A walk on the wild side: gut bacteria fed to mass-reared larvae of Queensland fruit fly [Bactrocera tryoni (Froggatt)] influence development
Abstract Background The Queensland fruit fly, Bactrocera tryoni (Froggatt) (Diptera, Tephritidae) is the most significant insect pest of Australian horticulture. Bactrocera tryoni is controlled using a range of tools including the Sterile Insect Technique (SIT). Mass-rearing and irradiation of pupae...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-12-01
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| Series: | BMC Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12896-019-0579-6 |
| _version_ | 1819058155572690944 |
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| author | Lucas Alexander Shuttleworth Mohammed Abul Monjur Khan Terrence Osborne Damian Collins Mukesh Srivastava Olivia Louise Reynolds |
| author_facet | Lucas Alexander Shuttleworth Mohammed Abul Monjur Khan Terrence Osborne Damian Collins Mukesh Srivastava Olivia Louise Reynolds |
| author_sort | Lucas Alexander Shuttleworth |
| collection | DOAJ |
| description | Abstract Background The Queensland fruit fly, Bactrocera tryoni (Froggatt) (Diptera, Tephritidae) is the most significant insect pest of Australian horticulture. Bactrocera tryoni is controlled using a range of tools including the Sterile Insect Technique (SIT). Mass-rearing and irradiation of pupae in SIT can reduce the fitness and quality of the released sterile insects. Studies have also showed reduced microbial gut diversity in domesticated versus wild tephritids. Results Transmission electron microscopy confirmed the presence of the bacterial isolates in the mid-gut of mass-reared larvae, and plate counts from individual larval guts showed increased numbers of bacteria in supplemented larvae. Several developmental and fitness parameters were tested including larval development time (egg-hatch to pupation), pupal weight, emergence, flight ability, sex-ratio, and time to adult eclosion (egg-hatch to adult eclosion). Enterobacter sp. and Asaia sp. shortened larval development time, while this was delayed by Lactobacillus sp., Leuconostoc sp. and a blend of all four bacteria. The mean time from egg hatch to adult eclosion was significantly reduced by Leuconostoc sp. and the blend for males and females, indicating that the individual bacterium and consortium affect flies differently depending on the life stage (larval or pupal). There was no impact of bacterial supplemented larvae on pupal weight, emergence, flight ability, or sex ratio. Conclusions Our findings show that bacteria fed to the larval stage of B. tryoni can impart fitness advantages, but the selection of probiotic strains (individual or a consortium) is key, as each have varying effects on the host. Bacteria added to the larval diet particularly Leuconostoc sp. and the blend have the capacity to reduce costs and increase the number of flies produced in mass-rearing facilities by reducing time to adult eclosion by 1.3 and 0.8 mean days for males, and 1.2 and 0.8 mean days for females. |
| first_indexed | 2024-12-21T13:50:42Z |
| format | Article |
| id | doaj.art-869a2731ff3748319ce5bec6f84741db |
| institution | Directory Open Access Journal |
| issn | 1472-6750 |
| language | English |
| last_indexed | 2024-12-21T13:50:42Z |
| publishDate | 2019-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj.art-869a2731ff3748319ce5bec6f84741db2022-12-21T19:01:42ZengBMCBMC Biotechnology1472-67502019-12-0119S211110.1186/s12896-019-0579-6A walk on the wild side: gut bacteria fed to mass-reared larvae of Queensland fruit fly [Bactrocera tryoni (Froggatt)] influence developmentLucas Alexander Shuttleworth0Mohammed Abul Monjur Khan1Terrence Osborne2Damian Collins3Mukesh Srivastava4Olivia Louise Reynolds5Biosecurity and Food Safety, New South Wales Department of Primary Industries, Elizabeth Macarthur Agricultural InstituteBiosecurity and Food Safety, New South Wales Department of Primary Industries, Elizabeth Macarthur Agricultural InstituteBiosecurity and Food Safety, New South Wales Department of Primary