Potential of Exogenous Treatment with Dehydroascorbate to Control Root-knot Nematode Infection in Rice
Abstract Induced resistance (IR) is a unique physiological state characterized by reduced plant susceptibility to (a)biotic stress. Our previous studies showed that exogenous foliar application of dehydroascorbate (DHA), the oxidized form of ascorbic acid, induces systemic resistance against root-kn...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-06-01
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| Series: | Rice |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12284-023-00644-1 |
| _version_ | 1827910568393048064 |
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| author | Satish Namdeo Chavan Farzana Haque Tumpa Md. Atiqur Rahman Khokon Tina Kyndt |
| author_facet | Satish Namdeo Chavan Farzana Haque Tumpa Md. Atiqur Rahman Khokon Tina Kyndt |
| author_sort | Satish Namdeo Chavan |
| collection | DOAJ |
| description | Abstract Induced resistance (IR) is a unique physiological state characterized by reduced plant susceptibility to (a)biotic stress. Our previous studies showed that exogenous foliar application of dehydroascorbate (DHA), the oxidized form of ascorbic acid, induces systemic resistance against root-knot nematode Meloidogyne graminicola in rice. In the present study, the potential of DHA in protecting rice plants against M. graminicola was evaluated in lab, pot, and field studies. In an experiment where the interval between foliar treatment and inoculation was varied, 20 mM DHA was found to protect rice plants from M. graminicola for at least 14 days. Pot and field studies confirmed that 10 or 20 mM DHA are highly effective in reducing gall formation and led to a significant increase in rice seed yield. A half dose of DHA (10 mM) combined with another IR-stimulus - piperonylic acid (PA) 300 µM - was at par with DHA 20 mM, leading to reductions in gall formation of more than 80%. In in vitro bioassays, DHA was found to be highly nematicidal to the second-stage juveniles of M. graminicola, with more than 90% mortality within 3 h of exposure to 10 or 20 mM concentrations. While seed treatment had no effect, root drenching or root dipping was also effective in reducing rice susceptibility to M. graminicola, next to foliar treatment. As a dual-action compound with extended protection and ease of application, DHA has great potential for effective nematode management in rice. |
| first_indexed | 2024-03-13T01:52:36Z |
| format | Article |
| id | doaj.art-86afc12f98ee416baa105497af539fae |
| institution | Directory Open Access Journal |
| issn | 1939-8425 1939-8433 |
| language | English |
| last_indexed | 2024-03-13T01:52:36Z |
| publishDate | 2023-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Rice |
| spelling | doaj.art-86afc12f98ee416baa105497af539fae2023-07-02T11:26:58ZengSpringerOpenRice1939-84251939-84332023-06-0116111610.1186/s12284-023-00644-1Potential of Exogenous Treatment with Dehydroascorbate to Control Root-knot Nematode Infection in RiceSatish Namdeo Chavan0Farzana Haque Tumpa1Md. Atiqur Rahman Khokon2Tina Kyndt3Department of Biotechnology, Faculty of Bioscience Engineering, Ghent UniversityDepartment of Biotechnology, Faculty of Bioscience Engineering, Ghent UniversityDepartment of Plant Pathology, Bangladesh Agricultural UniversityDepartment of Biotechnology, Faculty of Bioscience Engineering, Ghent UniversityAbstract Induced resistance (IR) is a unique physiological state characterized by reduced plant susceptibility to (a)biotic stress. Our previous studies showed that exogenous foliar application of dehydroascorbate (DHA), the oxidized form of ascorbic acid, induces systemic resistance against root-knot nematode Meloidogyne graminicola in rice. In the present study, the potential of DHA in protecting rice plants against M. graminicola was evaluated in lab, pot, and field studies. In an experiment where the interval between foliar treatment and inoculation was varied, 20 mM DHA was found to protect rice plants from M. graminicola for at least 14 days. Pot and field studies confirmed that 10 or 20 mM DHA are highly effective in reducing gall formation and led to a significant increase in rice seed yield. A half dose of DHA (10 mM) combined with another IR-stimulus - piperonylic acid (PA) 300 µM - was at par with DHA 20 mM, leading to reductions in gall formation of more than 80%. In in vitro bioassays, DHA was found to be highly nematicidal to the second-stage juveniles of M. graminicola, with more than 90% mortality within 3 h of exposure to 10 or 20 mM concentrations. While seed treatment had no effect, root drenching or root dipping was also effective in reducing rice susceptibility to M. graminicola, next to foliar treatment. As a dual-action compound with extended protection and ease of application, DHA has great potential for effective nematode management in rice.https://doi.org/10.1186/s12284-023-00644-1DehydroascorbateInduced resistanceIntegrated pest managementMeloidogyne graminicolaNematicidalRice |
| spellingShingle | Satish Namdeo Chavan Farzana Haque Tumpa Md. Atiqur Rahman Khokon Tina Kyndt Potential of Exogenous Treatment with Dehydroascorbate to Control Root-knot Nematode Infection in Rice Rice Dehydroascorbate Induced resistance Integrated pest management Meloidogyne graminicola Nematicidal Rice |
| title | Potential of Exogenous Treatment with Dehydroascorbate to Control Root-knot Nematode Infection in Rice |
| title_full | Potential of Exogenous Treatment with Dehydroascorbate to Control Root-knot Nematode Infection in Rice |
| title_fullStr | Potential of Exogenous Treatment with Dehydroascorbate to Control Root-knot Nematode Infection in Rice |
| title_full_unstemmed | Potential of Exogenous Treatment with Dehydroascorbate to Control Root-knot Nematode Infection in Rice |
| title_short | Potential of Exogenous Treatment with Dehydroascorbate to Control Root-knot Nematode Infection in Rice |
| title_sort | potential of exogenous treatment with dehydroascorbate to control root knot nematode infection in rice |
| topic | Dehydroascorbate Induced resistance Integrated pest management Meloidogyne graminicola Nematicidal Rice |
| url | https://doi.org/10.1186/s12284-023-00644-1 |
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