Evaluation of copper concentration in subclinical cases of white muscle disease and its relationship with cardiac troponin I.
The present study aims to evaluate the serum level of copper (Cu) in lambs suffering from subclinical forms of white muscle disease (WMD) and its relationship with cardiac troponin I (cTn-I) as a novel biomarker of cardiovascular disorders. Ten milliliters of jugular blood were taken from 200 lambs...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3568093?pdf=render |
| _version_ | 1828402341884198912 |
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| author | Forough Ataollahi Mehrdad Mohri Hesam A Seifi Belinda Pingguan-Murphy Wan Abu Bakar Wan Abas Noor Azuan Abu Osman |
| author_facet | Forough Ataollahi Mehrdad Mohri Hesam A Seifi Belinda Pingguan-Murphy Wan Abu Bakar Wan Abas Noor Azuan Abu Osman |
| author_sort | Forough Ataollahi |
| collection | DOAJ |
| description | The present study aims to evaluate the serum level of copper (Cu) in lambs suffering from subclinical forms of white muscle disease (WMD) and its relationship with cardiac troponin I (cTn-I) as a novel biomarker of cardiovascular disorders. Ten milliliters of jugular blood were taken from 200 lambs less than one year old to measure serum concentrations of Cu, selenium (Se), and cTn-I. The subjects were divided into 2 groups, namely, the deficient group which included 36 lambs, and the control group which included 164 lambs according to the reference serum Se concentration (50 ng/mL). Serum Se levels in the deficient group were lower than 50 ng/mL. By contrast, the control group showed Se levels higher than 50 ng/mL. Differences among the serum Cu and cTn-I levels were determined in both groups. The mean ±SD and median of serum Cu and cTn-I levels in the deficient group were lower and higher than those in the control group, respectively. A significant positive correlation was observed between serum Cu and Se levels, and also serum Cu and Se levels showed a negative correlation with serum cTn-I concentrations. Stepwise linear regression analysis showed that serum Cu levels were correlated positively with serum Se levels (p<0.05). Receiver operating characteristic (ROC) curve analysis indicated that the area under curve (AUC) of Cu was significantly higher than that of cTn-I based on the reference diagonal line. It is important to keep in mind that the value of AUC for the ROC curve is between 0.5 and 1.00, in which the lowest accuracy is related to the reference diagonal line with AUC of 0.5. A cut-off was determined to indicate which Cu level can discriminate between affected and healthy lambs. The cut-off level, sensitivity, and specificity of Cu in this study were 144.5 ng/mL, 74%, and 61%, respectively. |
| first_indexed | 2024-12-10T09:58:50Z |
| format | Article |
| id | doaj.art-869abb94f288437ea7bb8500988a50c7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T09:58:50Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-869abb94f288437ea7bb8500988a50c72022-12-22T01:53:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0182e5616310.1371/journal.pone.0056163Evaluation of copper concentration in subclinical cases of white muscle disease and its relationship with cardiac troponin I.Forough AtaollahiMehrdad MohriHesam A SeifiBelinda Pingguan-MurphyWan Abu Bakar Wan AbasNoor Azuan Abu OsmanThe present study aims to evaluate the serum level of copper (Cu) in lambs suffering from subclinical forms of white muscle disease (WMD) and its relationship with cardiac troponin I (cTn-I) as a novel biomarker of cardiovascular disorders. Ten milliliters of jugular blood were taken from 200 lambs less than one year old to measure serum concentrations of Cu, selenium (Se), and cTn-I. The subjects were divided into 2 groups, namely, the deficient group which included 36 lambs, and the control group which included 164 lambs according to the reference serum Se concentration (50 ng/mL). Serum Se levels in the deficient group were lower than 50 ng/mL. By contrast, the control group showed Se levels higher than 50 ng/mL. Differences among the serum Cu and cTn-I levels were determined in both groups. The mean ±SD and median of serum Cu and cTn-I levels in the deficient group were lower and higher than those in the control group, respectively. A significant positive correlation was observed between serum Cu and Se levels, and also serum Cu and Se levels showed a negative correlation with serum cTn-I concentrations. Stepwise linear regression analysis showed that serum Cu levels were correlated positively with serum Se levels (p<0.05). Receiver operating characteristic (ROC) curve analysis indicated that the area under curve (AUC) of Cu was significantly higher than that of cTn-I based on the reference diagonal line. It is important to keep in mind that the value of AUC for the ROC curve is between 0.5 and 1.00, in which the lowest accuracy is related to the reference diagonal line with AUC of 0.5. A cut-off was determined to indicate which Cu level can discriminate between affected and healthy lambs. The cut-off level, sensitivity, and specificity of Cu in this study were 144.5 ng/mL, 74%, and 61%, respectively.http://europepmc.org/articles/PMC3568093?pdf=render |
| spellingShingle | Forough Ataollahi Mehrdad Mohri Hesam A Seifi Belinda Pingguan-Murphy Wan Abu Bakar Wan Abas Noor Azuan Abu Osman Evaluation of copper concentration in subclinical cases of white muscle disease and its relationship with cardiac troponin I. PLoS ONE |
| title | Evaluation of copper concentration in subclinical cases of white muscle disease and its relationship with cardiac troponin I. |
| title_full | Evaluation of copper concentration in subclinical cases of white muscle disease and its relationship with cardiac troponin I. |
| title_fullStr | Evaluation of copper concentration in subclinical cases of white muscle disease and its relationship with cardiac troponin I. |
| title_full_unstemmed | Evaluation of copper concentration in subclinical cases of white muscle disease and its relationship with cardiac troponin I. |
| title_short | Evaluation of copper concentration in subclinical cases of white muscle disease and its relationship with cardiac troponin I. |
| title_sort | evaluation of copper concentration in subclinical cases of white muscle disease and its relationship with cardiac troponin i |
| url | http://europepmc.org/articles/PMC3568093?pdf=render |
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