Industries, Elizabeth Macarthur Agricultural InstituteBiosecurity and Food Safety, New South Wales Department of Primary Industries, Elizabeth Macarthur Agricultural InstituteBiosecurity and Food Safety, New South Wales Department of Primary Industries, Elizabeth Macarthur Agricultural InstituteBiosecurity and Food Safety, New South Wales Department of Primary Industries, Elizabeth Macarthur Agricultural InstituteAbstract Background The Queensland fruit fly, Bactrocera tryoni (Froggatt) (Diptera, Tephritidae) is the most significant insect pest of Australian horticulture. Bactrocera tryoni is controlled using a range of tools including the Sterile Insect Technique (SIT). Mass-rearing and irradiation of pupae in SIT can reduce the fitness and quality of the released sterile insects. Studies have also showed reduced microbial gut diversity in domesticated versus wild tephritids. Results Transmission electron microscopy confirmed the presence of the bacterial isolates in the mid-gut of mass-reared larvae, and plate counts from individual larval guts showed increased numbers of bacteria in supplemented larvae. Several developmental and fitness parameters were tested including larval development time (egg-hatch to pupation), pupal weight, emergence, flight ability, sex-ratio, and time to adult eclosion (egg-hatch to adult eclosion). Enterobacter sp. and Asaia sp. shortened larval development time, while this was delayed by Lactobacillus sp., Leuconostoc sp. and a blend of all four bacteria. The mean time from egg hatch to adult eclosion was significantly reduced by Leuconostoc sp. and the blend for males and females, indicating that the individual bacterium and consortium affect flies differently depending on the life stage (larval or pupal). There was no impact of bacterial supplemented larvae on pupal weight, emergence, flight ability, or sex ratio. Conclusions Our findings show that bacteria fed to the larval stage of B. tryoni can impart fitness advantages, but the selection of probiotic strains (individual or a consortium) is key, as each have varying effects on the host. Bacteria added to the larval diet particularly Leuconostoc sp. and the blend have the capacity to reduce costs and increase the number of flies produced in mass-rearing facilities by reducing time to adult eclosion by 1.3 and 0.8 mean days for males, and 1.2 and 0.8 mean days for females.https://doi.org/10.1186/s12896-019-0579-6AsaiaEnterobacterLactobacillusLeuconostocSterile insect techniqueArea wide - integrated Pest management |
| spellingShingle | Lucas Alexander Shuttleworth Mohammed Abul Monjur Khan Terrence Osborne Damian Collins Mukesh Srivastava Olivia Louise Reynolds A walk on the wild side: gut bacteria fed to mass-reared larvae of Queensland fruit fly [Bactrocera tryoni (Froggatt)] influence development BMC Biotechnology Asaia Enterobacter Lactobacillus Leuconostoc Sterile insect technique Area wide - integrated Pest management |
| title | A walk on the wild side: gut bacteria fed to mass-reared larvae of Queensland fruit fly [Bactrocera tryoni (Froggatt)] influence development |
| title_full | A walk on the wild side: gut bacteria fed to mass-reared larvae of Queensland fruit fly [Bactrocera tryoni (Froggatt)] influence development |
| title_fullStr | A walk on the wild side: gut bacteria fed to mass-reared larvae of Queensland fruit fly [Bactrocera tryoni (Froggatt)] influence development |
| title_full_unstemmed | A walk on the wild side: gut bacteria fed to mass-reared larvae of Queensland fruit fly [Bactrocera tryoni (Froggatt)] influence development |
| title_short | A walk on the wild side: gut bacteria fed to mass-reared larvae of Queensland fruit fly [Bactrocera tryoni (Froggatt)] influence development |
| title_sort | walk on the wild side gut bacteria fed to mass reared larvae of queensland fruit fly bactrocera tryoni froggatt influence development |
| topic | Asaia Enterobacter Lactobacillus Leuconostoc Sterile insect technique Area wide - integrated Pest management |
| url | https://doi.org/10.1186/s12896-019-0579-6 |
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