Enhanced Recovery of Antioxidant Compounds from Hazelnut (<i>Corylus avellana</i> L.) Involucre Based on Extraction Optimization: Phytochemical Profile and Biological Activities
Tree nut by-products could contain a wide range of phytochemicals, natural antioxidants, which might be used as a natural source for dietary supplements. The aim of the present study was to evaluate the phenolic and sterolic composition, as well as the antioxidant and other biological activities, of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-10-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/8/10/460 |
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| author | Marius Emil Rusu Ionel Fizeșan Anca Pop Ana-Maria Gheldiu Andrei Mocan Gianina Crișan Laurian Vlase Felicia Loghin Daniela-Saveta Popa Ioan Tomuta |
| author_facet | Marius Emil Rusu Ionel Fizeșan Anca Pop Ana-Maria Gheldiu Andrei Mocan Gianina Crișan Laurian Vlase Felicia Loghin Daniela-Saveta Popa Ioan Tomuta |
| author_sort | Marius Emil Rusu |
| collection | DOAJ |
| description | Tree nut by-products could contain a wide range of phytochemicals, natural antioxidants, which might be used as a natural source for dietary supplements. The aim of the present study was to evaluate the phenolic and sterolic composition, as well as the antioxidant and other biological activities, of hazelnut involucre (HI) extracts. Experimental designs were developed in order to select the optimum extraction conditions (solvent, temperature, time) using turbo-extraction by Ultra-Turrax for obtaining extracts rich in bioactive compounds. Qualitative and quantitative analyses were performed by LC-MS and LC-MS/MS and they revealed important amounts of individual polyphenols and phytosterols, molecules with antioxidant potential. The richest polyphenolic HI extract with the highest antioxidant activity by TEAC assay was further evaluated by other in vitro antioxidant tests (DPPH, FRAP) and enzyme inhibitory assays. Additionally, the cytotoxic and antioxidant effects of this extract on two cancerous cell lines and on normal cells were tested. This is the first study to analyze the composition of both hydrophilic and lipophilic bioactive compounds in HI extracts. Our findings reveal that this plant by-product presents strong biological activities, justifying further research, and it could be considered an inexpensive source of natural antioxidants for food, pharmaceutical, or cosmetic industry. |
| first_indexed | 2024-03-12T09:39:14Z |
| format | Article |
| id | doaj.art-86b49bb3dc4747ed9c97bf3f05d26e9b |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T09:39:14Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-86b49bb3dc4747ed9c97bf3f05d26e9b2023-09-02T13:21:56ZengMDPI AGAntioxidants2076-39212019-10-0181046010.3390/antiox8100460antiox8100460Enhanced Recovery of Antioxidant Compounds from Hazelnut (<i>Corylus avellana</i> L.) Involucre Based on Extraction Optimization: Phytochemical Profile and Biological ActivitiesMarius Emil Rusu0Ionel Fizeșan1Anca Pop2Ana-Maria Gheldiu3Andrei Mocan4Gianina Crișan5Laurian Vlase6Felicia Loghin7Daniela-Saveta Popa8Ioan Tomuta9Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, “Iuliu Hatieganu” University of Medicine and Pharmacy, 8 Victor Babes, 400012 Cluj-Napoca, RomaniaDepartment of Toxicology, Faculty of Pharmacy, “Iuliu Hatieganu” University of Medicine and Pharmacy, 8 Victor Babes, 400012 Cluj-Napoca, RomaniaDepartment of Toxicology, Faculty of Pharmacy, “Iuliu Hatieganu” University of Medicine and Pharmacy, 8 Victor Babes, 400012 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Botany, Faculty of Pharmacy, “Iuliu Hatieganu” University of Medicine and Pharmacy, 8 Victor Babes, 400012 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Botany, Faculty of Pharmacy, “Iuliu Hatieganu” University of Medicine and Pharmacy, 8 Victor Babes, 400012 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Botany, Faculty of Pharmacy, “Iuliu Hatieganu” University of Medicine and Pharmacy, 8 Victor Babes, 400012 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, “Iuliu