Immunization of sheep against Echinococcus granulosus with protoscolex tegumental surface antigens
Aim: Cystic echinococcosis (CE) has potential economic effects to both animal products and human health. A vaccine to protect livestock against CE can be effective in reducing economic costs and increasing the livestock products. Protoscolex tegumental surface antigens (PSTSA) used to induce the pro...
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| Format: | Article |
| Language: | English |
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Veterinary World
2017-08-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.10/August-2017/3.pdf |
| _version_ | 1818649259171381248 |
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| author | Manouchehr Valizadeh Behzad Haghpanah Alireza Badirzadeh Elham Roointan Shirzad Fallahi Saber Raeghi |
| author_facet | Manouchehr Valizadeh Behzad Haghpanah Alireza Badirzadeh Elham Roointan Shirzad Fallahi Saber Raeghi |
| author_sort | Manouchehr Valizadeh |
| collection | DOAJ |
| description | Aim: Cystic echinococcosis (CE) has potential economic effects to both animal products and human health. A vaccine to protect livestock against CE can be effective in reducing economic costs and increasing the livestock products. Protoscolex tegumental surface antigens (PSTSA) used to induce the production of specific antibodies against Echinococcus granulosus in sheep. The tegumental antigens were extracted from viable protoscolices by solubilization in sterile phosphate-buffered saline containing decanoyl-N-methylglucamine.
Materials and Methods: Ten lambs which were infected with CE (positive control), 10 negative control, and 10 test groups of sheep were included in the study. 300 μg emulsion of purified-PSTSA was injected intramuscularly in a two-step immunization on the first and 30 days. Sera were collected immediately before immunization and 6 times with 10-day intervals until 60 days post immunization. Thereafter, the sera were tested for antibodies by indirect hemagglutination test in microtiter plate.
Results: After two immunizations, all the infected animals in test group showed substantial increases in antibody titer. Statistical analysis showed a significant difference between the titer obtained in the test and negative control groups in both phases of immunization (p<0.05).
Conclusion: The results showed that the PSTSA is a promising immunogenic compound for immunization of sheep against CE. |
| first_indexed | 2024-12-17T01:31:29Z |
| format | Article |
| id | doaj.art-86d019fb48c1489a81cdf0712613ae55 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-17T01:31:29Z |
| publishDate | 2017-08-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-86d019fb48c1489a81cdf0712613ae552022-12-21T22:08:33ZengVeterinary WorldVeterinary World0972-89882231-09162017-08-0110885485810.14202/vetworld.2017.854-858Immunization of sheep against Echinococcus granulosus with protoscolex tegumental surface antigensManouchehr Valizadeh0Behzad Haghpanah1Alireza Badirzadeh2Elham Roointan3Shirzad Fallahi4Saber Raeghi5Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Department of Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.Department of Laboratory Sciences, Maragheh University of Medical Sciences, Maragheh, Iran.Aim: Cystic echinococcosis (CE) has potential economic effects to both animal products and human health. A vaccine to protect livestock against CE can be effective in reducing economic costs and increasing the livestock products. Protoscolex tegumental surface antigens (PSTSA) used to induce the production of specific antibodies against Echinococcus granulosus in sheep. The tegumental antigens were extracted from viable protoscolices by solubilization in sterile phosphate-buffered saline containing decanoyl-N-methylglucamine. Materials and Methods: Ten lambs which were infected with CE (positive control), 10 negative control, and 10 test groups of sheep were included in the study. 300 μg emulsion of purified-PSTSA was injected intramuscularly in a two-step immunization on the first and 30 days. Sera were collected immediately before immunization and 6 times with 10-day intervals until 60 days post immunization. Thereafter, the sera were tested for antibodies by indirect hemagglutination test in microtiter plate. Results: After two immunizations, all the infected animals in test group showed substantial increases in antibody titer. Statistical analysis showed a significant difference between the titer obtained in the test and negative control groups in both phases of immunization (p<0.05). Conclusion: The results showed that the PSTSA is a promising immunogenic compound for immunization of sheep against CE.http://www.veterinaryworld.org/Vol.10/August-2017/3.pdfcystic echinococcosisEchinococcus granulosusimmunizationIranProtoscolex tegumental surface antigenssheep |
| spellingShingle | Manouchehr Valizadeh Behzad Haghpanah Alireza Badirzadeh Elham Roointan Shirzad Fallahi Saber Raeghi Immunization of sheep against Echinococcus granulosus with protoscolex tegumental surface antigens Veterinary World cystic echinococcosis Echinococcus granulosus immunization Iran Protoscolex tegumental surface antigens sheep |
| title | Immunization of sheep against Echinococcus granulosus with protoscolex tegumental surface antigens |
| title_full | Immunization of sheep against Echinococcus granulosus with protoscolex tegumental surface antigens |
| title_fullStr | Immunization of sheep against Echinococcus granulosus with protoscolex tegumental surface antigens |
| title_full_unstemmed | Immunization of sheep against Echinococcus granulosus with protoscolex tegumental surface antigens |
| title_short | Immunization of sheep against Echinococcus granulosus with protoscolex tegumental surface antigens |
| title_sort | immunization of sheep against echinococcus granulosus with protoscolex tegumental surface antigens |
| topic | cystic echinococcosis Echinococcus granulosus immunization Iran Protoscolex tegumental surface antigens sheep |
| url | http://www.veterinaryworld.org/Vol.10/August-2017/3.pdf |
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