A DNA biosensors-based microfluidic platform for attomolar real-time detection of unamplified SARS-CoV-2 virus
The emergence of the coronavirus 2019 (COVID-19) arose the need for rapid, accurate and massive virus detection methods to control the spread of infectious diseases. In this work, a device, deployable in non-medical environments, has been developed for the detection of non-amplified SARS-CoV-2 RNA....
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Biosensors and Bioelectronics: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590137022001959 |
| _version_ | 1797868294006374400 |
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| author | Perrine Robin Laura Barnabei Stefano Marocco Jacopo Pagnoncelli Daniele Nicolis Chiara Tarantelli Agatino Christian Tavilla Roberto Robortella Luciano Cascione Lucas Mayoraz Céline M.A. Journot Mounir Mensi Francesco Bertoni Igor Stefanini Sandrine Gerber-Lemaire |
| author_facet | Perrine Robin Laura Barnabei Stefano Marocco Jacopo Pagnoncelli Daniele Nicolis Chiara Tarantelli Agatino Christian Tavilla Roberto Robortella Luciano Cascione Lucas Mayoraz Céline M.A. Journot Mounir Mensi Francesco Bertoni Igor Stefanini Sandrine Gerber-Lemaire |
| author_sort | Perrine Robin |
| collection | DOAJ |
| description | The emergence of the coronavirus 2019 (COVID-19) arose the need for rapid, accurate and massive virus detection methods to control the spread of infectious diseases. In this work, a device, deployable in non-medical environments, has been developed for the detection of non-amplified SARS-CoV-2 RNA. A SARS-CoV-2 specific probe was designed and covalently immobilized at the surface of glass slides to fabricate a DNA biosensor. The resulting system was integrated in a microfluidic platform, in which viral RNA was extracted from non-treated human saliva, before hybridizing at the surface of the sensor. The formed DNA/RNA duplex was detected in presence of SYBR Green I using an opto-electronic system, based on a high-power LED and a photo multiplier tube, which convert the emitted fluorescence into an electrical signal that can be processed in less than 10 min. The limit of detection of the resulting microfluidic platform reached six copies of viral RNA per microliter of sample (equal to 10 aM) and satisfied the safety margin. The absence of non-specific adsorption and the selectivity for SARS-CoV-2 RNA were established. In addition, the designed device could be applicable for the detection of a variety of viruses by simple modification of the immobilized probe. |
| first_indexed | 2024-04-09T23:53:37Z |
| format | Article |
| id | doaj.art-86a5a012f0024510a57ef47c40de653a |
| institution | Directory Open Access Journal |
| issn | 2590-1370 |
| language | English |
| last_indexed | 2024-04-09T23:53:37Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosensors and Bioelectronics: X |
| spelling | doaj.art-86a5a012f0024510a57ef47c40de653a2023-03-17T04:33:50ZengElsevierBiosensors and Bioelectronics: X2590-13702023-05-0113100302A DNA biosensors-based microfluidic platform for attomolar real-time detection of unamplified SARS-CoV-2 virusPerrine Robin0Laura Barnabei1Stefano Marocco2Jacopo Pagnoncelli3Daniele Nicolis4Chiara Tarantelli5Agatino Christian Tavilla6Roberto Robortella7Luciano Cascione8Lucas Mayoraz9Céline M.A. Journot10Mounir Mensi11Francesco Bertoni12Igor Stefanini13Sandrine Gerber-Lemaire14Group for Functionalized Biomaterials, Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, SwitzerlandInstitute of Oncology Research, Faculty of Biomedical Sciences, USI, Via Francesco Chiesa 5, CH-6500, Bellinzona, SwitzerlandMedical Devices area, Institute of Digital Technologies for Personalized Healthcare - MeDiTech, Department of Innovative Technologies, University of Applied Sciences of Southern Switzerland, Via la Santa 1, CH-6962, Lugano, Viganello, SwitzerlandMedical Devices area, Institute of Digital Technologies for Personalized Healthcare - MeDiTech, Department of Innovative Technologies, University of Applied Sciences of Southern Switzerland, Via la Santa 1, CH-6962, Lugano, Viganello, SwitzerlandDepartment of Innovative Technologies, University of Applied Sciences of Southern Switzerland, Via la Santa 1, CH-6962, Lugano, Viganello, SwitzerlandInstitute of Oncology Research, Faculty of Biomedical Sciences, USI, Via Francesco Chiesa 5, CH-6500, Bellinzona, SwitzerlandDepartment of Innovative Technologies, University of Applied Sciences of Southern Switzerland, Via la Santa 1, CH-6962, Lugano, Viganello, SwitzerlandDepartment of Innovative Technologies, University of Applied