Hatieganu” University of Medicine and Pharmacy, 8 Victor Babes, 400012 Cluj-Napoca, RomaniaDepartment of Toxicology, Faculty of Pharmacy, “Iuliu Hatieganu” University of Medicine and Pharmacy, 8 Victor Babes, 400012 Cluj-Napoca, RomaniaDepartment of Toxicology, Faculty of Pharmacy, “Iuliu Hatieganu” University of Medicine and Pharmacy, 8 Victor Babes, 400012 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, “Iuliu Hatieganu” University of Medicine and Pharmacy, 8 Victor Babes, 400012 Cluj-Napoca, RomaniaTree nut by-products could contain a wide range of phytochemicals, natural antioxidants, which might be used as a natural source for dietary supplements. The aim of the present study was to evaluate the phenolic and sterolic composition, as well as the antioxidant and other biological activities, of hazelnut involucre (HI) extracts. Experimental designs were developed in order to select the optimum extraction conditions (solvent, temperature, time) using turbo-extraction by Ultra-Turrax for obtaining extracts rich in bioactive compounds. Qualitative and quantitative analyses were performed by LC-MS and LC-MS/MS and they revealed important amounts of individual polyphenols and phytosterols, molecules with antioxidant potential. The richest polyphenolic HI extract with the highest antioxidant activity by TEAC assay was further evaluated by other in vitro antioxidant tests (DPPH, FRAP) and enzyme inhibitory assays. Additionally, the cytotoxic and antioxidant effects of this extract on two cancerous cell lines and on normal cells were tested. This is the first study to analyze the composition of both hydrophilic and lipophilic bioactive compounds in HI extracts. Our findings reveal that this plant by-product presents strong biological activities, justifying further research, and it could be considered an inexpensive source of natural antioxidants for food, pharmaceutical, or cosmetic industry.https://www.mdpi.com/2076-3921/8/10/460hazelnut involucreantioxidantspolyphenolsphytosterolsbiological activityexperimental designlc-msturbo-extractionultra-turraxenzymatic inhibition |
| spellingShingle | Marius Emil Rusu Ionel Fizeșan Anca Pop Ana-Maria Gheldiu Andrei Mocan Gianina Crișan Laurian Vlase Felicia Loghin Daniela-Saveta Popa Ioan Tomuta Enhanced Recovery of Antioxidant Compounds from Hazelnut (<i>Corylus avellana</i> L.) Involucre Based on Extraction Optimization: Phytochemical Profile and Biological Activities Antioxidants hazelnut involucre antioxidants polyphenols phytosterols biological activity experimental design lc-ms turbo-extraction ultra-turrax enzymatic inhibition |
| title | Enhanced Recovery of Antioxidant Compounds from Hazelnut (<i>Corylus avellana</i> L.) Involucre Based on Extraction Optimization: Phytochemical Profile and Biological Activities |
| title_full | Enhanced Recovery of Antioxidant Compounds from Hazelnut (<i>Corylus avellana</i> L.) Involucre Based on Extraction Optimization: Phytochemical Profile and Biological Activities |
| title_fullStr | Enhanced Recovery of Antioxidant Compounds from Hazelnut (<i>Corylus avellana</i> L.) Involucre Based on Extraction Optimization: Phytochemical Profile and Biological Activities |
| title_full_unstemmed | Enhanced Recovery of Antioxidant Compounds from Hazelnut (<i>Corylus avellana</i> L.) Involucre Based on Extraction Optimization: Phytochemical Profile and Biological Activities |
| title_short | Enhanced Recovery of Antioxidant Compounds from Hazelnut (<i>Corylus avellana</i> L.) Involucre Based on Extraction Optimization: Phytochemical Profile and Biological Activities |
| title_sort | enhanced recovery of antioxidant compounds from hazelnut i corylus avellana i l involucre based on extraction optimization phytochemical profile and biological activities |
| topic | hazelnut involucre antioxidants polyphenols phytosterols biological activity experimental design lc-ms turbo-extraction ultra-turrax enzymatic inhibition |
| url | https://www.mdpi.com/2076-3921/8/10/460 |
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