Exposure to inorganic arsenic is associated with increased mitochondrial DNA copy number and longer telomere length in peripheral blood.
Background: Exposure to inorganic arsenic (iAs) through drinking water causes cancer. Alterations in mitochondrial DNA copy number (mtDNAcn) and telomere length in blood have been associated with cancer risk. We elucidated if arsenic exposure alters mtDNAcn and telomere length in individuals with di...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2016-08-01
|
| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fcell.2016.00087/full |
| _version_ | 1819195517805002752 |
|---|---|
| author | Syeda Shegufta Ameer Yiyi Xu Karin Engström Huiqi Li Pia Tallving Barbro Nermell Analia Boemo Lidia Guadalupe Peñaloza Luis A. Parada Gabriela Concha Florencia Harari Marie Vahter Karin Broberg |
| author_facet | Syeda Shegufta Ameer Yiyi Xu Karin Engström Huiqi Li Pia Tallving Barbro Nermell Analia Boemo Lidia Guadalupe Peñaloza Luis A. Parada Gabriela Concha Florencia Harari Marie Vahter Karin Broberg |
| author_sort | Syeda Shegufta Ameer |
| collection | DOAJ |
| description | Background: Exposure to inorganic arsenic (iAs) through drinking water causes cancer. Alterations in mitochondrial DNA copy number (mtDNAcn) and telomere length in blood have been associated with cancer risk. We elucidated if arsenic exposure alters mtDNAcn and telomere length in individuals with different arsenic metabolizing capacity.Methods: We studied two groups in the Salta province, Argentina, one in the Puna area of the Andes (N=264, 89% females) and one in Chaco (N=169, 75% females). We assessed arsenic exposure as the sum of arsenic metabolites [iAs, methylarsonic acid (MMA), dimethylarsinic acid (DMA)] in urine (U-As) using high-performance liquid chromatography coupled with hydride generation and inductively coupled plasma mass spectrometry. Efficiency of arsenic metabolism was expressed as percentage of urinary metabolites. MtDNAcn and telomere length were determined in blood by real-time PCR. Results: Median U-As was 196 (5 - 95 percentile: 21 - 537) µg/L in Andes and 80 (5 - 95 percentile: 15 - 1637) µg/L in Chaco. The latter study group had less-efficient metabolism, with higher %iAs and %MMA in urine compared with the Andean group. U-As was significantly associated with increased mtDNAcn (log2 transformed to improve linearity) in Chaco (β=0.027 per 100 µg/L, p=0.0085; adjusted for age and sex), but not in Andes (β=0.025, p=0.24). U-As was also associated with longer telomere length in Chaco (β=0.016, p=0.0066) and Andes (β=0.0075, p=0.029). In both populations, individuals with above median %iAs showed significantly higher mtDNAcn and telomere length compared with individuals with below median %iAs. Conclusions: Arsenic was associated with increased mtDNAcn and telomere length, particularly in individuals with less-efficient arsenic metabolism, a group who may have increased risk for arsenic-related cancer. |
| first_indexed | 2024-12-23T02:14:01Z |
| format | Article |
| id | doaj.art-86a7705405864cc0ac1f40d1ce9d3313 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-23T02:14:01Z |
| publishDate | 2016-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-86a7705405864cc0ac1f40d1ce9d33132022-12-21T18:03:42ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2016-08-01410.3389/fcell.2016.00087196830Exposure to inorganic arsenic is associated with increased mitochondrial DNA copy number and longer telomere length in peripheral blood.Syeda Shegufta Ameer0Yiyi Xu1Karin Engström2Huiqi Li3Pia Tallving4Barbro Nermell5Analia Boemo6Lidia Guadalupe Peñaloza7Luis A. Parada8Gabriela Concha9Florencia Harari10Marie Vahter11Karin Broberg12Lund UniversityLund UniversityKarolinska InstitutetLund UniversityLund UniversityKarolinska InstitutetUniversidad Nacional de SaltaUniversidad Nacional de SaltaUNSa - CONICETNational Food AgencyKarolinska InstitutetKarolinska InstitutetKarolinska InstitutetBackground: Exposure to inorganic arsenic (iAs) through drinking water causes cancer. Alterations in mitochondrial DNA copy number (mtDNAcn) and telomere length in blood have been associated with cancer risk. We elucidated if arsenic exposure alters mtDNAcn and telomere length in individuals with different arsenic metabolizing capacity.Methods: We studied two groups in the Salta province, Argentina, one in the Puna area of the Andes (N=264, 89% females) and one in Chaco (N=169, 75% females). We assessed arsenic exposure as the sum of arsenic metabolites [iAs, methylarsonic acid (MMA), dimethylarsinic acid (DMA)] in urine (U-As) using