Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay
<p>Abstract</p> <p>Understanding the mechanisms that maintain protective antibody levels after immunisation is important for vaccine design. In this study, we have determined the kinetics of plasma and memory B cells detectable in the blood of cattle immunised with model T-dependen...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-10-01
|
| Series: | Veterinary Research |
| Online Access: | http://www.veterinaryresearch.org/content/43/1/68 |
| _version_ | 1818564559925936128 |
|---|---|
| author | Grant Clare FJ Lefevre Eric A Carr B Prentice Helen Gubbins Simon Pollard Andrew J Charreyre Catherine Charleston Bryan |
| author_facet | Grant Clare FJ Lefevre Eric A Carr B Prentice Helen Gubbins Simon Pollard Andrew J Charreyre Catherine Charleston Bryan |
| author_sort | Grant Clare FJ |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Understanding the mechanisms that maintain protective antibody levels after immunisation is important for vaccine design. In this study, we have determined the kinetics of plasma and memory B cells detectable in the blood of cattle immunised with model T-dependent or T-independent antigens. Immunisation with the T-D antigen resulted in an expansion of TNP-specific plasma cells post-TNP primary and booster immunisations, which was associated with increased titres of TNP-specific IgG antibodies. Although no TNP-specific memory B cells were detected in the T-D group following the primary immunisation, we detected an increase in the number of TNP-specific memory B cells post-TNP boost. In contrast, no TNP-specific plasma or memory B cells were detected after primary or secondary immunisation with the T-I antigen. We then investigated if immunisation with a third party antigen (tetanus toxin fragment C, TTC) would result in a bystander stimulation and increase the number of TNP-specific plasma and memory B cells in the T-D and/or T-I group. TTC immunisation in the T-D group resulted in a small increase in the number of TNP-specific plasma cells post-TTC primary immunisation and boost, and in an increase in the number of TNP-specific memory B cells post-TTC boost. This bystander effect was not observed in the animals previously immunised with the T-I antigen. In conclusion, the present study characterised for the first time the B cell response in cattle to immunisation with T-D and T-I antigens and showed that bystander stimulation of an established T-D B cell memory response may occur in cattle.</p> |
| first_indexed | 2024-12-14T01:30:25Z |
| format | Article |
| id | doaj.art-86b98d9efb0f44a09514e9fd531e7070 |
| institution | Directory Open Access Journal |
| issn | 0928-4249 1297-9716 |
| language | English |
| last_indexed | 2024-12-14T01:30:25Z |
| publishDate | 2012-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-86b98d9efb0f44a09514e9fd531e70702022-12-21T23:22:03ZengBMCVeterinary Research0928-42491297-97162012-10-014316810.1186/1297-9716-43-68Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assayGrant Clare FJLefevre Eric ACarr BPrentice HelenGubbins SimonPollard Andrew JCharreyre CatherineCharleston Bryan<p>Abstract</p> <p>Understanding the mechanisms that maintain protective antibody levels after immunisation is important for vaccine design. In this study, we have determined the kinetics of plasma and memory B cells detectable in the blood of cattle immunised with model T-dependent or T-independent antigens. Immunisation with the T-D antigen resulted in an expansion of TNP-specific plasma cells post-TNP primary and booster immunisations, which was associated with increased titres of TNP-specific IgG antibodies. Although no TNP-specific memory B cells were detected in the T-D group following the primary immunisation, we detected an increase in the number of TNP-specific memory B cells post-TNP boost. In contrast, no TNP-specific plasma or memory B cells were detected after primary or secondary immunisation with the T-I antigen. We then investigated if immunisation with a third party antigen (tetanus toxin fragment C, TTC) would result in a bystander stimulation and increase the number of TNP-specific plasma and memory B cells in the T-D and/or T-I group. TTC immunisation in the T-D group resulted in a small increase in the number of TNP-specific plasma cells post-TTC primary immunisation and boost, and in an increase in the number of TNP-specific memory B cells post-TTC boost. This bystander effect was not observed in the animals previously immunised with the T-I antigen. In conclusion, the present study characterised for the first time the B cell response in cattle to immunisation with T-D and T-I antigens and showed that bystander stimulation of an established T-D B cell memory response may occur in cattle.</p>http://www.veterinaryresearch.org/content/43/1/68 |
| spellingShingle | Grant Clare FJ Lefevre Eric A Carr B Prentice Helen Gubbins Simon Pollard Andrew J Charreyre Catherine Charleston Bryan Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay Veterinary Research |
| title | Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay |
| title_full | Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay |
| title_fullStr | Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay |
| title_full_unstemmed | Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay |
| title_short | Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay |
| title_sort | assessment of t dependent and t independent immune responses in cattle using a b cell elispot assay |
| url | http://www.veterinaryresearch.org/content/43/1/68 |
| work_keys_str_mv | AT grantclarefj assessmentoftdependentandtindependentimmuneresponsesincattleusingabcellelispotassay AT lefevreerica assessmentoftdependentandtindependentimmuneresponsesincattleusingabcellelispotassay AT carrb assessmentoftdependentandtindependentimmuneresponsesincattleusingabcellelispotassay AT prenticehelen assessmentoftdependentandtindependentimmuneresponsesincattleusingabcellelispotassay AT gubbinssimon assessmentoftdependentandtindependentimmuneresponsesincattleusingabcellelispotassay AT pollardandrewj assessmentoftdependentandtindependentimmuneresponsesincattleusingabcellelispotassay AT charreyrecatherine assessmentoftdependentandtindependentimmuneresponsesincattleusingabcellelispotassay AT charlestonbryan assessmentoftdependentandtindependentimmuneresponsesincattleusingabcellelispotassay |