The use of untargeted and widely targeted metabolomics to distinguish between asphyxia and sudden cardiac death as the cause of death in rats: A preliminary study
It is important to determine the cause of death in the case of asphyxia. However, it is difficult to conclude death by asphyxia, especially when the deceased has underlying heart disease, because there are often no specific and representative corpse signs for both asphyxia and sudden cardiac death (...
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| Format: | Article |
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Elsevier
2022-12-01
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| Series: | Arabian Journal of Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535222006384 |
| _version_ | 1811179789721010176 |
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| author | Kai Zhang Ya Tuo Ruina Liu Hui Yan Ping Xiang Zhenyuan Wang Ping Huang |
| author_facet | Kai Zhang Ya Tuo Ruina Liu Hui Yan Ping Xiang Zhenyuan Wang Ping Huang |
| author_sort | Kai Zhang |
| collection | DOAJ |
| description | It is important to determine the cause of death in the case of asphyxia. However, it is difficult to conclude death by asphyxia, especially when the deceased has underlying heart disease, because there are often no specific and representative corpse signs for both asphyxia and sudden cardiac death (SCD). The aim of the present work was to investigate the potential of metabolomics to discriminate asphyxia from SCD as the cause of death. A total of thirty male Sprague–Dawley rats were used to construct models of asphyxia, SCD (interfering cause of death), and cervical dislocation (control). Untargeted and widely targeted metabolomics approaches were used to obtain rat pulmonary metabolic profiles in this study. First, the metabolic alterations resulting from asphyxia were explored. There were significant changes found in carbohydrate metabolism, the endocrine system, and the sensory system. Second, we screened potential biomarkers and built classification models to determine the cause of death. Moreover, some biomarkers remained differentiated at 24 h and 48 h postmortem, so the cause of death could still be determined after death. This study showed the application potential of metabolomics to investigate the metabolic changes occurring in the process of death, as well as to determine the cause of death on the basis of metabolic differences even after death. |
| first_indexed | 2024-04-11T06:40:35Z |
| format | Article |
| id | doaj.art-8693550b0ae642058f84de08f2a59871 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-04-11T06:40:35Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-8693550b0ae642058f84de08f2a598712022-12-22T04:39:34ZengElsevierArabian Journal of Chemistry1878-53522022-12-011512104322The use of untargeted and widely targeted metabolomics to distinguish between asphyxia and sudden cardiac death as the cause of death in rats: A preliminary studyKai Zhang0Ya Tuo1Ruina Liu2Hui Yan3Ping Xiang4Zhenyuan Wang5Ping Huang6Department of Forensic Pathology, College of Forensic Medicine, Xi'an Jiaotong University, Xi’an 710061, PR ChinaDepartment of Biochemistry and Physiology, Shanghai University of Medicine and Health Sciences, Shanghai 201318, PR ChinaDepartment of Forensic Pathology, College of Forensic Medicine, Xi'an Jiaotong University, Xi’an 710061, PR ChinaShanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, PR ChinaShanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, PR ChinaDepartment of Forensic Pathology, College of Forensic Medicine, Xi'an Jiaotong University, Xi’an 710061, PR China; Corresponding authors.Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, PR China; Corresponding authors.It is important to determine the cause of death in the case of asphyxia. However, it is difficult to conclude death by asphyxia, especially when the deceased has underlying heart disease, because there are often no specific and representative corpse signs for both asphyxia and sudden cardiac death (SCD). The aim of the present work was to investigate the potential of metabolomics to discriminate asphyxia from SCD as the cause of death. A total of thirty male Sprague–Dawley rats were used to construct models of asphyxia, SCD (interfering cause of death), and cervical dislocation (control). Untargeted and widely targeted metabolomics approaches were used to obtain rat pulmonary metabolic profiles in this study. First, the metabolic alterations resulting from asphyxia were explored. There were significant changes found in carbohydrate metabolism, the endocrine system, and the sensory system. Second, we screened potential biomarkers and built classification models to determine the cause of death. Moreover, some biomarkers remained differentiated at 24 h and 48 h postmortem, so the cause of death could still be determined after death. This study showed the application potential of metabolomics to investigate the metabolic changes occurring in the process of death, as well as to determine the cause of death on the basis of metabolic differences even after death.http://www.sciencedirect.com/science/article/pii/S1878535222006384Widely targeted metabolomicsCause of deathAsphyxiaSudden cardiac deathAfter death |
| spellingShingle | Kai Zhang Ya Tuo Ruina Liu Hui Yan Ping Xiang Zhenyuan Wang Ping Huang The use of untargeted and widely targeted metabolomics to distinguish between asphyxia and sudden cardiac death as the cause of death in rats: A preliminary study Arabian Journal of Chemistry Widely targeted metabolomics Cause of death Asphyxia Sudden cardiac death After death |
| title | The use of untargeted and widely targeted metabolomics to distinguish between asphyxia and sudden cardiac death as the cause of death in rats: A preliminary study |
| title_full | The use of untargeted and widely targeted metabolomics to distinguish between asphyxia and sudden cardiac death as the cause of death in rats: A preliminary study |
| title_fullStr | The use of untargeted and widely targeted metabolomics to distinguish between asphyxia and sudden cardiac death as the cause of death in rats: A preliminary study |
| title_full_unstemmed | The use of untargeted and widely targeted metabolomics to distinguish between asphyxia and sudden cardiac death as the cause of death in rats: A preliminary study |
| title_short | The use of untargeted and widely targeted metabolomics to distinguish between asphyxia and sudden cardiac death as the cause of death in rats: A preliminary study |
| title_sort | use of untargeted and widely targeted metabolomics to distinguish between asphyxia and sudden cardiac death as the cause of death in rats a preliminary study |
| topic | Widely targeted metabolomics Cause of death Asphyxia Sudden cardiac death After death |
| url | http://www.sciencedirect.com/science/article/pii/S1878535222006384 |
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