Characterization and gene expression patterns analysis implies BSK family genes respond to salinity stress in cotton
Identification, evolution, and expression patterns of BSK (BR signaling kinase) family genes revealed that BSKs participated in the response of cotton to abiotic stress and maintained the growth of cotton in extreme environment. The steroidal hormone brassinosteroids (BR) play important roles in dif...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2023.1169104/full |
| _version_ | 1797809788129640448 |
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| author | Yuqian Lei Yuqian Lei Yupeng Cui Ruifeng Cui Xiugui Chen Junjuan Wang Xuke Lu Delong Wang Shuai Wang Lixue Guo Yuexin Zhang Cun Rui Yapeng Fan Mingge Han Lanjie Zhao Hong Zhang Xiaoyu Liu Nan Xu Jing Wang Hui Huang Xixian Feng Yanlong Xi Kesong Ni Menghao Zhang Menghao Zhang Tiantian Jiang Wuwei Ye Wuwei Ye |
| author_facet | Yuqian Lei Yuqian Lei Yupeng Cui Ruifeng Cui Xiugui Chen Junjuan Wang Xuke Lu Delong Wang Shuai Wang Lixue Guo Yuexin Zhang Cun Rui Yapeng Fan Mingge Han Lanjie Zhao Hong Zhang Xiaoyu Liu Nan Xu Jing Wang Hui Huang Xixian Feng Yanlong Xi Kesong Ni Menghao Zhang Menghao Zhang Tiantian Jiang Wuwei Ye Wuwei Ye |
| author_sort | Yuqian Lei |
| collection | DOAJ |
| description | Identification, evolution, and expression patterns of BSK (BR signaling kinase) family genes revealed that BSKs participated in the response of cotton to abiotic stress and maintained the growth of cotton in extreme environment. The steroidal hormone brassinosteroids (BR) play important roles in different plant biological processes. This study focused on BSK which were downstream regulatory element of BR, in order to help to decipher the functions of BSKs genes from cotton on growth development and responses to abiotic stresses and lean the evolutionary relationship of cotton BSKs. BSKs are a class of plant-specific receptor-like cytoplasmic kinases involved in BR signal transduction. In this study, bioinformatics methods were used to identify the cotton BSKs gene family at the cotton genome level, and the gene structure, promoter elements, protein structure and properties, gene expression patterns and candidate interacting proteins were analyzed. In the present study, a total of 152 BSKs were identified by a genome-wide search in four cotton species and other 11 plant species, and phylogenetic analysis revealed three evolutionary clades. It was identified that BSKs contain typical PKc and TPR domains, the N-terminus is composed of extended chains and helical structures. Cotton BSKs genes show different expression patterns in different tissues and organs. The gene promoter contains numerous cis-acting elements induced by hormones and abiotic stress, the hormone ABA and Cold-inducing related elements have the highest count, indicating that cotton BSK genes may be regulated by various hormones at different growth stages and involved in the response regulation of cotton to various stresses. The expression analysis of BSKs in cotton showed that the expression levels of GhBSK06, GhBSK10, GhBSK21 and GhBSK24 were significantly increased with salt-inducing. This study is helpful to analyze the function of cotton BSKs genes in growth and development and in response to stress. |
| first_indexed | 2024-03-13T06:58:03Z |
| format | Article |
| id | doaj.art-86c98c285ce040ff86b337701b961f57 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-03-13T06:58:03Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-86c98c285ce040ff86b337701b961f572023-06-07T04:53:05ZengFrontiers Media S.A.Frontiers in Genetics1664-80212023-06-011410.3389/fgene.2023.11691041169104Characterization and gene expression patterns analysis implies BSK family genes respond to salinity stress in cottonYuqian Lei0Yuqian Lei1Yupeng Cui2Ruifeng Cui3Xiugui Chen4Junjuan Wang5Xuke Lu6Delong Wang7Shuai Wang8Lixue Guo9Yuexin Zhang10Cun Rui11Yapeng Fan12Mingge Han13Lanjie Zhao14Hong Zhang15Xiaoyu Liu16Nan Xu17Jing Wang18Hui Huang19Xixian Feng20Yanlong Xi21Kesong Ni22Menghao Zhang23Menghao Zhang24Tiantian Jiang25Wuwei Ye26Wuwei Ye27Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, Henan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaAnyang Institute of Technology, Anyang, Henan, ChinaAnyang Institute of Technology, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, Henan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, Henan, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaIdentification, evolution, and expression patterns of BSK (BR signaling kinase) family genes revealed that BSKs participated in the response of cotton to abiotic stress and maintained the growth of cotton in extreme environment. The steroidal hormone brassinosteroids (BR) play important roles in different plant biological processes. This study focused on BSK which were downstream regulatory element of BR, in order to help to decipher the functions of BSKs genes from cotton on growth development and responses to abiotic stresses and lean the evolutionary relationship of cotton BSKs. BSKs are a class of plant-specific receptor-like cytoplasmic kinases involved in BR signal transduction. In this study, bioinformatics methods were used to identify the cotton BSKs gene family at the cotton genome level, and the gene structure, promoter elements, protein structure and properties, gene expression patterns and candidate interacting proteins were analyzed. In the present study, a total of 152 BSKs were identified by a genome-wide search in four cotton species and other 11 plant species, and phylogenetic analysis revealed three evolutionary clades. It was identified that BSKs contain typical PKc and TPR domains, the N-terminus is composed of extended chains and helical structures. Cotton BSKs genes show different expression patterns in different tissues and organs. The gene promoter contains numerous cis-acting elements induced by hormones and abiotic stress, the hormone ABA and Cold-inducing related elements have the highest count, indicating that cotton BSK genes may be regulated by various hormones at different growth stages and involved in the response regulation of cotton to various stresses. The expression analysis of BSKs in cotton showed that the expression levels of GhBSK06, GhBSK10, GhBSK21 and GhBSK24 were significantly increased with salt-inducing. This study is helpful to analyze the function of cotton BSKs genes in growth and development and in response to stress.https://www.frontiersin.org/articles/10.3389/fgene.2023.1169104/fullBRBSKbrassinosteroid-signaling kinaseabiotic stresssalt stress |
| spellingShingle | Yuqian Lei Yuqian Lei Yupeng Cui Ruifeng Cui Xiugui Chen Junjuan Wang Xuke Lu Delong Wang Shuai Wang Lixue Guo Yuexin Zhang Cun Rui Yapeng Fan Mingge Han Lanjie Zhao Hong Zhang Xiaoyu Liu Nan Xu Jing Wang Hui Huang Xixian Feng Yanlong Xi Kesong Ni Menghao Zhang Menghao Zhang Tiantian Jiang Wuwei Ye Wuwei Ye Characterization and gene expression patterns analysis implies BSK family genes respond to salinity stress in cotton Frontiers in Genetics BR BSK brassinosteroid-signaling kinase abiotic stress salt stress |
| title | Characterization and gene expression patterns analysis implies BSK family genes respond to salinity stress in cotton |
| title_full | Characterization and gene expression patterns analysis implies BSK family genes respond to salinity stress in cotton |
| title_fullStr | Characterization and gene expression patterns analysis implies BSK family genes respond to salinity stress in cotton |
| title_full_unstemmed | Characterization and gene expression patterns analysis implies BSK family genes respond to salinity stress in cotton |
| title_short | Characterization and gene expression patterns analysis implies BSK family genes respond to salinity stress in cotton |
| title_sort | characterization and gene expression patterns analysis implies bsk family genes respond to salinity stress in cotton |
| topic | BR BSK brassinosteroid-signaling kinase abiotic stress salt stress |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2023.1169104/full |
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