Enhancing optoacoustic mesoscopy through calibration-based iterative reconstruction
Optoacoustic mesoscopy combines rich optical absorption contrast with high spatial resolution at tissue depths beyond reach for microscopic techniques employing focused light excitation. The mesoscopic imaging performance is commonly hindered by the use of inaccurate delay-and-sum reconstruction app...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-12-01
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| Series: | Photoacoustics |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213597922000702 |
| _version_ | 1811201685106720768 |
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| author | Urs A.T. Hofmann Weiye Li Xosé Luís Deán-Ben Pavel Subochev Héctor Estrada Daniel Razansky |
| author_facet | Urs A.T. Hofmann Weiye Li Xosé Luís Deán-Ben Pavel Subochev Héctor Estrada Daniel Razansky |
| author_sort | Urs A.T. Hofmann |
| collection | DOAJ |
| description | Optoacoustic mesoscopy combines rich optical absorption contrast with high spatial resolution at tissue depths beyond reach for microscopic techniques employing focused light excitation. The mesoscopic imaging performance is commonly hindered by the use of inaccurate delay-and-sum reconstruction approaches and idealized modeling assumptions. In principle, image reconstruction performance could be enhanced by simulating the optoacoustic signal generation, propagation, and detection path. However, for most realistic experimental scenarios, the underlying total impulse response (TIR) cannot be accurately modelled. Here we propose to capture the TIR by scanning of a sub-resolution sized absorber. Significant improvement of spatial resolution and depth uniformity is demonstrated over 3 mm range, outperforming delay-and-sum and model-based reconstruction implementations. Reconstruction performance is validated by imaging subcutaneous murine vasculature and human skin in vivo. The proposed experimental calibration and reconstruction paradigm facilitates quantitative inversions while averting complex physics-based simulations. It can readily be applied to other imaging modalities employing TIR-based reconstructions. |
| first_indexed | 2024-04-12T02:25:44Z |
| format | Article |
| id | doaj.art-868fe809d078419aa1aa99d076e4cea7 |
| institution | Directory Open Access Journal |
| issn | 2213-5979 |
| language | English |
| last_indexed | 2024-04-12T02:25:44Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photoacoustics |
| spelling | doaj.art-868fe809d078419aa1aa99d076e4cea72022-12-22T03:52:00ZengElsevierPhotoacoustics2213-59792022-12-0128100405Enhancing optoacoustic mesoscopy through calibration-based iterative reconstructionUrs A.T. Hofmann0Weiye Li1Xosé Luís Deán-Ben2Pavel Subochev3Héctor Estrada4Daniel Razansky5Institute for Biomedical Engineering and Institute of Pharmacology and Toxicology, Faculty of Medicine, University of Zurich, Switzerland; Institute for Biomedical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, SwitzerlandInstitute for Biomedical Engineering and Institute of Pharmacology and Toxicology, Faculty of Medicine, University of Zurich, Switzerland; Institute for Biomedical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, SwitzerlandInstitute for Biomedical Engineering and Institute of Pharmacology and Toxicology, Faculty of Medicine, University of Zurich, Switzerland; Institute for Biomedical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, SwitzerlandInstitute of Applied Physics, Russian Academy of Sciences, Nizhny Novgorod, RussiaInstitute for Biomedical Engineering and Institute of Pharmacology and Toxicology, Faculty of Medicine, University of Zurich, Switzerland; Institute for Biomedical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, SwitzerlandInstitute for Biomedical Engineering and Institute of Pharmacology and Toxicology, Faculty of Medicine, University of Zurich, Switzerland; Institute for Biomedical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, Switzerland; Correspondence to: Institute for Biomedical Engineering, University of Zurich and ETH Zurich, Switzerland.Optoacoustic mesoscopy combines rich optical absorption contrast with high spatial resolution at tissue depths beyond reach for microscopic techniques employing focused light excitation. The mesoscopic imaging performance is commonly hindered by the use of inaccurate delay-and-sum reconstruction approaches and idealized modeling assumptions. In principle, image reconstruction performance could be enhanced by simulating the optoacoustic signal generation, propagation, and detection path. However, for most realistic experimental scenarios, the underlying total impulse response (TIR) cannot be accurately modelled. Here we propose to capture the TIR by scanning of a sub-resolution sized absorber. Significant improvement of spatial resolution and depth uniformity is demonstrated over 3 mm range, outperforming delay-and-sum and model-based reconstruction implementations. Reconstruction performance is validated by imaging subcutaneous murine vasculature and human skin in vivo. The proposed experimental calibration and reconstruction paradigm facilitates quantitative inversions while averting complex physics-based simulations. It can readily be applied to other imaging modalities employing TIR-based reconstructions.http://www.sciencedirect.com/science/article/pii/S2213597922000702Optoacoustic mesoscopyPhotoacoustic imagingQuantitative reconstructionIterative inversionImpulse responseBiomedical imaging |
| spellingShingle | Urs A.T. Hofmann Weiye Li Xosé Luís Deán-Ben Pavel Subochev Héctor Estrada Daniel Razansky Enhancing optoacoustic mesoscopy through calibration-based iterative reconstruction Photoacoustics Optoacoustic mesoscopy Photoacoustic imaging Quantitative reconstruction Iterative inversion Impulse response Biomedical imaging |
| title | Enhancing optoacoustic mesoscopy through calibration-based iterative reconstruction |
| title_full | Enhancing optoacoustic mesoscopy through calibration-based iterative reconstruction |
| title_fullStr | Enhancing optoacoustic mesoscopy through calibration-based iterative reconstruction |
| title_full_unstemmed | Enhancing optoacoustic mesoscopy through calibration-based iterative reconstruction |
| title_short | Enhancing optoacoustic mesoscopy through calibration-based iterative reconstruction |
| title_sort | enhancing optoacoustic mesoscopy through calibration based iterative reconstruction |
| topic | Optoacoustic mesoscopy Photoacoustic imaging Quantitative reconstruction Iterative inversion Impulse response Biomedical imaging |
| url | http://www.sciencedirect.com/science/article/pii/S2213597922000702 |
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