Sciences of Southern Switzerland, Via la Santa 1, CH-6962, Lugano, Viganello, SwitzerlandInstitute of Oncology Research, Faculty of Biomedical Sciences, USI, Via Francesco Chiesa 5, CH-6500, Bellinzona, SwitzerlandGroup for Functionalized Biomaterials, Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, SwitzerlandGroup for Functionalized Biomaterials, Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, SwitzerlandISIC-XRDSAP, EPFL Valais-Wallis, Rue de l’Industrie 17, CH-1951, Sion, SwitzerlandInstitute of Oncology Research, Faculty of Biomedical Sciences, USI, Via Francesco Chiesa 5, CH-6500, Bellinzona, Switzerland; Oncology Institute of Southern Switzerland, Ente Ospedaliero Cantonale, CH-6500, Bellinzona, Switzerland; Corresponding author. Institute of Oncology Research, Faculty of Biomedical Sciences, USI, Via Francesco Chiesa 5, CH-6500, Bellinzona, Switzerland.Medical Devices area, Institute of Digital Technologies for Personalized Healthcare - MeDiTech, Department of Innovative Technologies, University of Applied Sciences of Southern Switzerland, Via la Santa 1, CH-6962, Lugano, Viganello, Switzerland; Department of Innovative Technologies, University of Applied Sciences of Southern Switzerland, Via la Santa 1, CH-6962, Lugano, Viganello, Switzerland; Corresponding author. Medical Devices area, Institute of Digital Technologies for Personalized Healthcare - MeDiTech, Department of Innovative Technologies, University of Applied Sciences of Southern Switzerland, Via la Santa 1, CH-6962, Lugano, Viganello, Switzerland.Group for Functionalized Biomaterials, Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland; Corresponding author.The emergence of the coronavirus 2019 (COVID-19) arose the need for rapid, accurate and massive virus detection methods to control the spread of infectious diseases. In this work, a device, deployable in non-medical environments, has been developed for the detection of non-amplified SARS-CoV-2 RNA. A SARS-CoV-2 specific probe was designed and covalently immobilized at the surface of glass slides to fabricate a DNA biosensor. The resulting system was integrated in a microfluidic platform, in which viral RNA was extracted from non-treated human saliva, before hybridizing at the surface of the sensor. The formed DNA/RNA duplex was detected in presence of SYBR Green I using an opto-electronic system, based on a high-power LED and a photo multiplier tube, which convert the emitted fluorescence into an electrical signal that can be processed in less than 10 min. The limit of detection of the resulting microfluidic platform reached six copies of viral RNA per microliter of sample (equal to 10 aM) and satisfied the safety margin. The absence of non-specific adsorption and the selectivity for SARS-CoV-2 RNA were established. In addition, the designed device could be applicable for the detection of a variety of viruses by simple modification of the immobilized probe.http://www.sciencedirect.com/science/article/pii/S2590137022001959DNA-biosensorFluorescenceMicrofluidicRNA extractionSARS-CoV-2 detectionSilica slide |
| spellingShingle | Perrine Robin Laura Barnabei Stefano Marocco Jacopo Pagnoncelli Daniele Nicolis Chiara Tarantelli Agatino Christian Tavilla Roberto Robortella Luciano Cascione Lucas Mayoraz Céline M.A. Journot Mounir Mensi Francesco Bertoni Igor Stefanini Sandrine Gerber-Lemaire A DNA biosensors-based microfluidic platform for attomolar real-time detection of unamplified SARS-CoV-2 virus Biosensors and Bioelectronics: X DNA-biosensor Fluorescence Microfluidic RNA extraction SARS-CoV-2 detection Silica slide |
| title | A DNA biosensors-based microfluidic platform for attomolar real-time detection of unamplified SARS-CoV-2 virus |
| title_full | A DNA biosensors-based microfluidic platform for attomolar real-time detection of unamplified SARS-CoV-2 virus |
| title_fullStr | A DNA biosensors-based microfluidic platform for attomolar real-time detection of unamplified SARS-CoV-2 virus |
| title_full_unstemmed | A DNA biosensors-based microfluidic platform for attomolar real-time detection of unamplified SARS-CoV-2 virus |
| title_short | A DNA biosensors-based microfluidic platform for attomolar real-time detection of unamplified SARS-CoV-2 virus |
| title_sort | dna biosensors based microfluidic platform for attomolar real time detection of unamplified sars cov 2 virus |
| topic | DNA-biosensor Fluorescence Microfluidic RNA extraction SARS-CoV-2 detection Silica slide |
| url | http://www.sciencedirect.com/science/article/pii/S2590137022001959 |
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