Exploring Bacterial Diversity: How Far Have We Reached?
Many methods have been developed for studying and comparing bacterial diversity. These methods suffer from a number of drawbacks. Culture-dependent methods have a drawback that only a small number of bacteria can be cultured. Although many modifications in the traditional cultivation approach have b...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Sciendo
2023-11-01
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| Series: | Postępy Mikrobiologii |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/am-2023-0011 |
| _version_ | 1797405114775896064 |
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| author | Pandey Himani Lal Devi |
| author_facet | Pandey Himani Lal Devi |
| author_sort | Pandey Himani |
| collection | DOAJ |
| description | Many methods have been developed for studying and comparing bacterial diversity. These methods suffer from a number of drawbacks. Culture-dependent methods have a drawback that only a small number of bacteria can be cultured. Although many modifications in the traditional cultivation approach have been made, such as the use of gellan instead of agar and high throughput dilution to extinction culturing, but a large fraction of microbes still remain uncultured. Culture-independent methods were developed to explore uncultured bacterial diversity but they have their own drawbacks. PCR-based methods have biases during DNA extraction and the removal of substances that may inhibit polymerase activity during PCR and digestion with restriction enzymes. “Omics” approach, i.e., metagenomics, metatranscriptomics, and metaproteomics, aim to link bacterial community structure with function. Different combinations of methods can be used to know more precisely about the bacterial diversity. To date, no known method can reveal the exact bacterial diversity of different environments. This lacuna needs to be filled and newer methods must be developed that can help in exploring the immense bacterial diversity created by nature. |
| first_indexed | 2024-03-09T03:05:05Z |
| format | Article |
| id | doaj.art-86a2571c91ed4511a4e70b3cd7bf2672 |
| institution | Directory Open Access Journal |
| issn | 2545-3149 |
| language | English |
| last_indexed | 2024-03-09T03:05:05Z |
| publishDate | 2023-11-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Postępy Mikrobiologii |
| spelling | doaj.art-86a2571c91ed4511a4e70b3cd7bf26722023-12-04T08:02:51ZengSciendoPostępy Mikrobiologii2545-31492023-11-01623-411713110.2478/am-2023-0011Exploring Bacterial Diversity: How Far Have We Reached?Pandey Himani0Lal Devi1Redcliffe Labs, Electronic City, Noida, Uttar Pradesh201301, India.Ramjas College, University of Delhi, Delhi-110007, India.Many methods have been developed for studying and comparing bacterial diversity. These methods suffer from a number of drawbacks. Culture-dependent methods have a drawback that only a small number of bacteria can be cultured. Although many modifications in the traditional cultivation approach have been made, such as the use of gellan instead of agar and high throughput dilution to extinction culturing, but a large fraction of microbes still remain uncultured. Culture-independent methods were developed to explore uncultured bacterial diversity but they have their own drawbacks. PCR-based methods have biases during DNA extraction and the removal of substances that may inhibit polymerase activity during PCR and digestion with restriction enzymes. “Omics” approach, i.e., metagenomics, metatranscriptomics, and metaproteomics, aim to link bacterial community structure with function. Different combinations of methods can be used to know more precisely about the bacterial diversity. To date, no known method can reveal the exact bacterial diversity of different environments. This lacuna needs to be filled and newer methods must be developed that can help in exploring the immense bacterial diversity created by nature.https://doi.org/10.2478/am-2023-0011bacterial diversityculture-dependent methodsmetagenomicsmetaproteomicsmetatranscriptomicst-rflp |
| spellingShingle | Pandey Himani Lal Devi Exploring Bacterial Diversity: How Far Have We Reached? Postępy Mikrobiologii bacterial diversity culture-dependent methods metagenomics metaproteomics metatranscriptomics t-rflp |
| title | Exploring Bacterial Diversity: How Far Have We Reached? |
| title_full | Exploring Bacterial Diversity: How Far Have We Reached? |
| title_fullStr | Exploring Bacterial Diversity: How Far Have We Reached? |
| title_full_unstemmed | Exploring Bacterial Diversity: How Far Have We Reached? |
| title_short | Exploring Bacterial Diversity: How Far Have We Reached? |
| title_sort | exploring bacterial diversity how far have we reached |
| topic | bacterial diversity culture-dependent methods metagenomics metaproteomics metatranscriptomics t-rflp |
| url | https://doi.org/10.2478/am-2023-0011 |
| work_keys_str_mv | AT pandeyhimani exploringbacterialdiversityhowfarhavewereached AT laldevi exploringbacterialdiversityhowfarhavewereached |