Study on Tumor Necrosis Factor-α· Gene Polymorphism in Rheumatoid Arthritis
Objectives : Tumor necrosis factor-(TNF-) is a proinflammatory cytokine involved in the pathogenesis of rheumatoid arthritis. This study was designed to investigate the relation between TNF- gene polymorphism and rheumatoid arthritis in Korean population. Methods : This study was carried out on 103...
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| Format: | Article |
| Language: | English |
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Korean Pharmacopuncture Institute
2007-06-01
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| Series: | Journal of Pharmacopuncture |
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| Online Access: | http://dx.doi.org/10.3831/KPI.2007.10.2.073 |
| _version_ | 1818187571587448832 |
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| author | Kim Kyung-Un Lee Kyung-Min Lee Bong-Hyo Lim Seong-Chul Jung Tae-Young Seo Jung-Chul |
| author_facet | Kim Kyung-Un Lee Kyung-Min Lee Bong-Hyo Lim Seong-Chul Jung Tae-Young Seo Jung-Chul |
| author_sort | Kim Kyung-Un |
| collection | DOAJ |
| description | Objectives : Tumor necrosis factor-(TNF-) is a proinflammatory cytokine involved in the pathogenesis of rheumatoid arthritis. This study was designed to investigate the relation between TNF- gene polymorphism and rheumatoid arthritis in Korean population. Methods : This study was carried out on 103 rheumatoid arthritis patients who fulfilled the American College of Rheumatology 1987 revised criteria for rheumatoid arthritis and 208 healthy control subjects. Blood samples from all subjects were obtained for DNA extraction. The extracted DNA was amplified by polymerse chain reaction(PCR). PCR products were visualized by 2% agarose gel electrophoresis. We investigated the genotyping of TNF- by using Pyrosequencing.
Results : The genotypes of TNF- gene were GG, AG and AA. While the distribution of TNF- polymorphism in control subjects was 92.31%, 7.21%, 0.48% respectively, in rheumatoid arthritis patients was 93.20%, 6.80%, 0.00%(GG, AG. AA). There was no statistical significant allelic frequency difference between control and rheumatoid arthritis groups.
Conclusion : We concluded that there was no significant association between TNF- gene polymorphism and rheumatoid arthritis. However, the findings of this study need to be confirmed in more patients and further studies. |
| first_indexed | 2024-12-11T23:13:09Z |
| format | Article |
| id | doaj.art-86cb971d638f4d8b887257cb8c67f339 |
| institution | Directory Open Access Journal |
| issn | 2093-6966 2234-6856 |
| language | English |
| last_indexed | 2024-12-11T23:13:09Z |
| publishDate | 2007-06-01 |
| publisher | Korean Pharmacopuncture Institute |
| record_format | Article |
| series | Journal of Pharmacopuncture |
| spelling | doaj.art-86cb971d638f4d8b887257cb8c67f3392022-12-22T00:46:39ZengKorean Pharmacopuncture InstituteJournal of Pharmacopuncture2093-69662234-68562007-06-01102737910.3831/KPI.2007.10.2.073 Study on Tumor Necrosis Factor-α· Gene Polymorphism in Rheumatoid ArthritisKim Kyung-Un 0Lee Kyung-Min1Lee Bong-Hyo2Lim Seong-Chul 3Jung Tae-Young 4Seo Jung-Chul 5Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany UniversityDepartment of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany UniversityDepartment of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany UniversityDepartment of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany UniversityDepartment of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany UniversityDepartment of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany UniversityObjectives : Tumor necrosis factor-(TNF-) is a proinflammatory cytokine involved in the pathogenesis of rheumatoid arthritis. This study was designed to investigate the relation between TNF- gene polymorphism and rheumatoid arthritis in Korean population. Methods : This study was carried out on 103 rheumatoid arthritis patients who fulfilled the American College of Rheumatology 1987 revised criteria for rheumatoid arthritis and 208 healthy control subjects. Blood samples from all subjects were obtained for DNA extraction. The extracted DNA was amplified by polymerse chain reaction(PCR). PCR products were visualized by 2% agarose gel electrophoresis. We investigated the genotyping of TNF- by using Pyrosequencing. Results : The genotypes of TNF- gene were GG, AG and AA. While the distribution of TNF- polymorphism in control subjects was 92.31%, 7.21%, 0.48% respectively, in rheumatoid arthritis patients was 93.20%, 6.80%, 0.00%(GG, AG. AA). There was no statistical significant allelic frequency difference between control and rheumatoid arthritis groups. Conclusion : We concluded that there was no significant association between TNF- gene polymorphism and rheumatoid arthritis. However, the findings of this study need to be confirmed in more patients and further studies.http://dx.doi.org/10.3831/KPI.2007.10.2.073Rheumatoid ArthritisTNF-αGenePolymorphism |
| spellingShingle | Kim Kyung-Un Lee Kyung-Min Lee Bong-Hyo Lim Seong-Chul Jung Tae-Young Seo Jung-Chul Study on Tumor Necrosis Factor-α· Gene Polymorphism in Rheumatoid Arthritis Journal of Pharmacopuncture Rheumatoid Arthritis TNF-α Gene Polymorphism |
| title | Study on Tumor Necrosis Factor-α· Gene Polymorphism in Rheumatoid Arthritis |
| title_full | Study on Tumor Necrosis Factor-α· Gene Polymorphism in Rheumatoid Arthritis |
| title_fullStr | Study on Tumor Necrosis Factor-α· Gene Polymorphism in Rheumatoid Arthritis |
| title_full_unstemmed | Study on Tumor Necrosis Factor-α· Gene Polymorphism in Rheumatoid Arthritis |
| title_short | Study on Tumor Necrosis Factor-α· Gene Polymorphism in Rheumatoid Arthritis |
| title_sort | study on tumor necrosis factor α· gene polymorphism in rheumatoid arthritis |
| topic | Rheumatoid Arthritis TNF-α Gene Polymorphism |
| url | http://dx.doi.org/10.3831/KPI.2007.10.2.073 |
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