Metabolic Response of <i>Faecalibacterium prausnitzii</i> to Cell-Free Supernatants from Lactic Acid Bacteria
Interest in preventive or therapeutic strategies targeting gut microbiota is increasing. Such strategies may involve the direct replenishment of the gut microbiota with single strains or strain mixtures, or the manipulation of strain abundance through dietary intervention, including lactic acid bact...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-10-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/8/10/1528 |
| _version_ | 1797551780131766272 |
|---|---|
| author | Mathilde Lebas Peggy Garault Daniel Carrillo Francisco M. Codoñer Muriel Derrien |
| author_facet | Mathilde Lebas Peggy Garault Daniel Carrillo Francisco M. Codoñer Muriel Derrien |
| author_sort | Mathilde Lebas |
| collection | DOAJ |
| description | Interest in preventive or therapeutic strategies targeting gut microbiota is increasing. Such strategies may involve the direct replenishment of the gut microbiota with single strains or strain mixtures, or the manipulation of strain abundance through dietary intervention, including lactic acid bacteria. A few candidate species associated with health benefits have been identified, including <i>Faecalibacterium prausnitzii</i>. Given its growth requirements, modulation of this bacterium has not been extensively studied. In this investigation, we explored the capacity of cell-free supernatants of different <i>Lactobacillus</i>, <i>Streptococcus</i>, <i>Lactococcus,</i> and <i>Bifidobacterium</i> strains to stimulate the growth of <i>F. prausnitzii</i> A2-165. Modulation by four strains with the greatest capacity to stimulate growth or delay lysis, <i>Lactococcus lactis</i> subsp. lactis CNCM I-1631, <i>Lactococcus lactis</i> subsp. <i>cremoris</i> CNCM I-3558, <i>Lactobacillus paracasei</i> CNCM I-3689, and <i>Streptococcus thermophilus</i> CNCM I-3862, was further characterized by transcriptomics. The response of <i>F. prausnitzii</i> to cell-free supernatants from these four strains revealed several shared characteristics, in particular, upregulation of carbohydrate metabolism and cell wall-related genes and downregulation of replication and mobilome genes. Overall, this study suggests differential responses of <i>F. prausnitzii</i> to metabolites produced by different strains, providing protection against cell death, with an increase in peptidoglycan levels for cell wall formation, and reduced cell mobilome activity. |
| first_indexed | 2024-03-10T15:49:53Z |
| format | Article |
| id | doaj.art-86bba6db89ba45a882dce1642bb9aacb |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T15:49:53Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-86bba6db89ba45a882dce1642bb9aacb2023-11-20T16:06:45ZengMDPI AGMicroorganisms2076-26072020-10-01810152810.3390/microorganisms8101528Metabolic Response of <i>Faecalibacterium prausnitzii</i> to Cell-Free Supernatants from Lactic Acid BacteriaMathilde Lebas0Peggy Garault1Daniel Carrillo2Francisco M. Codoñer3Muriel Derrien4Danone Nutricia Research, RD 128 Avenue de la Vauve, 91767 Palaiseau Cédex, FranceDanone Nutricia Research, RD 128 Avenue de la Vauve, 91767 Palaiseau Cédex, FranceArcher Daniels Midland Co-Biopolis ADM Nutrition, C/Catedratico Agustin Escardino num 9 Edif 2, Paterna, 46980 Valencia, SpainArcher Daniels Midland Co-Biopolis ADM Nutrition, C/Catedratico Agustin Escardino num 9 Edif 2, Paterna, 46980 Valencia, SpainDanone Nutricia Research, RD 128 Avenue de la Vauve, 91767 Palaiseau Cédex, FranceInterest in preventive or therapeutic strategies targeting gut microbiota is increasing. Such strategies may involve the direct replenishment of the gut microbiota with single strains or strain mixtures, or the manipulation of strain abundance through dietary intervention, including lactic acid bacteria. A few candidate species associated with health benefits have been identified, including <i>Faecalibacterium prausnitzii</i>. Given its growth requirements, modulation of this bacterium has not been extensively studied. In this investigation, we explored the capacity of cell-free supernatants of different <i>Lactobacillus</i>, <i>Streptococcus</i>, <i>Lactococcus,</i> and <i>Bifidobacterium</i> strains to stimulate the growth of <i>F. prausnitzii</i> A2-165. Modulation by four strains with the greatest capacity to stimulate growth or delay lysis, <i>Lactococcus lactis</i> subsp. lactis CNCM I-1631, <i>Lactococcus lactis</i> subsp. <i>cremoris</i> CNCM I-3558, <i>Lactobacillus paracasei</i> CNCM I-3689, and <i>Streptococcus thermophilus</i> CNCM I-3862, was further characterized by transcriptomics. The response of <i>F. prausnitzii</i> to cell-free supernatants from these four strains revealed several shared characteristics, in particular, upregulation of carbohydrate metabolism and cell wall-related genes and downregulation of replication and mobilome genes. Overall, this study suggests differential responses of <i>F. prausnitzii</i> to metabolites produced by different strains, providing protection against cell death, with an increase in peptidoglycan levels for cell wall formation, and reduced cell mobilome activity.https://www.mdpi.com/2076-2607/8/10/1528<i>Faecalibacterium prausnitzii</i>lactic acid bacterialysisin vitro |
| spellingShingle | Mathilde Lebas Peggy Garault Daniel Carrillo Francisco M. Codoñer Muriel Derrien Metabolic Response of <i>Faecalibacterium prausnitzii</i> to Cell-Free Supernatants from Lactic Acid Bacteria Microorganisms <i>Faecalibacterium prausnitzii</i> lactic acid bacteria lysis in vitro |
| title | Metabolic Response of <i>Faecalibacterium prausnitzii</i> to Cell-Free Supernatants from Lactic Acid Bacteria |
| title_full | Metabolic Response of <i>Faecalibacterium prausnitzii</i> to Cell-Free Supernatants from Lactic Acid Bacteria |
| title_fullStr | Metabolic Response of <i>Faecalibacterium prausnitzii</i> to Cell-Free Supernatants from Lactic Acid Bacteria |
| title_full_unstemmed | Metabolic Response of <i>Faecalibacterium prausnitzii</i> to Cell-Free Supernatants from Lactic Acid Bacteria |
| title_short | Metabolic Response of <i>Faecalibacterium prausnitzii</i> to Cell-Free Supernatants from Lactic Acid Bacteria |
| title_sort | metabolic response of i faecalibacterium prausnitzii i to cell free supernatants from lactic acid bacteria |
| topic | <i>Faecalibacterium prausnitzii</i> lactic acid bacteria lysis in vitro |
| url | https://www.mdpi.com/2076-2607/8/10/1528 |
| work_keys_str_mv | AT mathildelebas metabolicresponseofifaecalibacteriumprausnitziiitocellfreesupernatantsfromlacticacidbacteria AT peggygarault metabolicresponseofifaecalibacteriumprausnitziiitocellfreesupernatantsfromlacticacidbacteria AT danielcarrillo metabolicresponseofifaecalibacteriumprausnitziiitocellfreesupernatantsfromlacticacidbacteria AT franciscomcodoner metabolicresponseofifaecalibacteriumprausnitziiitocellfreesupernatantsfromlacticacidbacteria AT murielderrien metabolicresponseofifaecalibacteriumprausnitziiitocellfreesupernatantsfromlacticacidbacteria |