Evaluation of phage assay for rapid phenotypic detection of rifampicin resistance in <it>Mycobacterium tuberculosis</it>
<p>Abstract</p> <p>Background</p> <p>Conventional methods for susceptibility testing require several months before results can be reported. However, rapid methods to determine drug susceptibility have been developed recently. Phage assay have been reported as a rapid us...
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Format: | Article |
Language: | English |
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BMC
2006-04-01
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Series: | Annals of Clinical Microbiology and Antimicrobials |
Online Access: | http://www.ann-clinmicrob.com/content/5/1/11 |
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author | Montoro Ernesto Echemendia Miguel Lemus Dihadenys Yzquierdo Sergio McNerney Ruth Martin Anandi Palomino Juan |
author_facet | Montoro Ernesto Echemendia Miguel Lemus Dihadenys Yzquierdo Sergio McNerney Ruth Martin Anandi Palomino Juan |
author_sort | Montoro Ernesto |
collection | DOAJ |
description | <p>Abstract</p> <p>Background</p> <p>Conventional methods for susceptibility testing require several months before results can be reported. However, rapid methods to determine drug susceptibility have been developed recently. Phage assay have been reported as a rapid useful tools for antimicrobial susceptibility testing. The aim of this study was to apply the Phage assay for rapid detection of resistance on <it>Mycobacterium tuberculosis </it>strains in Cuba.</p> <p>Methods</p> <p>Phage D29 assay was performed on 102 <it>M. tuberculosis </it>strains to detect rifampicin resistance. The results were compared with the proportion method (gold standard) to evaluate the sensitivity and specificity of Phage assay.</p> <p>Results</p> <p>Phage assay results were available in 2 days whereas Proportion Methods results were obtain in 42 days. A total of 44 strains were detected as rifampicin resistant by both methods. However, one strains deemed resistant by Proportion Methods was susceptible by Phage assay. The sensitivity and specificity of Phage assay were 97.8 % and 100% respectively.</p> <p>Conclusion</p> <p>Phage assay provides rapid and reliable results for susceptibility testing; it's easy to perform, requires no specialized equipment and is applicable to drug susceptibility testing in low income countries where tuberculosis is a major public health problem.</p> |
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issn | 1476-0711 |
language | English |
last_indexed | 2024-04-13T00:39:50Z |
publishDate | 2006-04-01 |
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series | Annals of Clinical Microbiology and Antimicrobials |
spelling | doaj.art-86a13660e9694014a699df20a8647c1a2022-12-22T03:10:13ZengBMCAnnals of Clinical Microbiology and Antimicrobials1476-07112006-04-01511110.1186/1476-0711-5-11Evaluation of phage assay for rapid phenotypic detection of rifampicin resistance in <it>Mycobacterium tuberculosis</it>Montoro ErnestoEchemendia MiguelLemus DihadenysYzquierdo SergioMcNerney RuthMartin AnandiPalomino Juan<p>Abstract</p> <p>Background</p> <p>Conventional methods for susceptibility testing require several months before results can be reported. However, rapid methods to determine drug susceptibility have been developed recently. Phage assay have been reported as a rapid useful tools for antimicrobial susceptibility testing. The aim of this study was to apply the Phage assay for rapid detection of resistance on <it>Mycobacterium tuberculosis </it>strains in Cuba.</p> <p>Methods</p> <p>Phage D29 assay was performed on 102 <it>M. tuberculosis </it>strains to detect rifampicin resistance. The results were compared with the proportion method (gold standard) to evaluate the sensitivity and specificity of Phage assay.</p> <p>Results</p> <p>Phage assay results were available in 2 days whereas Proportion Methods results were obtain in 42 days. A total of 44 strains were detected as rifampicin resistant by both methods. However, one strains deemed resistant by Proportion Methods was susceptible by Phage assay. The sensitivity and specificity of Phage assay were 97.8 % and 100% respectively.</p> <p>Conclusion</p> <p>Phage assay provides rapid and reliable results for susceptibility testing; it's easy to perform, requires no specialized equipment and is applicable to drug susceptibility testing in low income countries where tuberculosis is a major public health problem.</p>http://www.ann-clinmicrob.com/content/5/1/11 |
spellingShingle | Montoro Ernesto Echemendia Miguel Lemus Dihadenys Yzquierdo Sergio McNerney Ruth Martin Anandi Palomino Juan Evaluation of phage assay for rapid phenotypic detection of rifampicin resistance in <it>Mycobacterium tuberculosis</it> Annals of Clinical Microbiology and Antimicrobials |
title | Evaluation of phage assay for rapid phenotypic detection of rifampicin resistance in <it>Mycobacterium tuberculosis</it> |
title_full | Evaluation of phage assay for rapid phenotypic detection of rifampicin resistance in <it>Mycobacterium tuberculosis</it> |
title_fullStr | Evaluation of phage assay for rapid phenotypic detection of rifampicin resistance in <it>Mycobacterium tuberculosis</it> |
title_full_unstemmed | Evaluation of phage assay for rapid phenotypic detection of rifampicin resistance in <it>Mycobacterium tuberculosis</it> |
title_short | Evaluation of phage assay for rapid phenotypic detection of rifampicin resistance in <it>Mycobacterium tuberculosis</it> |
title_sort | evaluation of phage assay for rapid phenotypic detection of rifampicin resistance in it mycobacterium tuberculosis it |
url | http://www.ann-clinmicrob.com/content/5/1/11 |
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