Effect of tamarillo (Cyphomandra betacea Sendtn.) seed ethanol extract on HSC-3 tongue cancer cells
Background: Cancer is characterized by abnormal cell growth. Squamous cell carcinoma is the most common type oral cancer. Tamarillo fruit extract shows antiproliferative and antioxidant activity against breast cancer (MDA-MB-231) and liver cancer (HepG2) cells in rat models. Tamarillo seed extract c...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Scientific Dental Journal |
| Subjects: | |
| Online Access: | http://www.scidentj.com/article.asp?issn=2580-6548;year=2022;volume=6;issue=3;spage=141;epage=145;aulast=Salfabila |
| _version_ | 1811200247080157184 |
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| author | Saqila Salfabila Janti Sudiono |
| author_facet | Saqila Salfabila Janti Sudiono |
| author_sort | Saqila Salfabila |
| collection | DOAJ |
| description | Background: Cancer is characterized by abnormal cell growth. Squamous cell carcinoma is the most common type oral cancer. Tamarillo fruit extract shows antiproliferative and antioxidant activity against breast cancer (MDA-MB-231) and liver cancer (HepG2) cells in rat models. Tamarillo seed extract consists of phenolics, flavonoids, triterpenoids, tannins, and alkaloids, which have antioxidant, antiproliferative, anti-inflammatory, and anticancer activity. Objectives: The aim of this study was to determine the effect of tamarillo seed ethanol extract at different concentrations on the viability of a tongue cancer cell (human oral squamous carcinoma cell line HSC-3). Methods: This experimental in vitro laboratory study comprised a treatment group comprising HSC-3 cells treated with eight concentrations of tamarillo seed extract (8, 4, 2, 1, 0.5, 0.25, 0.125, and 0.0625 μg/µL) and a positive control group treated with 3% H2O2. Shapiro–Wilk test showed that the data were distributed normally with P > 0.05. One-way analysis of variance (ANOVA) test showed that there were significant differences among concentrations group of the extract on HSC-3 viability cells with P ≤ 0.05. Results: At a concentration of 2 μg/µL, tamarillo seed ethanol extract showed the highest cytotoxicity against HSC-3 cells. Post hoc test revealed a significant difference (P < 0.05) in cell viability of concentration 2 μg/µL compared with that of the lower concentrations (0.25, 0.125, and 0.0625 μg/µL) and the positive control but there was no significant difference with those of higher concentration (4 and 8 μg/µL). Conclusions: A low concentration of tamarillo seed ethanol extract (2 µg/µL) had the highest cytotoxicity against HSC-3 cells significantly reducing cell viability. However, this concentration was not able to surpass the inhibition ability of the positive control (3% H2O2). |
| first_indexed | 2024-04-12T02:01:25Z |
| format | Article |
| id | doaj.art-86d1819980a444baafd4c4f13b68f8d4 |
| institution | Directory Open Access Journal |
| issn | 2580-6548 2541-321X |
| language | English |
| last_indexed | 2024-04-12T02:01:25Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Scientific Dental Journal |
| spelling | doaj.art-86d1819980a444baafd4c4f13b68f8d42022-12-22T03:52:40ZengWolters Kluwer Medknow PublicationsScientific Dental Journal2580-65482541-321X2022-01-016314114510.4103/SDJ.SDJ_24_22Effect of tamarillo (Cyphomandra betacea Sendtn.) seed ethanol extract on HSC-3 tongue cancer cellsSaqila SalfabilaJanti SudionoBackground: Cancer is characterized by abnormal cell growth. Squamous cell carcinoma is the most common type oral cancer. Tamarillo fruit extract shows antiproliferative and antioxidant activity against breast cancer (MDA-MB-231) and liver cancer (HepG2) cells in rat models. Tamarillo seed extract consists of phenolics, flavonoids, triterpenoids, tannins, and alkaloids, which have antioxidant, antiproliferative, anti-inflammatory, and anticancer activity. Objectives: The aim of this study was to determine the effect of tamarillo seed ethanol extract at different concentrations on the viability of a tongue cancer cell (human oral squamous carcinoma cell line HSC-3). Methods: This experimental in vitro laboratory study comprised a treatment group comprising HSC-3 cells treated with eight concentrations of tamarillo seed extract (8, 4, 2, 1, 0.5, 0.25, 0.125, and 0.0625 μg/µL) and a positive control group treated with 3% H2O2. Shapiro–Wilk test showed that the data were distributed normally with P > 0.05. One-way analysis of variance (ANOVA) test showed that there were significant differences among concentrations group of the extract on HSC-3 viability cells with P ≤ 0.05. Results: At a concentration of 2 μg/µL, tamarillo seed ethanol extract showed the highest cytotoxicity against HSC-3 cells. Post hoc test revealed a significant difference (P < 0.05) in cell viability of concentration 2 μg/µL compared with that of the lower concentrations (0.25, 0.125, and 0.0625 μg/µL) and the positive control but there was no significant difference with those of higher concentration (4 and 8 μg/µL). Conclusions: A low concentration of tamarillo seed ethanol extract (2 µg/µL) had the highest cytotoxicity against HSC-3 cells significantly reducing cell viability. However, this concentration was not able to surpass the inhibition ability of the positive control (3% H2O2).http://www.scidentj.com/article.asp?issn=2580-6548;year=2022;volume=6;issue=3;spage=141;epage=145;aulast=Salfabilahsc-3 human tongue squamous carcinoma cell lineseed extracttamarilloviability |
| spellingShingle | Saqila Salfabila Janti Sudiono Effect of tamarillo (Cyphomandra betacea Sendtn.) seed ethanol extract on HSC-3 tongue cancer cells Scientific Dental Journal hsc-3 human tongue squamous carcinoma cell line seed extract tamarillo viability |
| title | Effect of tamarillo (Cyphomandra betacea Sendtn.) seed ethanol extract on HSC-3 tongue cancer cells |
| title_full | Effect of tamarillo (Cyphomandra betacea Sendtn.) seed ethanol extract on HSC-3 tongue cancer cells |
| title_fullStr | Effect of tamarillo (Cyphomandra betacea Sendtn.) seed ethanol extract on HSC-3 tongue cancer cells |
| title_full_unstemmed | Effect of tamarillo (Cyphomandra betacea Sendtn.) seed ethanol extract on HSC-3 tongue cancer cells |
| title_short | Effect of tamarillo (Cyphomandra betacea Sendtn.) seed ethanol extract on HSC-3 tongue cancer cells |
| title_sort | effect of tamarillo cyphomandra betacea sendtn seed ethanol extract on hsc 3 tongue cancer cells |
| topic | hsc-3 human tongue squamous carcinoma cell line seed extract tamarillo viability |
| url | http://www.scidentj.com/article.asp?issn=2580-6548;year=2022;volume=6;issue=3;spage=141;epage=145;aulast=Salfabila |
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