Antioxidant and genoprotective effects of osthole against cadmium-induced DNA damage: an in vitro study using comet assay
Background and purpose: Osthole, a plant-derived coumarin, has shown numerous pharmacological effects. However, its genoprotective effects against cadmium-induced DNA damage have not been determined yet. Therefore, this project aimed to assess the effectiveness of osthole against genotoxicity caused...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Research in Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2022;volume=17;issue=6;spage=657;epage=664;aulast=Vahidifar |
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| author | Ehsan Vahidifar Seyed Ebrahim Sajjadi Mahmoud Etebari |
| author_facet | Ehsan Vahidifar Seyed Ebrahim Sajjadi Mahmoud Etebari |
| author_sort | Ehsan Vahidifar |
| collection | DOAJ |
| description | Background and purpose: Osthole, a plant-derived coumarin, has shown numerous pharmacological effects. However, its genoprotective effects against cadmium-induced DNA damage have not been determined yet. Therefore, this project aimed to assess the effectiveness of osthole against genotoxicity caused by cadmium.
Experimental approach: For this purpose, human umbilical vein endothelial cells (HUVECs) were incubated with various concentrations of osthole (40, 60, 80, and 120 µM) 24 h before cadmium chloride (CdCl2) treatment (40 µM), and then DNA damage was evaluated by comet assay. Furthermore, DPPH and free thiol group assays were applied to evaluate reactive oxygen species scavenger and antioxidant capacities of osthole.
Findings / Results: In the present study, all concentrations of osthole significantly decreased CdCl2-induced DNA damage. Furthermore, the antioxidant properties of the osthole were confirmed by DPPH and free thiol assays.
Conclusion and implications: Overall, the findings of this project revealed that osthole could ameliorate cadmium-induced genotoxicity probably by its antioxidant activity. |
| first_indexed | 2024-04-11T06:43:49Z |
| format | Article |
| id | doaj.art-86a03f7171fb4c54bbc1e4f8a80da2b1 |
| institution | Directory Open Access Journal |
| issn | 1735-5362 1735-9414 |
| language | English |
| last_indexed | 2024-04-11T06:43:49Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Research in Pharmaceutical Sciences |
| spelling | doaj.art-86a03f7171fb4c54bbc1e4f8a80da2b12022-12-22T04:39:24ZengWolters Kluwer Medknow PublicationsResearch in Pharmaceutical Sciences1735-53621735-94142022-01-0117665766410.4103/1735-5362.359432Antioxidant and genoprotective effects of osthole against cadmium-induced DNA damage: an in vitro study using comet assayEhsan VahidifarSeyed Ebrahim SajjadiMahmoud EtebariBackground and purpose: Osthole, a plant-derived coumarin, has shown numerous pharmacological effects. However, its genoprotective effects against cadmium-induced DNA damage have not been determined yet. Therefore, this project aimed to assess the effectiveness of osthole against genotoxicity caused by cadmium. Experimental approach: For this purpose, human umbilical vein endothelial cells (HUVECs) were incubated with various concentrations of osthole (40, 60, 80, and 120 µM) 24 h before cadmium chloride (CdCl2) treatment (40 µM), and then DNA damage was evaluated by comet assay. Furthermore, DPPH and free thiol group assays were applied to evaluate reactive oxygen species scavenger and antioxidant capacities of osthole. Findings / Results: In the present study, all concentrations of osthole significantly decreased CdCl2-induced DNA damage. Furthermore, the antioxidant properties of the osthole were confirmed by DPPH and free thiol assays. Conclusion and implications: Overall, the findings of this project revealed that osthole could ameliorate cadmium-induced genotoxicity probably by its antioxidant activity.http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2022;volume=17;issue=6;spage=657;epage=664;aulast=Vahidifarcadmium; comet assay; dna damage; genotoxicityosthole; oxidative stress. |
| spellingShingle | Ehsan Vahidifar Seyed Ebrahim Sajjadi Mahmoud Etebari Antioxidant and genoprotective effects of osthole against cadmium-induced DNA damage: an in vitro study using comet assay Research in Pharmaceutical Sciences cadmium; comet assay; dna damage; genotoxicity osthole; oxidative stress. |
| title | Antioxidant and genoprotective effects of osthole against cadmium-induced DNA damage: an in vitro study using comet assay |
| title_full | Antioxidant and genoprotective effects of osthole against cadmium-induced DNA damage: an in vitro study using comet assay |
| title_fullStr | Antioxidant and genoprotective effects of osthole against cadmium-induced DNA damage: an in vitro study using comet assay |
| title_full_unstemmed | Antioxidant and genoprotective effects of osthole against cadmium-induced DNA damage: an in vitro study using comet assay |
| title_short | Antioxidant and genoprotective effects of osthole against cadmium-induced DNA damage: an in vitro study using comet assay |
| title_sort | antioxidant and genoprotective effects of osthole against cadmium induced dna damage an in vitro study using comet assay |
| topic | cadmium; comet assay; dna damage; genotoxicity osthole; oxidative stress. |
| url | http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2022;volume=17;issue=6;spage=657;epage=664;aulast=Vahidifar |
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