Impacts of the Biocontrol Strain Pseudomonas simiae PICF7 on the Banana Holobiont: Alteration of Root Microbial Co-occurrence Networks and Effect on Host Defense Responses
The impact of the versatile biocontrol and plant-growth-promoting rhizobacteria Pseudomonas simiae PICF7 on the banana holobiont under controlled conditions was investigated. We examine the fate of this biological control agent (BCA) upon introduction in the soil, the effect on the banana root micro...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.809126/full |
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| author | Carmen Gómez-Lama Cabanás Nuria M. Wentzien Yasmín Zorrilla-Fontanesi Antonio Valverde-Corredor Antonio J. Fernández-González Manuel Fernández-López Jesús Mercado-Blanco |
| author_facet | Carmen Gómez-Lama Cabanás Nuria M. Wentzien Yasmín Zorrilla-Fontanesi Antonio Valverde-Corredor Antonio J. Fernández-González Manuel Fernández-López Jesús Mercado-Blanco |
| author_sort | Carmen Gómez-Lama Cabanás |
| collection | DOAJ |
| description | The impact of the versatile biocontrol and plant-growth-promoting rhizobacteria Pseudomonas simiae PICF7 on the banana holobiont under controlled conditions was investigated. We examine the fate of this biological control agent (BCA) upon introduction in the soil, the effect on the banana root microbiota, and the influence on specific host genetic defense responses. While the presence of strain PICF7 significantly altered neither the composition nor the structure of the root microbiota, a significant shift in microbial community interactions through co-occurrence network analysis was observed. Despite the fact that PICF7 did not constitute a keystone, the topology of this network was significantly modified—the BCA being identified as a constituent of one of the main network modules in bacterized plants. Gene expression analysis showed the early suppression of several systemic acquired resistance and induced systemic resistance (ISR) markers. This outcome occurred at the time in which the highest relative abundance of PICF7 was detected. The absence of major and permanent changes on the banana holobiont upon PICF7 introduction poses advantages regarding the use of this beneficial rhizobacteria under field conditions. Indeed a BCA able to control the target pathogen while altering as little as possible the natural host-associated microbiome should be a requisite when developing effective bio-inoculants. |
| first_indexed | 2024-12-13T01:01:32Z |
| format | Article |
| id | doaj.art-86d06d05009a4d939268c6bbd46c2397 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T01:01:32Z |
| publishDate | 2022-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-86d06d05009a4d939268c6bbd46c23972022-12-22T00:04:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-02-011310.3389/fmicb.2022.809126809126Impacts of the Biocontrol Strain Pseudomonas simiae PICF7 on the Banana Holobiont: Alteration of Root Microbial Co-occurrence Networks and Effect on Host Defense ResponsesCarmen Gómez-Lama Cabanás0Nuria M. Wentzien1Yasmín Zorrilla-Fontanesi2Antonio Valverde-Corredor3Antonio J. Fernández-González4Manuel Fernández-López5Jesús Mercado-Blanco6Departamento de Protección de Cultivos, Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Córdoba, SpainDepartamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, SpainLaboratory of Tropical Crop Improvement, Division of Crop Biotechnics, KU Leuven, Leuven, BelgiumDepartamento de Protección de Cultivos, Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Córdoba, SpainDepartamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, SpainDepartamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, SpainDepartamento de Protección de Cultivos, Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Córdoba, SpainThe impact of the versatile biocontrol and plant-growth-promoting rhizobacteria Pseudomonas simiae PICF7 on the banana holobiont under controlled conditions was investigated. We examine the fate of this biological control agent (BCA) upon introduction in the soil, the effect on the banana root microbiota, and the influence on specific host genetic defense responses. While the presence of strain PICF7 significantly altered neither the composition nor the structure of the root microbiota, a significant shift in microbial community interactions through co-occurrence network analysis was observed. Despite the fact that PICF7 did not constitute a keystone, the topology of this network was significantly modified—the BCA being identified as a constituent of one of the main network modules in bacterized plants. Gene expression analysis showed the early suppression of several systemic acquired resistance and induced systemic resistance (ISR) markers. This outcome occurred at the time in which the highest relative abundance of PICF7 was detected. The absence of major and permanent changes on the banana holobiont upon PICF7 introduction poses advantages regarding the use of this beneficial rhizobacteria under field conditions. Indeed a BCA able to control the target pathogen while altering as little as possible the natural host-associated microbiome should be a requisite when developing effective bio-inoculants.https://www.frontiersin.org/articles/10.3389/fmicb.2022.809126/fullbeneficial rhizobacteriabiocontrol agentsco-occurrence networksdefense-related genesmicrobiomeMusa acuminata |
| spellingShingle | Carmen Gómez-Lama Cabanás Nuria M. Wentzien Yasmín Zorrilla-Fontanesi Antonio Valverde-Corredor Antonio J. Fernández-González Manuel Fernández-López Jesús Mercado-Blanco Impacts of the Biocontrol Strain Pseudomonas simiae PICF7 on the Banana Holobiont: Alteration of Root Microbial Co-occurrence Networks and Effect on Host Defense Responses Frontiers in Microbiology beneficial rhizobacteria biocontrol agents co-occurrence networks defense-related genes microbiome Musa acuminata |
| title | Impacts of the Biocontrol Strain Pseudomonas simiae PICF7 on the Banana Holobiont: Alteration of Root Microbial Co-occurrence Networks and Effect on Host Defense Responses |
| title_full | Impacts of the Biocontrol Strain Pseudomonas simiae PICF7 on the Banana Holobiont: Alteration of Root Microbial Co-occurrence Networks and Effect on Host Defense Responses |
| title_fullStr | Impacts of the Biocontrol Strain Pseudomonas simiae PICF7 on the Banana Holobiont: Alteration of Root Microbial Co-occurrence Networks and Effect on Host Defense Responses |
| title_full_unstemmed | Impacts of the Biocontrol Strain Pseudomonas simiae PICF7 on the Banana Holobiont: Alteration of Root Microbial Co-occurrence Networks and Effect on Host Defense Responses |
| title_short | Impacts of the Biocontrol Strain Pseudomonas simiae PICF7 on the Banana Holobiont: Alteration of Root Microbial Co-occurrence Networks and Effect on Host Defense Responses |
| title_sort | impacts of the biocontrol strain pseudomonas simiae picf7 on the banana holobiont alteration of root microbial co occurrence networks and effect on host defense responses |
| topic | beneficial rhizobacteria biocontrol agents co-occurrence networks defense-related genes microbiome Musa acuminata |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.809126/full |
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