Antibody Evaluation and Mutations of Antigenic Epitopes in the Spike Protein of the Porcine Epidemic Diarrhea Virus from Pig Farms with Repeated Intentional Exposure (Feedback)
The feedback strategy, or controlled exposure of pig herd to the porcine epidemic diarrhea virus (PEDV), significantly decreased losses during a severe outbreak in late 2013 in Taiwan. However, some pig farms still suffered from recurrent outbreaks. To evaluate the association between antibody titer...
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MDPI AG
2022-03-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/3/551 |
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| author | Thu Hien Nguyen Thi Chi-Chih Chen Wen-Bin Chung Hso-Chi Chaung Yen-Li Huang Li-Ting Cheng Guan-Ming Ke |
| author_facet | Thu Hien Nguyen Thi Chi-Chih Chen Wen-Bin Chung Hso-Chi Chaung Yen-Li Huang Li-Ting Cheng Guan-Ming Ke |
| author_sort | Thu Hien Nguyen Thi |
| collection | DOAJ |
| description | The feedback strategy, or controlled exposure of pig herd to the porcine epidemic diarrhea virus (PEDV), significantly decreased losses during a severe outbreak in late 2013 in Taiwan. However, some pig farms still suffered from recurrent outbreaks. To evaluate the association between antibody titers and clinical manifestations, sera and colostra were analyzed from one pig farm that employed the feedback strategy. Furthermore, spike (S) gene full sequences from six positive samples of two farms with and without using feedback were compared to investigate the evolution of PEDV variants circulating in pig herds. The results in this study showed that high PEDV antibody titers do not correlate with the high rate of protection from PEDV infection. In addition, repeated feedback generated the emergence of PEDV variants with unique substitutions of N537S and Y561H in the COE domain and S769F in the SS6 epitopes. These mutations indicated the pathogenetic evolution of PEDV strains existing in the cycle of the feedback method. A very strict biosecurity practice to block the routes of pathogen transfer should be followed to achieve successful control of PEDV infections in pig herds. |
| first_indexed | 2024-03-09T12:16:55Z |
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| id | doaj.art-8694824cf1dc43d78763ea92a3e4c3a7 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T12:16:55Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-8694824cf1dc43d78763ea92a3e4c3a72023-11-30T22:46:09ZengMDPI AGViruses1999-49152022-03-0114355110.3390/v14030551Antibody Evaluation and Mutations of Antigenic Epitopes in the Spike Protein of the Porcine Epidemic Diarrhea Virus from Pig Farms with Repeated Intentional Exposure (Feedback)Thu Hien Nguyen Thi0Chi-Chih Chen1Wen-Bin Chung2Hso-Chi Chaung3Yen-Li Huang4Li-Ting Cheng5Guan-Ming Ke6International Degree Program of Animal Vaccine Technology, International College, National Pingtung University of Science and Technology, No.1, Shuefu Road, Neipu, Pingtung 91201, TaiwanResearch Centre for Animal Biologics, National Pingtung University of Science and Technology, No. 1 Shuefu Road, Neipu, Pingtung 91201, TaiwanResearch Centre for Animal Biologics, National Pingtung University of Science and Technology, No. 1 Shuefu Road, Neipu, Pingtung 91201, TaiwanResearch Centre for Animal Biologics, National Pingtung University of Science and Technology, No. 1 Shuefu Road, Neipu, Pingtung 91201, TaiwanResearch Centre for Animal Biologics, National Pingtung University of Science and Technology, No. 1 Shuefu Road, Neipu, Pingtung 91201, TaiwanGraduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and Technology, No. 1 Shuefu Road, Neipu, Pingtung 91201, TaiwanResearch Centre for Animal Biologics, National Pingtung University of Science and Technology, No. 1 Shuefu Road, Neipu, Pingtung 91201, TaiwanThe feedback strategy, or controlled exposure of pig herd to the porcine epidemic diarrhea virus (PEDV), significantly decreased losses during a severe outbreak in late 2013 in Taiwan. However, some pig farms still suffered from recurrent outbreaks. To evaluate the association between antibody titers and clinical manifestations, sera and colostra were analyzed from one pig farm that employed the feedback strategy. Furthermore, spike (S) gene full sequences from six positive samples of two farms with and without using feedback were compared to investigate the evolution of PEDV variants circulating in pig herds. The results in this study showed that high PEDV antibody titers do not correlate with the high rate of protection from PEDV infection. In addition, repeated feedback generated the emergence of PEDV variants with unique substitutions of N537S and Y561H in the COE domain and S769F in the SS6 epitopes. These mutations indicated the pathogenetic evolution of PEDV strains existing in the cycle of the feedback method. A very strict biosecurity practice to block the routes of pathogen transfer should be followed to achieve successful control of PEDV infections in pig herds.https://www.mdpi.com/1999-4915/14/3/551porcine epidemic diarrheaphylogenetic analysislactogenic immunityspike geneantigen epitopesfeedback |
| spellingShingle | Thu Hien Nguyen Thi Chi-Chih Chen Wen-Bin Chung Hso-Chi Chaung Yen-Li Huang Li-Ting Cheng Guan-Ming Ke Antibody Evaluation and Mutations of Antigenic Epitopes in the Spike Protein of the Porcine Epidemic Diarrhea Virus from Pig Farms with Repeated Intentional Exposure (Feedback) Viruses porcine epidemic diarrhea phylogenetic analysis lactogenic immunity spike gene antigen epitopes feedback |
| title | Antibody Evaluation and Mutations of Antigenic Epitopes in the Spike Protein of the Porcine Epidemic Diarrhea Virus from Pig Farms with Repeated Intentional Exposure (Feedback) |
| title_full | Antibody Evaluation and Mutations of Antigenic Epitopes in the Spike Protein of the Porcine Epidemic Diarrhea Virus from Pig Farms with Repeated Intentional Exposure (Feedback) |
| title_fullStr | Antibody Evaluation and Mutations of Antigenic Epitopes in the Spike Protein of the Porcine Epidemic Diarrhea Virus from Pig Farms with Repeated Intentional Exposure (Feedback) |
| title_full_unstemmed | Antibody Evaluation and Mutations of Antigenic Epitopes in the Spike Protein of the Porcine Epidemic Diarrhea Virus from Pig Farms with Repeated Intentional Exposure (Feedback) |
| title_short | Antibody Evaluation and Mutations of Antigenic Epitopes in the Spike Protein of the Porcine Epidemic Diarrhea Virus from Pig Farms with Repeated Intentional Exposure (Feedback) |
| title_sort | antibody evaluation and mutations of antigenic epitopes in the spike protein of the porcine epidemic diarrhea virus from pig farms with repeated intentional exposure feedback |
| topic | porcine epidemic diarrhea phylogenetic analysis lactogenic immunity spike gene antigen epitopes feedback |
| url | https://www.mdpi.com/1999-4915/14/3/551 |
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