Modified preservation and fungalysin description for Batrachochytrium dendrobatidis
Batrachochytrium dendrobatidis is a pathogenic fungus causing chytridiomycosis, a cutaneous affection resulting in reduction on Anura populations around the world, because of fungalysins, the most important virulence factor. The B. dendrobatidis maps (http://www.bd-maps.net/) is the dataset of info...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universidad Politécnica Salesiana
2022-08-01
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| Series: | La Granja: Revista de Ciencias de la Vida |
| Subjects: | |
| Online Access: | https://revistas.ups.edu.ec/index.php/granja/article/view/4905 |
| _version_ | 1818058362418364416 |
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| author | Olga Cristina Lozano Camelo Adriana Carolina Rojas Arias Kelly Johanna Ávila Méndez Silvio Alejandro Lopez-Pazos |
| author_facet | Olga Cristina Lozano Camelo Adriana Carolina Rojas Arias Kelly Johanna Ávila Méndez Silvio Alejandro Lopez-Pazos |
| author_sort | Olga Cristina Lozano Camelo |
| collection | DOAJ |
| description |
Batrachochytrium dendrobatidis is a pathogenic fungus causing chytridiomycosis, a cutaneous affection resulting in reduction on Anura populations around the world, because of fungalysins, the most important virulence factor. The B. dendrobatidis maps (http://www.bd-maps.net/) is the dataset of information about the B. dendrobatidis -related isolates. An alternative available for reconstitution of B. dendrobatidis strains from cryopreserved cells include methods relying on the World Organization for Animal Health (OIE) protocol. Most recently, much of the interest in the B. dendrobatidis research has focused on its DNA sequencing, especially B. dendrobatidis JAM81 and B. dendrobatidis JEL423 genomes. OBJECTIVE. To evaluate a modified form from OIE protocol for B. dendrobatidis strain criopreservation, and in silico analysis of B. dendrobatidis fungalysin. OIE protocol was modified using B. dendrobatidis EV001, focused on cryopreservant concentrations, antibiotics and recovering to -80°C, and microscopic viability evaluation on Tryptone Gelatin hydrolyzed agar, besides, Bioinformatics was used for the determination of biochemical characteristics from a B. dendrobatidis JAM81 fungalysin. Modified OIE protocol was useful by viability, recovering B. dendrobatidis EV001 strain. The characterization of B. dendrobatidis JAM81 fungalysin showed a molecular weight of 85 kDa, isoelectric point of 8.33, and tertiary structure among others. This indicated that the protein is a metalloproteinase, it has a PepSY domain for protease inhibition, and a catalytic domain that destroy protein barriers.
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| first_indexed | 2024-12-10T12:59:25Z |
| format | Article |
| id | doaj.art-869b6b86de5b484ca2beaf01d015fdbf |
| institution | Directory Open Access Journal |
| issn | 1390-3799 1390-8596 |
| language | English |
| last_indexed | 2024-12-10T12:59:25Z |
| publishDate | 2022-08-01 |
| publisher | Universidad Politécnica Salesiana |
| record_format | Article |
| series | La Granja: Revista de Ciencias de la Vida |
| spelling | doaj.art-869b6b86de5b484ca2beaf01d015fdbf2022-12-22T01:47:59ZengUniversidad Politécnica SalesianaLa Granja: Revista de Ciencias de la Vida1390-37991390-85962022-08-01362Modified preservation and fungalysin description for Batrachochytrium dendrobatidisOlga Cristina Lozano Camelo0Adriana Carolina Rojas Arias1Kelly Johanna Ávila Méndez2Silvio Alejandro Lopez-Pazos3Universidad Antonio NariñoFundación Universitaria Agraria de ColombiaUniversidad Antonio NariñoUniversidad Antonio Nariño Batrachochytrium dendrobatidis is a pathogenic fungus causing chytridiomycosis, a cutaneous affection resulting in reduction on Anura populations around the world, because of fungalysins, the most important virulence factor. The B. dendrobatidis maps (http://www.bd-maps.net/) is the dataset of information about the B. dendrobatidis -related isolates. An alternative available for reconstitution of B. dendrobatidis strains from cryopreserved cells include methods relying on the World Organization for Animal Health (OIE) protocol. Most recently, much of the interest in the B. dendrobatidis research has focused on its DNA sequencing, especially B. dendrobatidis JAM81 and B. dendrobatidis JEL423 genomes. OBJECTIVE. To evaluate a modified form from OIE protocol for B. dendrobatidis strain criopreservation, and in silico analysis of B. dendrobatidis fungalysin. OIE protocol was modified using B. dendrobatidis EV001, focused on cryopreservant concentrations, antibiotics and recovering to -80°C, and microscopic viability evaluation on Tryptone Gelatin hydrolyzed agar, besides, Bioinformatics was used for the determination of biochemical characteristics from a B. dendrobatidis JAM81 fungalysin. Modified OIE protocol was useful by viability, recovering B. dendrobatidis EV001 strain. The characterization of B. dendrobatidis JAM81 fungalysin showed a molecular weight of 85 kDa, isoelectric point of 8.33, and tertiary structure among others. This indicated that the protein is a metalloproteinase, it has a PepSY domain for protease inhibition, and a catalytic domain that destroy protein barriers. https://revistas.ups.edu.ec/index.php/granja/article/view/4905Batrachochytrium dendrobatidisanurapathogenzoosporecryopreservationfungalysin |
| spellingShingle | Olga Cristina Lozano Camelo Adriana Carolina Rojas Arias Kelly Johanna Ávila Méndez Silvio Alejandro Lopez-Pazos Modified preservation and fungalysin description for Batrachochytrium dendrobatidis La Granja: Revista de Ciencias de la Vida Batrachochytrium dendrobatidis anura pathogen zoospore cryopreservation fungalysin |
| title | Modified preservation and fungalysin description for Batrachochytrium dendrobatidis |
| title_full | Modified preservation and fungalysin description for Batrachochytrium dendrobatidis |
| title_fullStr | Modified preservation and fungalysin description for Batrachochytrium dendrobatidis |
| title_full_unstemmed | Modified preservation and fungalysin description for Batrachochytrium dendrobatidis |
| title_short | Modified preservation and fungalysin description for Batrachochytrium dendrobatidis |
| title_sort | modified preservation and fungalysin description for batrachochytrium dendrobatidis |
| topic | Batrachochytrium dendrobatidis anura pathogen zoospore cryopreservation fungalysin |
| url | https://revistas.ups.edu.ec/index.php/granja/article/view/4905 |
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