Profil Kromatografi Senyawa Aktif Antioksidan dan Antibakteri Fraksi N-Heksana Daun Libo (Ficus variegata Blume)
Research about chromatographic profile of antioxidant and antibacterial compounds in n-hexane fraction of Libo leaves (Ficus variegata Blume.) has been done in order to know the form of chromatographic profile of antioxidant and antibacterial compounds in n-hexane fraction of Libo leaves. Extractio...
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| Format: | Article |
| Language: | English |
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Mulawarman University
2015-12-01
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| Series: | Journal of Tropical Pharmacy and Chemistry |
| Subjects: | |
| Online Access: | https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/98 |
| _version_ | 1797637396152451072 |
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| author | Rolan Rusli Myra Puspha Hardina Fairul Muflihah Agung Rahmadani |
| author_facet | Rolan Rusli Myra Puspha Hardina Fairul Muflihah Agung Rahmadani |
| author_sort | Rolan Rusli |
| collection | DOAJ |
| description |
Research about chromatographic profile of antioxidant and antibacterial compounds in n-hexane fraction of Libo leaves (Ficus variegata Blume.) has been done in order to know the form of chromatographic profile of antioxidant and antibacterial compounds in n-hexane fraction of Libo leaves. Extraction was done by maceration using methanol. Isolation was done by 2 methods, vacuum liquid chromatography (VLC) then followed by conventional column chromatography. Eluent used are n-hexane-ethyl acetate and methanol-chloroform. The results based on conventional column chromatography are as many as 10 fractions. The fractions are being conducted then by a qualitative testing of the antioxidant activity using DPPH method and antibacterial activity test using bioautography TLC method. The results formed as a chromatographic profile of fraction that contain antioxidant and antibacterial activity, which are as many as 5 fractions (NHB1, NHB3, NHB4, NHC1, NHC3) active as an antioxidant and 4 fractions (NHB1, NHC1, NHC2, NHC3) which are active as an antibacterial. The secondary metabolite contents of the fractions are alkaloid, flavonoid and steroid/terpenoid.
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| first_indexed | 2024-03-11T12:48:44Z |
| format | Article |
| id | doaj.art-868fc5c373634434ae7541a9da39f3db |
| institution | Directory Open Access Journal |
| issn | 2087-7099 2407-6090 |
| language | English |
| last_indexed | 2024-03-11T12:48:44Z |
| publishDate | 2015-12-01 |
| publisher | Mulawarman University |
| record_format | Article |
| series | Journal of Tropical Pharmacy and Chemistry |
| spelling | doaj.art-868fc5c373634434ae7541a9da39f3db2023-11-04T06:24:46ZengMulawarman UniversityJournal of Tropical Pharmacy and Chemistry2087-70992407-60902015-12-013210.25026/jtpc.v3i2.9898Profil Kromatografi Senyawa Aktif Antioksidan dan Antibakteri Fraksi N-Heksana Daun Libo (Ficus variegata Blume)Rolan Rusli0Myra Puspha Hardina1Fairul Muflihah2Agung Rahmadani3Laboratorium Penelitian dan Pengembangan Kefarmasian "Farmaka Tropis", Fakultas Farmasi, Universitas Mulawarman, Samarinda, IndonesiaLaboratorium Penelitian dan Pengembangan Kefarmasian "Farmaka Tropis", Fakultas Farmasi, Universitas Mulawarman, Samarinda, IndonesiaLaboratorium Penelitian dan Pengembangan Kefarmasian "Farmaka Tropis", Fakultas Farmasi, Universitas Mulawarman, Samarinda, IndonesiaLaboratorium Penelitian dan Pengembangan Kefarmasian "Farmaka Tropis", Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia Research about chromatographic profile of antioxidant and antibacterial compounds in n-hexane fraction of Libo leaves (Ficus variegata Blume.) has been done in order to know the form of chromatographic profile of antioxidant and antibacterial compounds in n-hexane fraction of Libo leaves. Extraction was done by maceration using methanol. Isolation was done by 2 methods, vacuum liquid chromatography (VLC) then followed by conventional column chromatography. Eluent used are n-hexane-ethyl acetate and methanol-chloroform. The results based on conventional column chromatography are as many as 10 fractions. The fractions are being conducted then by a qualitative testing of the antioxidant activity using DPPH method and antibacterial activity test using bioautography TLC method. The results formed as a chromatographic profile of fraction that contain antioxidant and antibacterial activity, which are as many as 5 fractions (NHB1, NHB3, NHB4, NHC1, NHC3) active as an antioxidant and 4 fractions (NHB1, NHC1, NHC2, NHC3) which are active as an antibacterial. The secondary metabolite contents of the fractions are alkaloid, flavonoid and steroid/terpenoid. https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/98n-hexane fraction of Libo leaves (Ficus variegata Blume.)chromatographyantioxidantantibacterial |
| spellingShingle | Rolan Rusli Myra Puspha Hardina Fairul Muflihah Agung Rahmadani Profil Kromatografi Senyawa Aktif Antioksidan dan Antibakteri Fraksi N-Heksana Daun Libo (Ficus variegata Blume) Journal of Tropical Pharmacy and Chemistry n-hexane fraction of Libo leaves (Ficus variegata Blume.) chromatography antioxidant antibacterial |
| title | Profil Kromatografi Senyawa Aktif Antioksidan dan Antibakteri Fraksi N-Heksana Daun Libo (Ficus variegata Blume) |
| title_full | Profil Kromatografi Senyawa Aktif Antioksidan dan Antibakteri Fraksi N-Heksana Daun Libo (Ficus variegata Blume) |
| title_fullStr | Profil Kromatografi Senyawa Aktif Antioksidan dan Antibakteri Fraksi N-Heksana Daun Libo (Ficus variegata Blume) |
| title_full_unstemmed | Profil Kromatografi Senyawa Aktif Antioksidan dan Antibakteri Fraksi N-Heksana Daun Libo (Ficus variegata Blume) |
| title_short | Profil Kromatografi Senyawa Aktif Antioksidan dan Antibakteri Fraksi N-Heksana Daun Libo (Ficus variegata Blume) |
| title_sort | profil kromatografi senyawa aktif antioksidan dan antibakteri fraksi n heksana daun libo ficus variegata blume |
| topic | n-hexane fraction of Libo leaves (Ficus variegata Blume.) chromatography antioxidant antibacterial |
| url | https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/98 |
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