthermore, the synergies of CRB-7 and immobilizers (HA, VE and SA) significantly improved soil microecology (soil enzyme activities, microbial quantity and diversity), and engendered the evolution of microbial community composition and functional pathways. Consequently, pot experiments (Brassica napus L.) verified the plant-growth-promoting (12.00–18.00% and 43.82–69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects (19.47–91.09% and 29.11–89.80% lower in root and aerial parts) of free and immobilized CRB-7. Taken together, these findings highlighted the superiority of B-HA-VE-SA in simultaneous remediation, microecological improvement and safe utilization of Cr(VI)-contaminated soil.http://www.sciencedirect.com/science/article/pii/S0147651322007965Cr(VI)-reducing bacteria CRB-7Immobilized biocompositeWhole genomeHumic acidVermiculiteSoil micro-ecology |
| spellingShingle | Minghui Wu Qiqi Wang Can Wang Qilu Zeng Jianpeng Li Han Wu Bin Wu Heng Xu Zhongping Qiu Strategy for enhancing Cr(VI)-contaminated soil remediation and safe utilization by microbial-humic acid-vermiculite-alginate immobilized biocomposite Ecotoxicology and Environmental Safety Cr(VI)-reducing bacteria CRB-7 Immobilized biocomposite Whole genome Humic acid Vermiculite Soil micro-ecology |
| title | Strategy for enhancing Cr(VI)-contaminated soil remediation and safe utilization by microbial-humic acid-vermiculite-alginate immobilized biocomposite |
| title_full | Strategy for enhancing Cr(VI)-contaminated soil remediation and safe utilization by microbial-humic acid-vermiculite-alginate immobilized biocomposite |
| title_fullStr | Strategy for enhancing Cr(VI)-contaminated soil remediation and safe utilization by microbial-humic acid-vermiculite-alginate immobilized biocomposite |
| title_full_unstemmed | Strategy for enhancing Cr(VI)-contaminated soil remediation and safe utilization by microbial-humic acid-vermiculite-alginate immobilized biocomposite |
| title_short | Strategy for enhancing Cr(VI)-contaminated soil remediation and safe utilization by microbial-humic acid-vermiculite-alginate immobilized biocomposite |
| title_sort | strategy for enhancing cr vi contaminated soil remediation and safe utilization by microbial humic acid vermiculite alginate immobilized biocomposite |
| topic | Cr(VI)-reducing bacteria CRB-7 Immobilized biocomposite Whole genome Humic acid Vermiculite Soil micro-ecology |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322007965 |
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