Vigilancia activa de infecciones asociadas a la atención en salud en unidades de cuidado intensivo: perfil de resistencia y molecular en un país en desarrollo
Objective: This study aimed to characterize epidemiological and molecular profile of Healthcare-associated infections [HAI] in 21 intensive care units (ICU) in a city in Colombia.Methods:Descriptive study of prevalence. Adult patients were screened in 21 ICUs for HAIs: VAP, CLABSI; CAUTI and/or SSI....
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| Format: | Article |
| Language: | English |
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Asociación Colombiana de Infectología
2022-08-01
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| Series: | Infectio |
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| _version_ | 1797997349520277504 |
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| author | Margarita Maria Ochoa-Diaz |
| author_facet | Margarita Maria Ochoa-Diaz |
| author_sort | Margarita Maria Ochoa-Diaz |
| collection | DOAJ |
| description | Objective: This study aimed to characterize epidemiological and molecular profile of Healthcare-associated infections [HAI] in 21 intensive care units (ICU) in a city in Colombia.Methods:Descriptive study of prevalence. Adult patients were screened in 21 ICUs for HAIs: VAP, CLABSI; CAUTI and/or SSI. Microbiological and genotypic iden-tification was performed.Results: Prevalence of HAIs was 41.4% (CI 36.9-45.9).VAP 15.8% (CI 12.7-19.4); CLABSI, 13.5% (CI 10.6-16.9); CAUTI, 7.7% (CI 5.5-10.5); and SSI, 4.4% (CI 2.7-6.6). Gram-negative bacteria (71.7%) predominated (P. aeruginosa (19.1%), K. pneumoniae (13.4%) and E. coli (13%)). Pseudomonas spp. 20-30% were resistant to car-bapenems and ≥ 10% to aztreonam, 3rd- and 4th-generation cephalosporins, and β-lactamase inhibitors. In VAP and CLABSI, 30% of Staphylococcus aureus were resistant to oxacillin. In CAUTI, Staphylococcus epidermidis exhibited 100% resistance. In P. aeruginosa resistance gene were blaTEM, blaSHV, and blaCTX-M (15-32%), KPC (5.7%), and oxacillinases blaOXA-48 (1.8%) and blaOXA-1-40-30 (20-50%). In E. coli, genes qnrB, qnrS and qnrD were identified. In CLABSI, ermC-type (16.7%), aph[2’]’If (7.7%) and ant[4’]-Ia (7.7%) were identified in Staphylococcus aureus. Conclusions: VAP and CLABSI predominate in ICUs evaluated in Colombia due to resistant gram-negative bacteria by ESBL-type resistance genes plasmids, efflux pumps hindering the therapeutic approach. |
| first_indexed | 2024-04-11T10:31:22Z |
| format | Article |
| id | doaj.art-86924a80812345e6ba849d6e442de02f |
| institution | Directory Open Access Journal |
| issn | 0123-9392 |
| language | English |
| last_indexed | 2024-04-11T10:31:22Z |
| publishDate | 2022-08-01 |
| publisher | Asociación Colombiana de Infectología |
| record_format | Article |
| series | Infectio |
| spelling | doaj.art-86924a80812345e6ba849d6e442de02f2022-12-22T04:29:25ZengAsociación Colombiana de InfectologíaInfectio0123-93922022-08-01263230237https://doi.org/10.22354/24223794.1077Vigilancia activa de infecciones asociadas a la atención en salud en unidades de cuidado intensivo: perfil de resistencia y molecular en un país en desarrolloMargarita Maria Ochoa-Diaz0230ARTÍCULO ORIGINALActive surveillance of adult healthcare-associated infections in intensive care units: resistance and molecular profile in an upper middle-income countryMargarita Maria Ochoa-Diaz1,2, Eduardo Santero-Santurino3, Amando Flores-Diaz3, Eva Camacho-Fernández3, María Paulina Osorio-Cortina4, Doris Gómez-Camargo1,21 Doctorate in Tropical Medicine, Universidad de Cartagena, Cartagena, Co-lombia. 2 Grupo UNIMOL, Universidad de Cartagena, Cartagena, ColombiaObjective: This study aimed to characterize epidemiological and molecular profile of Healthcare-associated infections [HAI] in 21 intensive care units (ICU) in a city in Colombia.Methods:Descriptive study of prevalence. Adult patients were screened in 21 ICUs for HAIs: VAP, CLABSI; CAUTI and/or SSI. Microbiological and genotypic iden-tification was performed.Results: Prevalence of HAIs was 41.4% (CI 36.9-45.9).VAP 15.8% (CI 12.7-19.4); CLABSI, 13.5% (CI 10.6-16.9); CAUTI, 7.7% (CI 5.5-10.5); and SSI, 4.4% (CI 2.7-6.6). Gram-negative bacteria (71.7%) predominated (P. aeruginosa (19.1%), K. pneumoniae (13.4%) and E. coli (13%)). Pseudomonas spp. 20-30% were resistant to car-bapenems and ≥ 10% to aztreonam, 3rd- and 4th-generation cephalosporins, and β-lactamase inhibitors. In VAP and CLABSI, 30% of Staphylococcus aureus were resistant to oxacillin. In CAUTI, Staphylococcus epidermidis exhibited 100% resistance. In P. aeruginosa resistance gene were blaTEM, blaSHV, and blaCTX-M (15-32%), KPC (5.7%), and oxacillinases blaOXA-48 (1.8%) and blaOXA-1-40-30 (20-50%). In E. coli, genes qnrB, qnrS and qnrD were identified. In CLABSI, ermC-type (16.7%), aph[2’]’If (7.7%) and ant[4’]-Ia (7.7%) were identified in Staphylococcus aureus. Conclusions: VAP and CLABSI predominate in ICUs evaluated in Colombia due to resistant gram-negative bacteria by ESBL-type resistance genes plasmids, efflux pumps hindering the therapeutic approach.molecular epidemiology; infection control; genotypingcolombia |
| spellingShingle | Margarita Maria Ochoa-Diaz Vigilancia activa de infecciones asociadas a la atención en salud en unidades de cuidado intensivo: perfil de resistencia y molecular en un país en desarrollo Infectio molecular epidemiology; infection control; genotyping colombia |
| title | Vigilancia activa de infecciones asociadas a la atención en salud en unidades de cuidado intensivo: perfil de resistencia y molecular en un país en desarrollo |
| title_full | Vigilancia activa de infecciones asociadas a la atención en salud en unidades de cuidado intensivo: perfil de resistencia y molecular en un país en desarrollo |
| title_fullStr | Vigilancia activa de infecciones asociadas a la atención en salud en unidades de cuidado intensivo: perfil de resistencia y molecular en un país en desarrollo |
| title_full_unstemmed | Vigilancia activa de infecciones asociadas a la atención en salud en unidades de cuidado intensivo: perfil de resistencia y molecular en un país en desarrollo |
| title_short | Vigilancia activa de infecciones asociadas a la atención en salud en unidades de cuidado intensivo: perfil de resistencia y molecular en un país en desarrollo |
| title_sort | vigilancia activa de infecciones asociadas a la atencion en salud en unidades de cuidado intensivo perfil de resistencia y molecular en un pais en desarrollo |
| topic | molecular epidemiology; infection control; genotyping colombia |
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