Differentiation Induction of Mesenchymal Stem Cells by a Au Delivery Platform
Au decorated with type I collagen (Col) was used as a core material to cross-link with stromal cell-derived factor 1α (SDF1α) in order to investigate biological performance. The Au-based nanoparticles were subjected to physicochemical determination using scanning electron microscopy (SEM), dynamic l...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/12/14/1893 |
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| author | Meng-Yin Yang Cheng-Di Chiu Yi-Chun Ke Yi-Chin Yang Kai-Bo Chang Chien-Min Chen Hsu-Tung Lee Chien-Lun Tang Bai-Shuan Liu Huey-Shan Hung |
| author_facet | Meng-Yin Yang Cheng-Di Chiu Yi-Chun Ke Yi-Chin Yang Kai-Bo Chang Chien-Min Chen Hsu-Tung Lee Chien-Lun Tang Bai-Shuan Liu Huey-Shan Hung |
| author_sort | Meng-Yin Yang |
| collection | DOAJ |
| description | Au decorated with type I collagen (Col) was used as a core material to cross-link with stromal cell-derived factor 1α (SDF1α) in order to investigate biological performance. The Au-based nanoparticles were subjected to physicochemical determination using scanning electron microscopy (SEM), dynamic light scattering (DLS) and ultraviolet–visible (UV-Vis) and Fourier-transform infrared spectroscopy (FTIR). Mesenchymal stem cells (MSCs) were used to evaluate the biocompatibility of this nanoparticle using the MTT assay and measuring reactive oxygen species (ROS) production. Also, the biological effects of the SDF-1α-conjugated nanoparticles (Au-Col-SDF1α) were assessed and the mechanisms were explored. Furthermore, we investigated the cell differentiation-inducing potential of these conjugated nanoparticles on MSCs toward endothelial cells, neurons, osteoblasts and adipocytes. We then ultimately explored the process of cell entry and transportation of the nanoparticles. Using a mouse animal model and retro-orbital sinus injection, we traced in vivo biodistribution to determine the biosafety of the Au-Col-SDF1α nanoparticles. In summary, our results indicate that Au-Col is a promising drug delivery system; it can be used to carry SDF1α to improve MSC therapeutic efficiency. |
| first_indexed | 2024-03-11T01:12:44Z |
| format | Article |
| id | doaj.art-868e6c685f6d453194db3233be567c46 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T01:12:44Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-868e6c685f6d453194db3233be567c462023-11-18T18:46:40ZengMDPI AGCells2073-44092023-07-011214189310.3390/cells12141893Differentiation Induction of Mesenchymal Stem Cells by a Au Delivery PlatformMeng-Yin Yang0Cheng-Di Chiu1Yi-Chun Ke2Yi-Chin Yang3Kai-Bo Chang4Chien-Min Chen5Hsu-Tung Lee6Chien-Lun Tang7Bai-Shuan Liu8Huey-Shan Hung9Department of Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung 407219, TaiwanDepartment of Neurosurgery, China Medical University Hospital, Taichung 404327, TaiwanGraduate Institute of Biomedical Science, China Medical University, Taichung 404333, TaiwanDepartment of Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung 407219, TaiwanGraduate Institute of Biomedical Science, China Medical University, Taichung 404333, TaiwanDivision of Neurosurgery, Department of Surgery, Changhua Christian Hospital, Changhua 50006, TaiwanDepartment of Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung 407219, TaiwanDepartment of Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung 407219, TaiwanDepartment of Medical Imaging and Radiological Sciences, Central Taiwan University of Science and Technology, Taichung 406053, TaiwanGraduate Institute of Biomedical Science, China Medical University, Taichung 404333, TaiwanAu decorated with type I collagen (Col) was used as a core material to cross-link with stromal cell-derived factor 1α (SDF1α) in order to investigate biological performance. The Au-based nanoparticles were subjected to physicochemical determination using scanning electron microscopy (SEM), dynamic light scattering (DLS) and ultraviolet–visible (UV-Vis) and Fourier-transform infrared spectroscopy (FTIR). Mesenchymal stem cells (MSCs) were used to evaluate the biocompatibility of this nanoparticle using the MTT assay and measuring reactive oxygen species (ROS) production. Also, the biological effects of the SDF-1α-conjugated nanoparticles (Au-Col-SDF1α) were assessed and the mechanisms were explored. Furthermore, we investigated the cell differentiation-inducing potential of these conjugated nanoparticles on MSCs toward endothelial cells, neurons, osteoblasts and adipocytes. We then ultimately explored the process of cell entry and transportation of the nanoparticles. Using a mouse animal model and retro-orbital sinus injection, we traced in vivo biodistribution to determine the biosafety of the Au-Col-SDF1α nanoparticles. In summary, our results indicate that Au-Col is a promising drug delivery system; it can be used to carry SDF1α to improve MSC therapeutic efficiency.https://www.mdpi.com/2073-4409/12/14/1893Austromal cell-derived factor 1αmesenchymal stem celldifferentiationdrug delivery |
| spellingShingle | Meng-Yin Yang Cheng-Di Chiu Yi-Chun Ke Yi-Chin Yang Kai-Bo Chang Chien-Min Chen Hsu-Tung Lee Chien-Lun Tang Bai-Shuan Liu Huey-Shan Hung Differentiation Induction of Mesenchymal Stem Cells by a Au Delivery Platform Cells Au stromal cell-derived factor 1α mesenchymal stem cell differentiation drug delivery |
| title | Differentiation Induction of Mesenchymal Stem Cells by a Au Delivery Platform |
| title_full | Differentiation Induction of Mesenchymal Stem Cells by a Au Delivery Platform |
| title_fullStr | Differentiation Induction of Mesenchymal Stem Cells by a Au Delivery Platform |
| title_full_unstemmed | Differentiation Induction of Mesenchymal Stem Cells by a Au Delivery Platform |
| title_short | Differentiation Induction of Mesenchymal Stem Cells by a Au Delivery Platform |
| title_sort | differentiation induction of mesenchymal stem cells by a au delivery platform |
| topic | Au stromal cell-derived factor 1α mesenchymal stem cell differentiation drug delivery |
| url | https://www.mdpi.com/2073-4409/12/14/1893 |
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