high-performance liquid chromatography coupled with hydride generation and inductively coupled plasma mass spectrometry. Efficiency of arsenic metabolism was expressed as percentage of urinary metabolites. MtDNAcn and telomere length were determined in blood by real-time PCR. Results: Median U-As was 196 (5 - 95 percentile: 21 - 537) µg/L in Andes and 80 (5 - 95 percentile: 15 - 1637) µg/L in Chaco. The latter study group had less-efficient metabolism, with higher %iAs and %MMA in urine compared with the Andean group. U-As was significantly associated with increased mtDNAcn (log2 transformed to improve linearity) in Chaco (β=0.027 per 100 µg/L, p=0.0085; adjusted for age and sex), but not in Andes (β=0.025, p=0.24). U-As was also associated with longer telomere length in Chaco (β=0.016, p=0.0066) and Andes (β=0.0075, p=0.029). In both populations, individuals with above median %iAs showed significantly higher mtDNAcn and telomere length compared with individuals with below median %iAs. Conclusions: Arsenic was associated with increased mtDNAcn and telomere length, particularly in individuals with less-efficient arsenic metabolism, a group who may have increased risk for arsenic-related cancer.http://journal.frontiersin.org/Journal/10.3389/fcell.2016.00087/fullOxidative PhosphorylationCancerEnergyMMAAs3MT |
| spellingShingle | Syeda Shegufta Ameer Yiyi Xu Karin Engström Huiqi Li Pia Tallving Barbro Nermell Analia Boemo Lidia Guadalupe Peñaloza Luis A. Parada Gabriela Concha Florencia Harari Marie Vahter Karin Broberg Exposure to inorganic arsenic is associated with increased mitochondrial DNA copy number and longer telomere length in peripheral blood. Frontiers in Cell and Developmental Biology Oxidative Phosphorylation Cancer Energy MMA As3MT |
| title | Exposure to inorganic arsenic is associated with increased mitochondrial DNA copy number and longer telomere length in peripheral blood. |
| title_full | Exposure to inorganic arsenic is associated with increased mitochondrial DNA copy number and longer telomere length in peripheral blood. |
| title_fullStr | Exposure to inorganic arsenic is associated with increased mitochondrial DNA copy number and longer telomere length in peripheral blood. |
| title_full_unstemmed | Exposure to inorganic arsenic is associated with increased mitochondrial DNA copy number and longer telomere length in peripheral blood. |
| title_short | Exposure to inorganic arsenic is associated with increased mitochondrial DNA copy number and longer telomere length in peripheral blood. |
| title_sort | exposure to inorganic arsenic is associated with increased mitochondrial dna copy number and longer telomere length in peripheral blood |
| topic | Oxidative Phosphorylation Cancer Energy MMA As3MT |
| url | http://journal.frontiersin.org/Journal/10.3389/fcell.2016.00087/full |
| work_keys_str_mv | AT syedasheguftaameer exposuretoinorganicarsenicisassociatedwithincreasedmitochondrialdnacopynumberandlongertelomerelengthinperipheralblood AT yiyixu exposuretoinorganicarsenicisassociatedwithincreasedmitochondrialdnacopynumberandlongertelomerelengthinperipheralblood AT karinengstrom exposuretoinorganicarsenicisassociatedwithincreasedmitochondrialdnacopynumberandlongertelomerelengthinperipheralblood AT huiqili exposuretoinorganicarsenicisassociatedwithincreasedmitochondrialdnacopynumberandlongertelomerelengthinperipheralblood AT piatallving exposuretoinorganicarsenicisassociatedwithincreasedmitochondrialdnacopynumberandlongertelomerelengthinperipheralblood AT barbronermell exposuretoinorganicarsenicisassociatedwithincreasedmitochondrialdnacopynumberandlongertelomerelengthinperipheralblood AT analiaboemo exposuretoinorganicarsenicisassociatedwithincreasedmitochondrialdnacopynumberandlongertelomerelengthinperipheralblood AT lidiaguadalupepenaloza exposuretoinorganicarsenicisassociatedwithincreasedmitochondrialdnacopynumberandlongertelomerelengthinperipheralblood AT luisaparada exposuretoinorganicarsenicisassociatedwithincreasedmitochondrialdnacopynumberandlongertelomerelengthinperipheralblood AT gabrielaconcha exposuretoinorganicarsenicisassociatedwithincreasedmitochondrialdnacopynumberandlongertelomerelengthinperipheralblood AT florenciaharari exposuretoinorganicarsenicisassociatedwithincreasedmitochondrialdnacopynumberandlongertelomerelengthinperipheralblood AT marievahter exposuretoinorganicarsenicisassociatedwithincreasedmitochondrialdnacopynumberandlongertelomerelengthinperipheralblood AT karinbroberg exposuretoinorganicarsenicisassociatedwithincreasedmitochondrialdnacopynumberandlongertelomerelengthinperipheralblood |