High-throughput sequencing reveals differences in microbial community structure and diversity in the conjunctival tissue of healthy and type 2 diabetic mice
Abstract Background To investigate the differences in bacterial and fungal community structure and diversity in conjunctival tissue of healthy and diabetic mice. Methods RNA-seq assays and high-throughput sequencing of bacterial 16 S rDNA and fungal internal transcribed spacer (ITS) gene sequences w...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-024-03247-y |
| _version_ | 1797259436278939648 |
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| author | Fengjiao Li Shuo Yang Ji Ma Xiaowen Zhao Meng Chen Ye Wang |
| author_facet | Fengjiao Li Shuo Yang Ji Ma Xiaowen Zhao Meng Chen Ye Wang |
| author_sort | Fengjiao Li |
| collection | DOAJ |
| description | Abstract Background To investigate the differences in bacterial and fungal community structure and diversity in conjunctival tissue of healthy and diabetic mice. Methods RNA-seq assays and high-throughput sequencing of bacterial 16 S rDNA and fungal internal transcribed spacer (ITS) gene sequences were used to identify differentially expressed host genes and fungal composition profiles in conjunctival tissues of diabetic BKS-db/db mice and BKS (control) mice. Functional enrichment analysis of differentially expressed genes and the correlation between the relative abundance of bacterial and fungal taxa in the intestinal mucosa were also performed. Results Totally, 449 differential up-regulated genes and 1,006 down-regulated genes were identified in the conjunctival tissues of diabetic mice. The differentially expressed genes were mainly enriched in metabolism-related functions and pathways. A decrease in conjunctival bacterial species diversity and abundance in diabetic mice compared to control mice. In contrast, fungal species richness and diversity were not affected by diabetes. The microbial colonies were mainly associated with cellular process pathways regulating carbohydrate and lipid metabolism, as well as cell growth and death. Additionally, some interactions between bacteria and fungi at different taxonomic levels were also observed. Conclusion The present study revealed significant differences in the abundance and composition of bacterial and fungal communities in the conjunctival tissue of diabetic mice compared to control mice. The study also highlighted interactions between bacteria and fungi at different taxonomic levels. These findings may have implications for the diagnosis and treatment of diabetes. |
| first_indexed | 2024-04-24T23:09:24Z |
| format | Article |
| id | doaj.art-86a3d02bab7d42d3859085359bcb1320 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-24T23:09:24Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-86a3d02bab7d42d3859085359bcb13202024-03-17T12:17:21ZengBMCBMC Microbiology1471-21802024-03-0124111710.1186/s12866-024-03247-yHigh-throughput sequencing reveals differences in microbial community structure and diversity in the conjunctival tissue of healthy and type 2 diabetic miceFengjiao Li0Shuo Yang1Ji Ma2Xiaowen Zhao3Meng Chen4Ye Wang5Department of Opthalmology, Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityEye Center, Second Affiliated Hospital, School of Medicine, Zhejiang UniversityCore Laboratory, The Affiliated Qingdao Central Hospital of Qingdao UniversityCore Laboratory, The Affiliated Qingdao Central Hospital of Qingdao UniversityDepartment of Opthalmology, Qingdao municipal hospital, Qingdao Municipal HospitalCore Laboratory, The Affiliated Qingdao Central Hospital of Qingdao UniversityAbstract Background To investigate the differences in bacterial and fungal community structure and diversity in conjunctival tissue of healthy and diabetic mice. Methods RNA-seq assays and high-throughput sequencing of bacterial 16 S rDNA and fungal internal transcribed spacer (ITS) gene sequences were used to identify differentially expressed host genes and fungal composition profiles in conjunctival tissues of diabetic BKS-db/db mice and BKS (control) mice. Functional enrichment analysis of differentially expressed genes and the correlation between the relative abundance of bacterial and fungal taxa in the intestinal mucosa were also performed. Results Totally, 449 differential up-regulated genes and 1,006 down-regulated genes were identified in the conjunctival tissues of diabetic mice. The differentially expressed genes were mainly enriched in metabolism-related functions and pathways. A decrease in conjunctival bacterial species diversity and abundance in diabetic mice compared to control mice. In contrast, fungal species richness and diversity were not affected by diabetes. The microbial colonies were mainly associated with cellular process pathways regulating carbohydrate and lipid metabolism, as well as cell growth and death. Additionally, some interactions between bacteria and fungi at different taxonomic levels were also observed. Conclusion The present study revealed significant differences in the abundance and composition of bacterial and fungal communities in the conjunctival tissue of diabetic mice compared to control mice. The study also highlighted interactions between bacteria and fungi at different taxonomic levels. These findings may have implications for the diagnosis and treatment of diabetes.https://doi.org/10.1186/s12866-024-03247-yHigh-throughput sequencingDiabetes mellitusConjunctival tissueMicrobial communityDiversity |
| spellingShingle | Fengjiao Li Shuo Yang Ji Ma Xiaowen Zhao Meng Chen Ye Wang High-throughput sequencing reveals differences in microbial community structure and diversity in the conjunctival tissue of healthy and type 2 diabetic mice BMC Microbiology High-throughput sequencing Diabetes mellitus Conjunctival tissue Microbial community Diversity |
| title | High-throughput sequencing reveals differences in microbial community structure and diversity in the conjunctival tissue of healthy and type 2 diabetic mice |
| title_full | High-throughput sequencing reveals differences in microbial community structure and diversity in the conjunctival tissue of healthy and type 2 diabetic mice |
| title_fullStr | High-throughput sequencing reveals differences in microbial community structure and diversity in the conjunctival tissue of healthy and type 2 diabetic mice |
| title_full_unstemmed | High-throughput sequencing reveals differences in microbial community structure and diversity in the conjunctival tissue of healthy and type 2 diabetic mice |
| title_short | High-throughput sequencing reveals differences in microbial community structure and diversity in the conjunctival tissue of healthy and type 2 diabetic mice |
| title_sort | high throughput sequencing reveals differences in microbial community structure and diversity in the conjunctival tissue of healthy and type 2 diabetic mice |
| topic | High-throughput sequencing Diabetes mellitus Conjunctival tissue Microbial community Diversity |
| url | https://doi.org/10.1186/s12866-024-03247-y |
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